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We have submitted the raw data and processed data for Figure1 (Relative expression levels of UGT344J7), Figure2 (RNA interference efficiency), Figure3 (Mortality of R. padi under different treatments), Figure4 (Relative expression levels of Hsp genes), Figure5 (The ROS content), FigureS1 (The relative expression of 43 UGT genes after prolonged high temperature treatment), FigureS2 (Mortality under normal conditions following injection of dsRNA). We mainly used RT-qPCR, RNAi interference, and ELISA to study the role of UGT344J7 in the response of the bird cherry-oat aphid to starvation and high temperature stress. The following is a description of the data set.

 

Processed_data_after_raw_data-Figure1-Relative-expression-levels-of-UGT344J7.csv

Raw_data_for-Figure1-Relative-expression-levels-of-UGT344J7.csv

Data brief description: In Figure 1, we treated aphids with temperature and starvation, and then measured the relative expression of UGT344J7 gene after different treatments. The detection method used was RT-qPCR. Each treatment had three mechanical and three biological replicates. The ct value is the original data detected by the instrument. After calculation by the formula, 2^-∆∆Ct is obtained. We calculated the relative expression of the target gene according to the 2^-∆∆Ct. We selected EF-1a and β-actin as internal reference genes.

Ct: The number of threshold cycles, the number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold.

2^-∆∆Ct: The gene expression level change of the treatment group relative to the control group.

The naming of different treatments is as follows:

C represents the control group (It is worth noting that we made corresponding controls at different temperatures and different starvation treatments. In the file to C1, C2, C3, C4 to distinguish. C1/2: When the treatment conditions were 36℃/38℃ for 2h/4h, the corresponding control group. C3: When the treatment conditions were Starvation treatment for 24h and 24-h starvation and 24-h refeeding, the corresponding control group. C4: When the treatment conditions were Starvation treatment for 48h and 48-h starvation and 24-h refeeding, the corresponding control group.)

36 and 38 represent the treatment temperature of 36℃ and 38℃, respectively; 2h and 4h represent temperature treatment time; S24 and S48 represent hunger for 24 hours and 48 hours, respectively; S24F24: 24-h starvation and 24-h refeeding. S48F24: 48-h starvation and 24-h refeeding.

For example, C1-2h-EF1a-1 represents the first biological duplication of EF1a gene after 2 hours of treatment at control temperature.

 

Processed_data_after_raw_data-Figure2-RNA-interference-efficiency.csv

Raw_data_for-Figure2-RNA-interference-efficiency.csv

Data brief description: In Figure 2, we injected dsRNA into aphids to detect its interference efficiency on UGT344J7 gene. The detection method used was RT-qPCR. Each treatment had three mechanical and three biological replicates. The ct value is the original data detected by the instrument. After calculation by the formula, 2^-∆∆Ct is obtained. We calculated the relative expression of the target gene according to the 2^-∆∆Ct. We selected EF-1a and β-actin as internal reference genes.

Ct: The number of threshold cycles, the number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold.

2-^∆∆Ct: The gene expression level change of the treatment group relative to the control group.

The naming of different treatments is as follows: The dsGFP and dsUGT344J7 represent dsRNA injected into aphids; 24h/48h/72h represents the time point after injection of dsRNA.

 

Processed_data_after_raw_data-Figure3-Mortality-of-R._padi-under-different-treatments.csv

Raw_data_for-Figure3-Mortality-of-R._padi-under-different-treatments.csv

Data brief description: In Figure 3, we examined the mortality of aphids after temperature treatment and starvation treatment. At the same time, the mortality of aphids treated with temperature and starvation after interference with UGT344J7 was also detected. It should be noted that there is a case where the number of deaths is 0 in some treatments. In this case, the mortality and sine conversion data are not available. We sinusoidally convert the mortality rate and then perform a significance analysis.

0: Under different temperature treatments or starvation treatments, the number of aphid deaths was 0.

NA: Because the number of deaths is 0, it is not possible to calculate.

The naming of different treatments is as follows:

C represents the control group (It is worth noting that we made corresponding controls at different temperatures and different starvation treatments. In the file to C1, C2 to distinguish. C1 represents the control group under temperature treatment, and C2 represents the control group under starvation treatment. The dsGFP and dsUGT344J7 represent dsRNA injected into aphids

In the experiment of temperature treatments, 36 and 38 represent the treatment temperature of 36℃ and 38℃, respectively; 2h and 4h represent temperature treatment time;

In the experiment of starvation treatment, S12, S24, S36 and S48 represent hunger for 12hours, 24 hours 36 hours and 48 hours, respectively.

