Data from: Long-term soil warming decreases microbial phosphorus utilization by increasing abiotic phosphorus sorption and losses

Tian, Ye 1 ; Wanek, Wolfgang 1

Published Feb 03, 2023; Updated Apr 30, 2026 on Dryad. https://doi.org/10.5061/dryad.9p8cz8wk8

Data files

Feb 03, 2023 version files 54.83 KB

README.md

3 KB
Source_Data.xlsx

51.82 KB

Apr 30, 2026 version files 55.99 KB

README.md

3.89 KB
Source_Data_updated.xlsx

52.10 KB

Abstract

Phosphorus (P) is an essential and often limiting element that could play a crucial role in terrestrial ecosystem responses to climate warming. However, it has yet remained unclear how different P cycling processes are affected by warming. Here we investigated the response of soil P pools and P cycling processes in a mountain forest after 14 years of soil warming (+4°C). Long-term warming decreased soil total P pools, likely due to higher outputs of P from soils by increasing net plant P uptake and downward transportation of colloidal and particulate P. Warming increased the sorption strength to more recalcitrant soil P fractions (absorbed to iron oxyhydroxides and clays), thereby further reducing bioavailable P in soil solution. As a response, soil microbes enhanced the production of acid phosphatase, though this was not sufficient to avoid decreases of soil bioavailable P and microbial biomass P (and biotic phosphate immobilization). This study therefore highlights how long-term soil warming triggers changes in biotic and abiotic soil P pools and processes, which can potentially aggravate the P constraints of the trees and soil microbes and thereby negatively affect the C sequestration potential of these forests.

Dataset (see Source_Data_updated.xlsx) for the manuscript, Long-term soil warming decreases microbial phosphorus utilization by increasing abiotic phosphorus sorption and losses, authored by Tian et al. (2023).

Sheet 1

In the sheet, Mastersheet, columns H to Z are the parameters that were measured with the seasonal samples in 2019 (n = 72), and columns AA to AR are the parameters that were sampled with the samples from August 2020 (n = 24, and thus AA26 to AR73 are n/a).

The unit of parameter:

Duration: year
Depth: cm
pH: n/a
SWC (soil water content): g H2O/g d.s. (dry soil weight)
AP (potential activity of acid phosphatase): nmol/h/g d.s.
SOC (soil organic carbon): g C/kg d.s.
TN (soil total nitrogen): g N/kg d.s.
TP (soil total phosphorus): g P/kg d.s.
TIP (soil total inorganic phosphorus): mg P/kg d.s.
TOP (soil total organic phosphorus): mg P/kg d.s.
OlsenP (Olsen total phosphorus): g P/g d.s.
OlsenPi (Olsen inorganic phosphorus): g P/g d.s.
Olsen Po (Olsen organic phosphorus): g P/g d.s.
MBC (microbial biomass carbon): g C/g d.s.
MBN (microbial biomass nitrogen): g N/g d.s.
MBP (microbial biomass phosphorus): g P/g d.s.
abiotic_immobilization (net abiotic phosphate immobilization): %
biotic_immobilization (net biotic phosphate immobilization): %
gross_P_mobilization (gross phosphate mobilization rate): g P/g d.s./d
gross_P_immobilization (gross phosphate immobilization rate): g P/g d.s./d
Fe_oxalate (oxalate extractable Fe): mg FeO/g d.s.
Mn_oxalate (oxalate extractable Mn): mg MnO/g d.s.
Al_oxalate (oxalate extractable Al): mg AlO/g d.s.
Fe_dithionite (dithionite extractable Fe): mg Fe/g d.s.
Mn_dithionite (dithionite extractable Mn): mg Mn/g d.s.
Fe_crystalline (crystalline Fe): mg Fe/g d.s.
sand: %
silt: %
clay: %
eNa (exchangeable Na): mmol cation/kg d.s.
eK (exchangeable K): mmol cation/kg d.s.
eCa (exchangeable Ca): mmol cation/kg d.s.
eMg (exchangeable Mg): mmol cation/kg d.s.
eMn (exchangeable Mn): mmol cation/kg d.s.
CEC (cation exchange capacity): mmol cation/kg d.s.
kaolinite: %
illite: %
Chlorite_mixed_layer (Chlorite and mixed layer): %

Sheet 2 to 4

Sheets, Fig2_PCA_data, Fig2_PCA_group, and Fig2_PCA_property, were utilized to perform the principal component analysis in R. The units of the parameters in Fig2_PCA_data are the same as the units of corresponding parameters in Sheet 1.

Note on red-font cells (Fig2_PCA_data): in the sheet “Fig2_PCA_data”, red-font cells indicate values that were originally missing due to CO₂ generation during H₂SO₄ extraction in calcareous soil samples. In the dataset, the missing value was replaced with the mean of the corresponding samples, and are highlighted in red for transparency.

Sheet 5

In the sheet, Fig3, we used the averaged seasonal data from 2019 in columns A, B, and D (n =24) against the one season data from 2020 in column C (n=24) to generate Fig. 3a, 3b, and 3c. The data in columns E and F (n =72) were used to generate the Fig. 3d. Data from A26 to D73 are n/a due to using the averaged values or having smaller sample size than the data in columns E and F. The abbreviations and units in Sheet 1 also apply in this sheet, except for that TDP in column A and MBP in column E were log-transformed, and MBP in column D was sqrt-tranformed.

Changes made in this version:

Changes were made in the following sheets: Mastersheet, Fig2_PCA_data, and Fig3. These changes include replacing previously mean-substituted total soil phosphorus (TP), total soil inorganic phosphorus (TIP), and total soil organic phosphorus (TOP) values with NA, and correcting oxalate-extractable Fe and derived crystalline Fe oxide values.