Biomass yields, reproductive fertility, compositional analysis and genetic diversity of newly developed triploid giant Miscanthus Hybrids
Data files
May 22, 2024 version files 267.09 GB
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1993-1780.zip
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2004-256.zip
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2004-266.zip
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6x_giganteus.zip
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ADF.csv
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Biochemical_Table4.csv
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biomass_ha.csv
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Crude_Protein.csv
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CulmNumber_CulmHeight_FoliageHeight.csv
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fastq.pdf
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Fertility.csv
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Figure_01_data.csv
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Genome_Size.csv
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Genomic_Size_distribution_raw.csv
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H2014-156-003.zip
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H2014-156-007.zip
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H2014-156-011.zip
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H2015-004-007.zip
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H2015-004-012.zip
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H2015-004-014.zip
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H2015-004-019.zip
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H2015-004-038.zip
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H2015-004-044.zip
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H2015-004-049.zip
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H2015-004-050.zip
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H2015-004-069.zip
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H2015-004-074.zip
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H2015-004-079.zip
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H2015-004-087.zip
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H2015-004-096.zip
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H2015-004-102.zip
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H2015-007-013.zip
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KAVL_30Year_Means.csv
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KAVL_Precipitation_Data_2017-2020.csv
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KAVL_Temperature_Data_2017-2020.csv
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Mgiganteus_Freedom.zip
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NDF.csv
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NFC.csv
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Peak_Anthesis_date.csv
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Pollen_Viablility.csv
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README_for_1993-1780.zip.txt
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README_for_2004-256.zip.txt
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README_for_2004-266.zip.txt
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README_for_6x_giganteus.zip.txt
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README_for_fastq.pdf.txt
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README_for_Figure_01.txt
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README_for_Figure_02.txt
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README_for_Figure_03_Table_06.txt
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README_for_H2014-156-003.zip.txt
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README_for_H2014-156-007.zip.txt
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README_for_H2014-156-011.zip.txt
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README_for_H2015-004-007.zip.txt
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README_for_H2015-004-012.zip.txt
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README_for_H2015-004-014.zip.txt
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README_for_H2015-004-019.zip.txt
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README_for_H2015-004-038.zip.txt
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README_for_H2015-004-044.zip.txt
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README_for_H2015-004-049.zip.txt
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README_for_H2015-004-050.zip.txt
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README_for_H2015-004-069.zip.txt
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README_for_H2015-004-074.zip.txt
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README_for_H2015-004-079.zip.txt
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README_for_H2015-004-087.zip.txt
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README_for_H2015-004-096.zip.txt
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README_for_H2015-004-102.zip.txt
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README_for_H2015-007-013.zip.txt
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README_for_Mgiganteus_Freedom.zip.txt
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README_for_table_01.txt
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README_for_table_02.txt
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README_for_table_03.txt
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README_for_table_04.txt
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README_for_table_05.txt
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README.md
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RFV.csv
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Theoretical_Ethanol.csv
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Abstract
Miscanthus ×giganteus (giant miscanthus) is a naturally occurring hybrid that has shown promise as a bioenergy/biomass crop throughout much of the temperate world. Allotriploid (2n = 3x = 57) hybrids of M. ×giganteus, resulting from crosses between tetraploid M. sacchariflorus (2n = 4x = 76) and diploid M. sinensis (2n = 2x = 38), are particularly desirable due to their low fertility that minimizes reseeding and potential invasiveness. However, there is limited genetic diversity in commonly grown cultivars of triploid M. ×giganteus and breeding and development efforts to improve and domesticate this crop have been minimal. Here, we report on newly developed M. ×giganteus hybrids compared to the industry standard M. ×giganteus ‘1993-1780’. Dry biomass yields of new hybrids ranged from 19.5 to 32.4 Mg/ha per year for the fourth growing season, compared to 21.0 Mg·ha−1 for M. ×giganteus ‘1993-1780’. Plant reproductive fertility remained low for all accessions with overall fertility [(seed set · seed germination)/100] ranging from 0.3 to 4.5% for new hybrids compared to 0.4% for M. ×giganteus ‘1993-1780’. Culm density and height varied among accessions and were positively correlated with increased biomass. Based on compositional analyses, theoretical ethanol yields ranged from 9,740 to 16,278 L/ha for new hybrids compared to 10,406 L/ha for M. ×giganteus ‘1993-1780’. Relative feed value (RFV) indices were low overall and ranged between 66.0 and 72.8 for new hybrids compared to M. ×giganteus ‘1993-1780’ with 71.3. The genetic diversity of new hybrids, compared to existing cultivars, was characterized using whole genome sequences. Based on pair-wise distances, cluster analysis clearly showed increased diversity of new hybrids compared to earlier selections. These results document new triploid hybrids of M. ×giganteus with enhanced biomass and theoretical ethanol yields in combination with broader genetic diversity and low reproductive fertility.
