Phytoplankton optical fingerprint libraries for development of phytoplankton ocean color satellite products
Data files
Dec 22, 2023 version files 39.43 MB
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PLOPS_Data_27Sept2023_Final.xlsx
39.41 MB
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README.md
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Abstract
Quantifying changes in phytoplankton communities using ocean color is essential for predicting ocean food resources, occurrences of harmful algal blooms, and carbon and other elemental cycles, among other predictions. Here we present a dataset of greater than fifty strains of phytoplankton, from a range of taxonomic lineages, geographic locations, and time in culture, alone and in mixtures, grown to exponential and/or stationary phase for determination of hyperspectral UV-VIS absorption coefficients, multi-angle and multi-spectral backscatter coefficients, volume scattering functions, particle size distributions, fluorescence, and hyperspectral remote sensing reflectance. The measurements obtained from these experiments are valuable to facilitate development of new global and/or regional ocean color models by the broader scientific community.
Phytoplankton Optical Fingerprint Libraries for Development of Phytoplankton Ocean Color Satellite Products
Michael W. Lomas1, Aimee R. Neeley2, Ryan Vandermeulen2,*, Antonio Mannino3, Crystal Thomas2, Michael G. Novak2, Scott A. Freeman2
1 Bigelow Institute of Ocean Sciences, Bigelow Laboratory for Ocean 6Sciences, East Boothbay, ME 04544, USA
2 NASA Goddard Space Flight Center/Science Systems and Applications Inc., Greenbelt, Maryland 20771, USA
3 NASA Goddard Space Flight Center, Greenbelt, Maryland 20771, USA
- Current Address: NOAA National Marine Fisheries Service, Silver Spring, Maryland 20910, USA.
Contact Michael W. Lomas (mlomas@bigelow.org)for any questions about the data.
---- Description ----
The dataset comprises bio-optical data collected from 50+ pure phytoplankton cultures that represent broad taxonomic diversity from a broad geographic range of original isolation. Data were collected from phytoplankton cultures in exponential and stationary growth phase, from different known mixtures of cultures, from cultures with different durations in culture, and under climate change growth conditions of elevated temperature, irradiance and CO2.
The dataset includes the following fields:
I. ap_ACS worksheet: particle absorption coefficients from ac-s instrument
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-142. ap-ACS ### (m-1): ac-s particle absorption coefficient every 2.5nm between wavelengths of 400-730nm
inclusive in units of m-1.
II. cp_ACS worksheet: particle beam attenuation coefficients from ac-s
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-142. cp ### (m-1): ac-s beam attenuation coefficient every 2.5nm between wavelengths of 400-730nm inclusive in
units of m-1.
III. Backscatter worksheet: particulate scattering coefficients
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
- bb/b: ratio of backscattering coefficient to scattering coefficient
- bb/b - stdev: standard deviation of ratio of backscattering coefficient to scattering coefficient
- HS6-bb 375 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- HS6-bb 440 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- HS6-bb 488 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- HS6-bb 550 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- HS6-bb 620 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- HS6-bb 700 (m-1): particulate backscatter coefficient at wavelength ### from Hydroscat-6
- VSF3-bb 440 (m-1): particulate backscatter coefficient at wavelength ### from VSF-3
- VSF3-bb 532 (m-1): particulate backscatter coefficient at wavelength ### from VSF-3
- VSF3-bb 660 (m-1): particulate backscatter coefficient at wavelength ### from VSF-3
- VSFR-bb 650 (m-1): particulate backscatter coefficient at wavelength ### from VSF-red
- BB3-bb 470 (m-1): particulate backscatter coefficient at wavelength ### from BB3
- BB3-bb 532 (m-1): particulate backscatter coefficient at wavelength ### from BB3
- BB3-bb 660 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 409 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 441 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 488 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 508 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 526 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 594 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 652 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 679 (m-1): particulate backscatter coefficient at wavelength ### from BB9
- BB9-bb 717 (m-1): particulate backscatter coefficient at wavelength ### from BB9
IV. LISST worksheet: phytoplankton size distribution and bio-volume (LISST)
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
- Volume (mL L-1) 1.36: Particle volume in specified size bin ### um
- Volume (mL L-1) 1.6: Particle volume in specified size bin ### um
- Volume (mL L-1) 1.89: Particle volume in specified size bin ### um
- Volume (mL L-1) 2.23: Particle volume in specified size bin ### um
- Volume (mL L-1) 2.63: Particle volume in specified size bin ### um
