The physiological changes and the impact of rapid cold hardening on the survival ability of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) under cold stress
Data files
Feb 20, 2025 version files 8.62 KB
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Enzyme_activity.csv
2.94 KB
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README.md
2.88 KB
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Super_cooling_point_data_.csv
97 B
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Survival_rate_under_cold_stress_data.csv
2.19 KB
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Total_fat_and_water_content_data.csv
520 B
Abstract
Dastarcus helophoroides (Fairmaire) serves as an ideal biological control agent for forest beetles. However, its survival ability at low temperatures is a critical factor for its use in colder regions. This study investigates the survival ability, supercooling point (SCP), impact of rapid cold hardening (RCH), and physiological changes of D. helophoroides under cold stress. SCPs of adults that underwent RCH were lower than those of adults cultured at room temperature, with SCPs of -25.1°C and -16.9°C, respectively. As the cold stress temperature decreased in the test, the survival ability of D. helophoroides adults also diminished, reaching the lowest survival rates at a -20°C across all observation time points in the test. However, adults induced by RCH exhibited higher survival rates than those cultured at room temperature, suggesting that RCH enhances the cold resistance of D. helophoroides. The activities of superoxide dismutase (SOD) and catalase (CAT) in the adults increased as the stress temperature decreased. With prolonged cold stress, the activity of lactate dehydrogenase (LDH) initially decreased and then increased. Both fat and water content decreased with the lowering of stress temperature. These results provide insights into the cold resistance strategies of D. helophoroides.
https://doi.org/10.5061/dryad.0k6djhbbb
Description of the data and file structure
The survival rate, SOD activity, CAT activity, LDH activity, total fat, and water content data of Dastarcus helophoroides under cold stress were collected.
Files and variables
File: Enzyme_activity.csv
Description: This data file includes the activities of superoxide dismutase (SOD), catalase (CAT) and lactate dehydrogenase (LDH) of Dastarcus helophoroides under cold stress.
Variables
- Temperature: Experimental samples treated temperature ( 0℃, -5℃, -10℃, -15℃, and -20℃).
- Group number: 3 groups were tested for each temperature and processing day.
- Processing days: Experimental samples treated days (1, 3, 5, 7, and 9 days).
- SOD activity (mg pro/ml): superoxide dismutase activity (mg pro/ml).
- CAT activity (U/ml): catalase activity (U/ml).
- LDH activity (U/ml): lactate dehydrogenase activity (U/ml).
File: Survival_rate_under_cold_stress_data.csv
Description: This data file includes the survival rate of regular D. helophoroides adults and rapid cold hardening D. helophoroides adults under cold stress.
Variables
- Temperature: Experimental samples treated temperature (25℃, 0℃, -5℃, -10℃, -15℃, and -20℃).
- Group number: 3 groups were tested for each temperature and processing day.
- Processing days: Experimental samples treated days (1, 3, 5, 7, and 9 days).
- RD survival rate (%): regular D. helophoroides survival rate (%)
- RCHD survival rate (%): rapid cold hardening D. helophoroides survival rate (%)
File: Super_cooling_point_data_.csv
Description: This data file includes the super cooling points of regular D. helophoroides adults and rapid cold hardening treated D. helophoroides adults.
Variables
- Group number: 5 groups were tested for super cooling point
- RD SCP (℃): regular D. helophoroides super cooling point
- RCHD SCP (℃): rapid cold hardening treated D. helophoroides super cooling point
File: Total_fat_and_water_content_data.csv
Description: This data file includes the total fat and water content of D. helophoroides adults under cold stress.
Variables
- Temperature: Experimental samples treated temperature (25℃, 5℃, 0℃, -5℃, -10℃, -15℃, and -20℃).
- Group number: 3 groups were tested for each temperature.
- Processing days: Each sample were processing for 2 days.
- Total fat (%): The total fat of D. helophoroides adults under cold stress.
- Water content (%): The water content of D. helophoroides adults under cold stress.