https://doi.org/10.5061/dryad.1c59zw445
Included is the data used for Figures 1, 2, and 3 and Tables 1, 2, 3, 4, 5 and 6 in the manuscript.
Figure 3 and Table 6 are based upon the same DNA sequence data.
Description of the data and file structure
Figure_01_data.csv: Yield data in Megagrams per Hectare (Mg/ha) for the years 2017 through 2020 for each accession.
Genomic_Size_distribution_raw.csv: Flow cytometry genomic size estimates in picograms (pg) for Figure 2.
Sequence Data for Figure 3 and Table 6.
Whole genome sequence data generated by Novogene Co., Inc. (Beijing, China). Standard Illumina DNA libraries were prepared with an insert size of 350 bp and then sequenced using an Illumina HiSeq instrument (Illumina, San Diego, CA) producing 150-bp paired-end sequencing reads with an average 25 Gb of raw data per sample.
FastQ sequence filetype (.fq) is compressed with gzip (.gz) and then stored in a ZIP file (.zip) with relative path structure. The relative path structure is a directory labeled with the accession identifier, as indicated in the manuscript, containing three files. There are two fastQ DNA sequence files, one for each direction of the sequence read, labeled with the accession identifier followed by an underscore and a number 1 or number 2, ie 6x_giganteus_1.fq.gz or 1993-1780_2.fq.gz, and the original MD5 hash text file that can be used to check file integrity after download.
The file fastq.pdf, included with the dataset, provides additional general information about the fastq file type.
File and directory structure:
6x_giganteus.zip
- 6x_giganteus/
- 6x_giganteus_1.fq.gz
- 6x_giganteus_2.fq.gz
- MD5.txt
1993-1780.zip
- 1993-1780/
- 1993-1780_1.fq.gz
- 1993-1780_2.fq.gz
- MD5.txt
2004-256.zip
- 2004-256/
- 2004-256_1.fq.gz
- 2004-256_2.fq.gz
- MD5.txt
2004-266.zip
- 2004-266/
- 2004-266_1.fq.gz
- 2004-266_2.fq.gz
- MD5.txt
H2014-156-003.zip
- H2014-156-003/
- H2014-156-003_1.fq.gz
- H2014-156-003_2.fq.gz
- MD5.txt
H2014-156-007.zip
- H2014-156-007/
- H2014-156-007_1.fq.gz
- H2014-156-007_2.fq.gz
- MD5.txt
H2014-156-011.zip
- H2014-156-011/
- H2014-156-011_1.fq.gz
- H2014-156-011_2.fq.gz
- MD5.txt
H2015-004-007.zip
- H2015-004-007/
- H2015-004-007_1.fq.gz
- H2015-004-007_2.fq.gz
- MD5.txt
H2015-004-012.zip
- H2015-004-012/
- H2015-004-012_1.fq.gz
- H2015-004-012_2.fq.gz
- MD5.txt
H2015-004-014.zip
- H2015-004-014/
- H2015-004-014_1.fq.gz
- H2015-004-014_2.fq.gz
- MD5.txt
H2015-004-019.zip
- H2015-004-019/
- H2015-004-019_1.fq.gz
- H2015-004-019_2.fq.gz
- MD5.txt
H2015-004-038.zip
- H2015-004-038/
- H2015-004-038_1.fq.gz
- H2015-004-038_2.fq.gz
- MD5.txt
H2015-004-044.zip
- H2015-004-044/
- H2015-004-044_1.fq.gz
- H2015-004-044_2.fq.gz
- MD5.txt
H2015-004-049.zip
- H2015-004-049/
- H2015-004-049_1.fq.gz
- H2015-004-049_2.fq.gz
- MD5.txt
H2015-004-050.zip
- H2015-004-050/
- H2015-004-050_1.fq.gz
- H2015-004-050_2.fq.gz
- MD5.txt
H2015-004-069.zip
- H2015-004-069/
- H2015-004-069_1.fq.gz
- H2015-004-069_2.fq.gz
- MD5.txt
H2015-004-074.zip
- H2015-004-074/
- H2015-004-074_1.fq.gz
- H2015-004-074_2.fq.gz