- Volume (mL L-1) 3.11: Particle volume in specified size bin ### um
- Volume (mL L-1) 3.67: Particle volume in specified size bin ### um
- Volume (mL L-1) 4.33: Particle volume in specified size bin ### um
- Volume (mL L-1) 5.11: Particle volume in specified size bin ### um
- Volume (mL L-1) 6.03: Particle volume in specified size bin ### um
- Volume (mL L-1) 7.11: Particle volume in specified size bin ### um
- Volume (mL L-1) 8.39: Particle volume in specified size bin ### um
- Volume (mL L-1) 9.9: Particle volume in specified size bin ### um
- Volume (mL L-1) 11.7: Particle volume in specified size bin ### um
- Volume (mL L-1) 13.8: Particle volume in specified size bin ### um
- Volume (mL L-1) 16.3: Particle volume in specified size bin ### um
- Volume (mL L-1) 19.2: Particle volume in specified size bin ### um
- Volume (mL L-1) 22.7: Particle volume in specified size bin ### um
- Volume (mL L-1) 26.7: Particle volume in specified size bin ### um
- Volume (mL L-1) 31.6: Particle volume in specified size bin ### um
- Volume (mL L-1) 37.2: Particle volume in specified size bin ### um
- Volume (mL L-1) 43.9: Particle volume in specified size bin ### um
- Volume (mL L-1) 51.9: Particle volume in specified size bin ### um
- Volume (mL L-1) 61.2: Particle volume in specified size bin ### um
- Volume (mL L-1) 72.2: Particle volume in specified size bin ### um
- Volume (mL L-1) 85.2: Particle volume in specified size bin ### um
- Volume (mL L-1) 101: Particle volume in specified size bin ### um
- Volume (mL L-1) 119: Particle volume in specified size bin ### um
- Volume (mL L-1) 140: Particle volume in specified size bin ### um
- Volume (mL L-1) 165: Particle volume in specified size bin ### um
- Volume (mL L-1) 195: Particle volume in specified size bin ### um
- Volume (mL L-1) 230: Particle volume in specified size bin ### um
- N(D) (particles m-3) 1.36: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 1.6: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 1.89: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 2.23: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 2.63: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 3.11: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 3.67: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 4.33: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 5.11: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 6.03: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 7.11: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 8.39: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 9.9: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 11.7: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 13.8: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 16.3: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 19.2: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 22.7: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 26.7: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 31.6: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 37.2: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 43.9: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 51.9: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 61.2: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 72.2: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 85.2: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 101: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 119: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 140: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 165: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 195: Number of particles per m3 in specified size bin ### um
- N(D) (particles m-3) 230: Number of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 1.36: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 1.6: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 1.89: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 2.23: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 2.63: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 3.11: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 3.67: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 4.33: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 5.11: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 6.03: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 7.11: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 8.39: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 9.9: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 11.7: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 13.8: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 16.3: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 19.2: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 22.7: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 26.7: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 31.6: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 37.2: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 43.9: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 51.9: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 61.2: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 72.2: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 85.2: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 101: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 119: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 140: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 165: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 195: Surface area in m2 of particles per m3 in specified size bin ### um