 

Processed_data_after_raw_data-Figure4-Relative-expression-levels-of-Hsp-genes.xlsx

Raw_data_for-Figure4-Relative-expression-levels-of-Hsp-genes.xlsx

Data brief description: In figure 4, we detected the relative expression of hsp70-1, hsp70-2, hsp68 and hsp90 after high temperature treatment and the relative expression of hsp70-1, hsp70-2, hsp68 and hsp90 after interfering with UGT344J7. The detection method used was RT-qPCR. Each treatment had three mechanical and three biological replicates. The ct value is the original data detected by the instrument. After calculation by the formula, 2^-∆∆Ct is obtained. We calculated the relative expression of the target gene according to the 2^-∆∆Ct. We selected EF-1a and β-actin as internal reference genes.

Ct: The number of threshold cycles, the number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold.

2^-∆∆Ct: The gene expression level change of the treatment group relative to the control group.

The naming of different treatments is as follows:

C represents the control group (Note: For the treatment of different genes at different temperatures, we set up corresponding control groups. In the file to C1, C2, C3, C4, C5, C6, C7, C8 to distinguish. (C1 represents the control group of the hsp70-1 gene at 36℃, C2 represents the control group of the hsp70-2 gene at 36℃, C3 represents the control group of the hsp68 gene at 36℃, C4 represents the control group of the hsp90 gene at 36℃, C5 represents the control group of the hsp70-1 gene at 38℃, C6 represents the control group of the hsp70-2 gene at 38℃, C7 represents the control group of the hsp68 gene at 38℃, C8 represents the control group of the hsp90 gene at 38℃)

H36 and H38 represent the treatment temperature of 36℃ and 38℃, respectively; 2h and 4h represent temperature treatment time.

The dsGFP and dsUGT344J7 represent dsRNA injected into aphids.

hsp70-1, hsp70-2, hsp68, and hsp90 represent the detected genes, respectively.
 

 

Processed_data_after_raw_data-Figure5-The-ROS-contents.xlsx

Raw_data_for-Figure5-The-ROS-contents.csv

Data brief description: In Fig.5, we examined the effects of high temperature treatment, starvation treatment, and interference with UGT344J7 on the content of reactive oxygen species in aphids. The raw data shows the absorbance detected by the instrument (at 450 nm), which is converted into the active oxygen content in the processed data by the standard curve. We finally analyzed ROS content (ng/ml). Three biological replicates were set for each treatment.

The naming of different treatments is as follows:

C represents the control group (Note: For the treatment of different genes at different temperatures, we set up corresponding control groups. In the file to C1, C2, C3 to distinguish. C1 was the control group treated with 36℃ and 38℃ for 2 hours, C2 was the control group treated with 36℃ and 38℃ for 4 hours, C3 was the control group treated with starvation for 12 hours, 24 hours, 36 hours and 48 hours.

H36 and H38 represent the treatment temperature of 36℃ and 38℃, respectively; 2h and 4h represent temperature treatment time.

The dsGFP and dsUGT344J7 represent dsRNA injected into aphids.

S12, S24, S36 and S48 represent hunger for 12hours, 24 hours 36 houers and 48 hours, respectively;

 

FigureS1-The-relative-expression-of-43-UGT-genes-after-prolonged-high-temperature-treatment.csv

Data brief description: The expression changes of 43 UGT genes in aphids after high temperature treatment were shown in S1. Three biological replicates were set for each treatment. The data in the table represent the relative expression of different genes under different treatments, which is 2^-∆∆Ct value. The value of 2^-∆∆Ct has no unit, which is a relative multiple. By analyzing the data of the control group and the high temperature treatment group, we can find that the expression of UGT344J7 gene is significantly higher than that of other genes.

The naming of different treatments is as follows:

Name: The names of 43 UGT genes in R.padi.

CK: Normal feeding temperature treatment.

GW: high temperature treatment.

 

Processed_data_after_raw_data-FigureS2-Mortality-under-normal-conditions-following-injection-of-dsRNA.csv

Raw_data_for-FigureS2-Mortality-under-normal-conditions-following-injection-of-dsRNA.csv

Data brief description: In S2, we examined the effect of interfering with UGT344J7 on the mortality of R.padi. there is a case where the number of deaths is 0 in some treatments. In this case, the mortality and sine conversion data are not available. We sinusoidally convert the mortality rate and then perform a significance analysis. Three biological replicates were set for each treatment.

0: Under different temperature treatments or starvation treatments, the number of aphid deaths was 0.

NA: Because the number of deaths is 0, it is not possible to calculate.

The naming of different treatments is as follows:

The dsGFP and dsUGT344J7 represent dsRNA injected into aphids; 24h/48h/72h represents the time point after injection of dsRNA.