- MD5.txt
H2015-004-079.zip
- H2015-004-079/
- H2015-004-079_1.fq.gz
- H2015-004-079_2.fq.gz
- MD5.txt
H2015-004-087.zip
- H2015-004-087/
- H2015-004-087_1.fq.gz
- H2015-004-087_2.fq.gz
- MD5.txt
H2015-004-096.zip
- H2015-004-096/
- H2015-004-096_1.fq.gz
- H2015-004-096_2.fq.gz
- MD5.txt
H2015-004-102.zip
- H2015-004-102/
- H2015-004-102_1.fq.gz
- H2015-004-102_2.fq.gz
- MD5.txt
H2015-007-013.zip
- H2015-007-013/
- H2015-007-013_1.fq.gz
- H2015-007-013_2.fq.gz
- MD5.txt
Mgiganteus_Freedom.zip
- Mgiganteus_Freedom/
- MD5.txt
- Mgiganteus_Freedom_1.fq.gz
- Mgiganteus_Freedom_2.fq.gz
Data for Table 1
KAVL_30Year_Means.csv: Monthly precipitation and temperature means for 30 years, 1991 through 2020.
KAVL_Precipitation_Data_2017-2020.csv: Monthly Precipitation for the years 2017 through 2020.
KAVL_Temperature_Data_2017-2020.csv: Monthly temperature data for the years 2017 through 2020.
Data for Table 2
Fertility.csv: Percent Seed set, Percent Seed germination, Percent Female Fertility percent.
Genome_Size.csv: Genome size in pg.
Pollen_Viablility.csv: Percent pollen viability dataset.
Data for Table 3
CulmNumber_CulmHeight_FoliageHeight.csv: Culm Number and Culm Height in meters and Foliage height in meters collected November 2020.
Peak_Anthesis_date.csv: Peak anthesis, Day of the Year number for 2020.
Data for Table 4
Biochemical_Table4.csv: Biochemical values for Table 4, Acid insoluable lignin (grams AIL/grams of sample), Cellulose (grams cellulose/ grams of sample), Hemicellulose (grams hemicellulose/grams of sample), Glucose (grams glucose/grams sample), Fructose (grams fructose/grams sample).
biomass_ha.csv: Biomass in Mg/ha for 2017 through 2020.
Theoretical_Ethanol.csv: Theoretical ethanol yield Liters per Hectare (L/ha).
Data for Table 5
ADF.csv: Acid detergent fiber ADF(%). Reported in the table as (g/kg) therefore '1993-1780' adf 431.7 g/Kg = 431.7 g/1000g = 0.4317 g/g = 43.17%
Crude_Protein.csv: Crude Protein (%). Reported in the table as (g/kg) therefore '1993-1780' Crude Protein 79.5 g/Kg = 79.5 g/1000g = 0.0795 g/g = 7.95%
NDF.csv: Neutral Detergent Fiber NDF (%). Reported in the table as (g/kg) therefore '1993-1780' NDF 721.0 g/Kg = 721.0 g/1000g = 0.721 g/g = 72.1%
NFC.csv: Non-fiber carbohydrate NFC(%). Reported in the table as (g/kg) therefore '1993-1780' NFC 229.4 g/Kg = 229.4 g/1000g = 0.2294 g/g = 22.94%
RFV.csv: Relative Feed Value.
Sharing/Access information
Table 1 data set is obtained from the National Oceanic and Atmospheric Administration. (2023) U.S. Climate Normals, 1991-2020. November, 2023. http://www.ncdc.noaa.gov/cdo-web/datatools/normals and North Carolina State Climate Office. (2023). Cardinal data retrieval system, KAVL – Asheville Regional Airport. November. 2023. https://products.climate.ncsu.edu/cardinal/scout/?station=KAVL.
- Touchell, Darren H.; Lynch, Nathan; Shekasteband, Reza et al. (2024). Biomass yields, reproductive fertility, compositional analysis, and genetic diversity of newly developed triploid giant miscanthus hybrids. GCB Bioenergy. https://doi.org/10.1111/gcbb.13174