- A(D) (m2 m-3) 230: Surface area in m2 of particles per m3 in specified size bin ### um
- N(D)_slope: Gamma = slope of the particle size distribution unitless
- Average Diameter um: LISST 100x particle mean diameter
- Beam_C_LISST 665 (m-1): beam attenuation at 665 nm from LISST-100X
V. POC and Chlorophyll-a worksheet (POC_chla):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
- Cells/L: Number of phytoplankton cells per Liter
- POC (ug/L): Particulate Organic Carbon in ug/L
- POC_SD (ug/L): Standard deviation of POC
- POC:Tchla (ug/ug): Ratio of POC to total chlorophyll-a
- POC:Tchla_SD (ug/ug): Standard deviation of ratio
- TChl a (mg/m3): Pigment concentration in mg/m3
- Tchla_SD: Standard deviation of pigment concentration in mg/m^3
VI. HPLC Pigments and ratios worksheet (HPLC):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
- TChl a: Pigment concentration in mg/m^3
- Tchla_SD: Standard deviation of pigment concentration in mg/m^3
- TChl b: Pigment concentration in mg/m^3
- TChlb_SD: Standard deviation of pigment concentration in mg/m^3
- TChl c: Pigment concentration in mg/m^3
- TChlc_SD: Standard deviation of pigment concentration in mg/m^3
- Car: Pigment concentration in mg/m^3
- Car_SD: Standard deviation of pigment concentration in mg/m^3
- But fuco: Pigment concentration in mg/m^3
- Butfuco_SD: Standard deviation of pigment concentration in mg/m^3
- Hex fuco: Pigment concentration in mg/m^3
- Hex fuco_SD: Standard deviation of pigment concentration in mg/m^3
- Allo: Pigment concentration in mg/m^3
- Allo_SD: Standard deviation of pigment concentration in mg/m^3
- Diad: Pigment concentration in mg/m^3
- Diad_SD: Standard deviation of pigment concentration in mg/m^3
- Diato: Pigment concentration in mg/m^3
- Diato_SD: Standard deviation of pigment concentration in mg/m^3
- Fuco: Pigment concentration in mg/m^3
- Fuco_SD: Standard deviation of pigment concentration in mg/m^3
- Perid: Pigment concentration in mg/m^3
- Peri_SD: Standard deviation of pigment concentration in mg/m^3
- Zea: Pigment concentration in mg/m^3
- Zea_SD: Standard deviation of pigment concentration in mg/m^3
- Chl a: Pigment concentration in mg/m^3
- Chl a_SD: Standard deviation of pigment concentration in mg/m^3
- DVChl a: Pigment concentration in mg/m^3
- DVChl a_SD: Standard deviation of pigment concentration in mg/m^3
- Chl b: Pigment concentration in mg/m^3
- Chl b_SD: Standard deviation of pigment concentration in mg/m^3
- DVChl b: Pigment concentration in mg/m^3
- DVChl b_SD: Standard deviation of pigment concentration in mg/m^3
- Chl c1c2: Pigment concentration in mg/m^3
- Chl c1c2_SD: Standard deviation of pigment concentration in mg/m^3
- Chl c3: Pigment concentration in mg/m^3
- Chl c3_SD: Standard deviation of pigment concentration in mg/m^3
- Lut: Pigment concentration in mg/m^3
- Lut_SD: Standard deviation of pigment concentration in mg/m^3
- Neo: Pigment concentration in mg/m^3
- Neo_SD: Standard deviation of pigment concentration in mg/m^3
- Viola: Pigment concentration in mg/m^3
- Viola_SD: Standard deviation of pigment concentration in mg/m^3
- Phytin a: Pigment concentration in mg/m^3
- Phytin a_SD: Standard deviation of pigment concentration in mg/m^3
- Phide a: Pigment concentration in mg/m^3
- Phide a_SD: Standard deviation of pigment concentration in mg/m^3
- Pras: Pigment concentration in mg/m^3
- Pras_SD: Standard deviation of pigment concentration in mg/m^3
- [Gyr diester]: Pigment concentration in mg/m^3
- Gyr diester_SD: Standard deviation of pigment concentration in mg/m^3
- Loroxanthin: Pigment concentration in mg/m^3
- Loro_SD: Standard deviation of pigment concentration in mg/m^3
VII. Filter pad integrating sphere particle absorption worksheet:
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): particle absorption coefficient at wavelength every 0.2nm between 300-800nm inclusive in
units of m-1.
VIII. Filter pad integrating sphere phytoplankton absorption worksheet (aph-spec):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): standard deviation of particle absorption coefficient every 0.2 nm between wavelengths of
300.0-800.0 nm inclusive in units of m-1.
IX. Filter pad integrating sphere de-pigmented particle absorption worksheet (ad-spec):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): de-pigmented particle absorption coefficient every 0.2 nm between wavelengths of 300.0-800.0
nm inclusive in units of m-1.
X. Filter pad integrating sphere de-pigmented particle absorption uncertainty worksheet (ad_spec_unc):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): standard deviation of de-pigmented particle absorption coefficient every 0.2 nm between
wavelengths of 300.0-800.0 nm inclusive in units of m-1.
XI. Filter pad integrating sphere phytoplankton absorption worksheet (aph-spec):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): Phytoplankton absorption coefficient every 0.2 nm between wavelengths of 300.0-800.0 nm
inclusive in units of m-1.
