Data from: Hedgehog pathway disruption alters urothelial differentiation and continence mechanisms in the female mouse lower urinary tract
Data files
Feb 19, 2026 version files 9.25 GB
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Adult_IHC_raw_data.xlsx
38.20 KB
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Bladder_bath_raw_data.xlsx
41.30 KB
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Body_and_tissue_mass_raw_data.xlsx
15.79 KB
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Craniofacial_E16_Morphemetrics_raw_data.xlsx
14.85 KB
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E16_IHC_raw_data.xlsx
13.60 KB
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e16_images.zip
5.17 GB
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Krt5_Krt13_images.zip
857.70 MB
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Krt5_Upk3a_images.zip
3.17 GB
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raw_vsa_images.zip
51.89 MB
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README.md
8.41 KB
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Urethra_bath_raw_data.xlsx
24.11 KB
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Urethra_qPCR_raw_data.xlsx
18.32 KB
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Uroflow_raw_data.xlsx
18.67 KB
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VSA_raw_data.xlsx
10.70 KB
Abstract
Urinary incontinence (UI) imposes a significant healthcare burden and reduces quality of life. Contributing factors such as aging, pregnancy/childbirth, stress, and injury are recognized, but an incomplete understanding of underlying mechanisms limits new therapies. Hedgehog (Hh) signaling has been implicated in lower urinary tract development, but its specific role in female continence mechanisms has not been fully characterized. Here, we investigate the functional and molecular consequences of reduced Hh signaling using Gli2+/-;Gli3Δ699/+, (Gli mutant) female mice. We assessed spontaneous voiding through void spot assays and uroflowmetry, then assessed contractility in bladder and urethral tissues ex vivo. Female Gli mutant mice display more small-volume voids than wild-type mice. Gli mutant female bladders had reduced contractile strength to electrical field and cholinergic stimuli, while the urethra had reduced sensitivity to serotonin-mediated contraction, but not to phenylephrine. Thus, unique changes to bladder and urethral contractility dynamics are present in Gli mutant mice and are dependent upon the type of stimuli. Further, expression of serotonin transporter (Sert) mRNA was increased in the Gli mutant urethra compared to the wild type. Uroplakin IIIa, typically localized to bladder urothelium, was ectopically expressed in urethral urothelium of adult but not embryonic (E) day 16 Gli mutant mice. These findings highlight a previously uncharacterized role of Hh signaling in maintaining female lower urinary tract function and urothelial patterning, and support further investigation of its contribution to continence.
Dataset DOI: 10.5061/dryad.3n5tb2rxg
Description of the data and file structure
Excel files of raw data for physiology and histology assessments in wild-type and Gli mutant female mice.
Files and variables
File: VSA_raw_data.xlsx
Description: Raw data generated from running void spot assays on wild-type and mutant animals. After the voiding assay, the filter papers containing urine were imaged with UV light,t and the resulting images were analyzed via Void Whizzard software freely available as a plugin in Image J. This Excel table is the readout of the Void Whizzard analysis of the urine content on the assay papers. The first column is the animal's unique ID, the second column is the genotype of that animal the third column is the name of the void paper image file. Each column after that is the voiding parameter examined, and each row is a different animal.
Variables
- The first column is the animal's unique ID, the second column is the genotype of that animal the third column is the name of the void paper image file. Each column after that is the voiding parameter examined, and each row is a different animal.
File: Uroflow_raw_data.xlsx
Description: Raw data generated from running uroflowmetry on wild-type and mutant animals. After the assay, the videos captured by Raspberry Pi units during uroflowmetry testing were analyzed. ThisExcell table is the analysis of each captured void in the mass and time of each void. The first column is the animal's unique ID, and the second column is the genotype of that animal. Each column header is a parameter examined, and each row is a void event for each different animal. Columns U through AB are the averages of all parameters from all voids from a single animal.
Variables
- The first column is theanimal'sl unique ID, the second column is the genotype of that animal. All column headers describe the variable assessed; each row is a void event split by each individual animal. Column U through AB are the averages of all parameters from all voids from a single animal.
File: Bladder_bath_raw_data.xlsx
Description: Raw data generated from bladder contractility assays on wild-type and mutant animals using LabChart software during testing. Each column header is a parameter examined, and each row is a different bladder separated for each animal. Two bladder halves were averaged to have one end value for each parameter from each animal.
