Corn silk extract as a prebiotic exerts antihypertensive effects via gut microbiota modulation in hypertensive rats
Data files
Nov 19, 2025 version files 266.19 MB
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JA0405_1.338F_806R.R1.raw.fastq.gz
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JA0405_1.338F_806R.R2.raw.fastq.gz
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JA0413_4.338F_806R.R1.raw.fastq.gz
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JA0414_1.338F_806R.R1.raw.fastq.gz
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README.md
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Abstract
Corn silk extract (CSE), a traditional medicinal food rich in polysaccharides, flavonoids, and saponins, has been used as a natural antihypertensive agent, but its mechanism remains unclear. This study aimed to evaluate whether corn silk extract can lower blood pressure through gut microbiota modulation. Spontaneously hypertensive rats received oral corn silk extract for four weeks, followed by a four-week drug-free observation. The treatment significantly reduced systolic blood pressure, increased microbial diversity, decreased the Firmicutes/Bacteroidetes ratio, and enriched beneficial genera such as Akkermansia and Lactobacillus. These changes were accompanied by reduced serum LPS and pro-inflammatory cytokines, elevated NO levels, and restored endothelial function. PERMANOVA and correlation analyses showed that microbiota and inflammatory markers were more strongly associated with blood pressure improvements than urinary indices. Structural equation modeling suggested a potential mechanistic pathway involving gut microbiota–inflammation–NO regulation. Importantly, fecal microbiota transplantation (FMT) using post-treatment donor samples reproduced the antihypertensive and anti-inflammatory effects, confirming the microbiota’s critical mediating role. These findings provide the first experimental evidence that corn silk extract functions as a prebiotic to improve gut microbial balance and vascular health, offering a promising natural strategy for microbiota-targeted blood pressure control.
README: Corn silk extract as a prebiotic exerts antihypertensive effects via gut microbiota modulation in hypertensive rats
Dataset DOI: 10.5061/dryad.34tmpg4w9
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1. Overview of the dataset
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This dataset contains raw 16S rRNA gene sequencing data (paired-end FASTQ files) obtained from fecal samples of spontaneously hypertensive rats (SHR) enrolled in a controlled intervention study evaluating the antihypertensive and microbiota-modulating effects of corn silk extract (CSE). Fecal samples were stored at −80°C prior to DNA extraction【32:L9-L9】.
Genomic DNA was extracted using the QIAamp Fast DNA Stool Mini Kit (Qiagen)【32:L9-L9】. The V3–V4 region of the bacterial 16S rRNA gene was amplified using primers 338F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT)【32:L17-L17】. Sequencing was performed on the Illumina NovaSeq 6000 platform (PE250 mode)【2:L1-L1】.
All sequencing files included here are raw FASTQ outputs without trimming, filtering, or merging.
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2. File naming convention
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Each file follows the naming pattern:
SampleID.Batch.PrimerPair.ReadDirection.raw.fastq.gz
Definitions:
- SampleID: individual rat or sample identifier (e.g., JA0405, JA0412, JB18)
- Batch: sequencing batch or technical replicate number (e.g., 1, 2, 3)
- PrimerPair: always 338F_806R for this dataset
- ReadDirection: R1 = forward read, R2 = reverse read
- raw.fastq.gz: gzip-compressed unprocessed FASTQ file
Example:
JA0405_1.338F_806R.R1.raw.fastq.gz
→ Sample JA0405, batch 1, primer pair 338F/806R, forward read.
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3. File count
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- Total FASTQ files: 112
- Total biological samples: 56
- Each biological sample includes two paired-end reads (R1 and R2).
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4. Explanation of abbreviations
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- SHR = Spontaneously Hypertensive Rat
- CSE = Corn Silk Extract
- R1 / R2 = Forward and reverse reads in paired-end sequencing
- 338F / 806R = Primer pair for V3–V4 amplification
- FASTQ = Text-based sequence format containing nucleotide sequences + quality scores
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5. Sample processing and sequencing details (from manuscript)
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- Fecal samples were collected at the endpoint of the animal experiment and stored at −80°C immediately【32:L9-L9】.
- Microbial DNA extraction was conducted using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany)【32:L9-L9】.
- The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers
• 338F: ACTCCTACGGGAGGCAGCAG
• 806R: GGACTACHVGGGTWTCTAAT
(primer sequences from your manuscript【32:L17-L17】)
- PCR products were purified using AMPure XP magnetic beads (Beckman Coulter).
- Libraries were sequenced on the Illumina NovaSeq 6000 (PE250) platform【2:L1-L1】.
- Files in this dataset represent raw FASTQ reads prior to any quality control, trimming, or merging.
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6. Recommended downstream usage
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The raw data are suitable for:
- DADA2 or Deblur ASV inference
- QIIME2-based workflows
- Taxonomic annotation using SILVA v138 (as used in the manuscript)
- Alpha/beta diversity, PCoA, community composition, and functional prediction (PICRUSt2)
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7. Full file list
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JA0405_1.338F_806R.R2.raw.fastq.gz
JA0405_2.338F_806R.R1.raw.fastq.gz
JA0405_2.338F_806R.R2.raw.fastq.gz
JA0406_1.338F_806R.R1.raw.fastq.gz
JA0406_1.338F_806R.R2.raw.fastq.gz
JA0406_2.338F_806R.R1.raw.fastq.gz
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JA0412_2.338F_806R.R1.raw.fastq.gz
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JA0413_1.338F_806R.R1.raw.fastq.gz
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