XII. Filter pad integrating sphere phytoplankton absorption uncertainty worksheet (aph_spec_unc):
- EXP: Experiment ID
- CCMP: Strain #### designated by CCMP
- Strain name(s):
- Oceanic/Coastal:
- Class: Phytoplankton strain taxonomic class
- Phase: Growth phase of culture when sampled
- Dilution: Designation of dilution
- Date (MM/DD/YYYY): Date of experiment
- Date (YYYYMMDD): Date of experiment
10-2510. ###.# (m-1): Standard deviation of Phytoplankton absorption coefficient every 0.2 nm between wavelengths
of 300.0-800.0 nm inclusive in units of m-1.
When possible, replicate analyses were conducted for each sample type. Here, in this paper, we report the mean values of the replicate samples.
For all the sample data, "NA" indicate no data or not defined.
A range of experimental conditions were used to assess changes in cellular carbon, pigments, ratios and optical properties. For most of the strains, measurements were made at two growth stages: mid-exponential (Exp) and stationary (Stat) growth phase, but for some strains, measurements were made during only one growth phase. A range of mixed cultures were made to assess the ability of algorithms derived from individual cultures to resolve the known algal components in the prepared mixtures. The potential for changes in pigment composition in response to in vitro culture adaption over time was assessed by studying multiple strains of the same species (Amphidinium carterae, Ditylum brightwellii, and Heterosigma akashiwo) isolated on different historical dates, but from similar geographic areas. Synechococcus sp., Thalassiosira oceanica, and Ostreococcus lucimarinus were examined for their response in pigment composition, cellular carbon, and optical properties to ‘climate change scenarios’ of elevated temperature, irradiance and decreased pH. More detail on these manipulations can be found in (Neeley et al. 2022).
Methods are summarized here with more detail found in Neeley et al. (2022) and Lomas et al. (2023). Samples for pigment concentration were analyzed by HPLC (Agilent RR1200 HPLC) and calculated from pigment response factors, extraction volumes determined from a Vitamin E acetate standard and filtration volumes. Measurements of filter pad particle optical density (ODfp) were performed using a Cary 4000 UV-Visible scanning spectrophotometer equipped with a 15 cm integrating sphere (Labsphere DRA-CA-900), betwee 290-850 nm with a 2nm Slit Band Width (SBW), 0.2 nm data interval, reduced slit height and 120 nm per minute scan speed. Dissolved Organic Carbon measurements were made on Shimadzu TOC-L or TOC- V unit equipped with a total nitrogen analyzer using the high temperature combustion catalytic oxidation method. CDOM absorbance spectra are measured on a double-beam Cary 100 Ultraviolet-Visible scanning spectrophotometer using Suprasil® quartz cylindrical cells of 100 mm pathlength with ultraviolet oxidized Milli-Q water as the blank and reference. Samples for particulate organic nitrogen and carbon concentrations were filtered onto 25 mm precombusted Ahlstrom glass fiber filters (nominal pore size 0.7 µm) under low pressure (~125 mm Hg), and rinsed with 0.2 µm-filtered dilution seawater. Blanks were created by rinsing a pre-combusted filter with filtered dilution seawater to account for any DOC adsorption. A benchtop flow-through apparatus was constructed to measure the inherent optical properties (IOPs) of the diluted phytoplankton cultures. All optical data were processed as described in detail in Lomas et al. 202x.
Usage Notes
This dataset represents a taxonomically and geographically broad dataset of multiple phytoplankton optical parameters. The dataset can be used to validate regional to global scale phytoplankton community composition models, as well as develop new models focused on resolving different phytoplankton within the same taxonomic lineage.
- Lomas, Michael W.; Neeley, Aimee R.; Vandermeulen, Ryan et al. (2024). Phytoplankton optical fingerprint libraries for development of phytoplankton ocean color satellite products. Scientific Data. https://doi.org/10.1038/s41597-024-03001-z
- Neeley, Aimee R.; Lomas, Michael W.; Mannino, Antonio et al. (2022). Impact of Growth Phase, Pigment Adaptation, and Climate Change Conditions on the Cellular Pigment and Carbon Content of Fifty‐One Phytoplankton Isolates. Journal of Phycology. https://doi.org/10.1111/jpy.13279