Variables
- The first column is the animal's unique ID, and the second column is the genotype of that animal. All other columns are parameters measured. Row 1 names the type of analysis. Row 4 and 5 indicate which treatment was applied, columns E-AD are the raw values of the parameter. All subsequent columns are calculations of the raw data in E-AD, as stated in Row 1 for that column.
File: Urethra_bath_raw_data.xlsx
Description: Raw data generated from urethral contractility assays on wild-type and mutant animals using LabChart software during wire myography testing. Each column header is a parameter examined, and each row is a different animal.
Variables
- The first column is theanimal'sl unique ID, the second column is the sex, and the third column is the genotype of that animal. All other columns are parameters measured. Row 1 names the type of analysis. Row 2 and 3 indicate which treatment was applied, columns D-AK are the raw values of the parameter. All subsequent columns are calculations off of the raw data in D-AK, and as stated in Row 1 for that column.
File: Urethra_qPCR_raw_data.xlsx
Description: Raw data generated from qPCR analysis of the urethra from wild-type and mutant mice. Each column header is a gene examined, and each row is a different animal. Raw Ct values are presented,d and the delta Ct calculation is presented.
Variables
- The first column is the animal's unique ID, the second column is the genotype of that animal, third column is sex. All other columns are parameters measured. Columns D-J are raw Ct values. Column P-U is the calculation of the delta Ct using housekeeping gene Ppia for relative expression.
File: Adult_IHC_raw_data.xlsx
Description: Raw data generated from quantifying the percentage of total positive cells from images. Each column header is an endpoint examined, and each row is a different image, separated out for each animal.
Variables
- All column headers describe the protein marker expressed, the cell count, or average; each row is an image, which is separated out for each animal. Each tab is a set of slides and stains used for cell counting. The summary tab is the average from all of those stains, and finally averaged for a single number for each animal.
File: E16_IHC_raw_data.xlsx
Description: Raw data generated from quantifyingthe percent total positive cells from images. Each column header is an endpoint examined, and each row is a different image, separated out for each animal.
Variables
- All column headers describe the protein marker expressed, cell count, or average, each row is an image, which is separated out for each animal.
File: Body_and_tissue_mass_raw_data.xlsx
Description: Raw data generated from all animals used in the study. Each column header is an endpoint examined, and each row is a different animal.
Variables
- All column headers describe the endpoint assessed; each row is a different animal.
File: Craniofacial_E16_Morphemetrics_raw_data.xlsx
Description: Raw data generated from all animals used in the study. Each column header is an endpoint examined, and each row is a different animal.
Variables
- All column headers describe the endpoint assessed; each row is a different animal.
File: raw_vsa_images.zip
Description: Images of papers from void spot assay (VSA) imaged under UV light from all animals used in the study.
Variables
- All file names are the animal ID. All are Tiffs of the imaged paper that was placed in the cage for the mouse to urinate on for 4 hours.
File: e16_images.zip
Description: Fluorescent images from embryonic day (E16) urogenital sinus tissue sections generated from all animals used in the study, which were stained via immunohistochemistry with antibodies targeting keratin 5 (red) and uroplakin IIIa (green), DAPI (nuclei, blue). All files are the merged red, green, and blue channels as a TIFF.
Variables
- All file names describe the endpoint assessed and animal ID. File names are the block number, animal number, and a description of the antibodies used and their color, along with where in the urogenital sinus the image was taken.
File: Krt5_Krt13_images.zip
Description: Fluorescent images from bladder and urethra tissue sections generated from all animals used in the study, which were stained via immunohistochemistry with antibodies targeting keratin 5 (red) and keratin 13 (green), DAPI (nuclei, blue). All files are the merged red, green, and blue channels as a TIFF.
Variables
- All file names describe the endpoint assessed and animal ID. File names are the block number, animal number,r and a description of the antibodies used and their color, along with where in the tissue the image was taken.
File: Krt5_Upk3a_images.zip
Description: Fluorescent images from bladder and urethra tissue sections generated from all animals used in the study, which were stained via immunohistochemistry with antibodies targeting keratin 5 (red) and uroplakin IIIa (green), DAPI (nuclei, blue). All files are the merged red, green, and blue channels as a TIFF.
Variables
- All file names describe the endpoint assessed and animal ID. File names are the block number, animal number, and a description of the antibodies used and their color,r along with where in the tissue the image was taken.
Code/Software
All data can be viewedin Excell or a photo viewer.
Void Whizzard software (free plugin for Image J) - was used for VSA analysis
Access information
Other publicly accessible locations of the data:
- None
Data was derived from the following sources:
- None