Data from: Germination of 10 midland plant species from the eastern Mediterranean Basin: Effects of smoke, syringaldehyde, karrikinolide, and cyanohydrin
Data files
Jul 17, 2024 version files 15.29 KB
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Germination_data_sal.txt
4.98 KB
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Germination_data_smoke_chemicals.txt
3.24 KB
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Germination_data_smoke.txt
3.86 KB
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README.md
2.15 KB
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seed_mass_data.txt
1.06 KB
Abstract
The beneficial effects of smoke and its constituents, karrikinolide (KAR1) and cyanohydrin glyceronitrile, on the germination of Mediterranean lowland species are well-documented. However, very little is known about the role of these signals on the germination of plants at higher altitudes. In addition, lignin-derived chemicals, such as syringaldehyde (SAL), have recently been proposed as overlooked cues for smoke-induced germination. To address these gaps in the literature, we investigated the effects of smoke-water and SAL on the germination of 10 midland species growing on serpentine soils. We also sought to determine whether SAL interacts with KAR1 and/or mandelonitrile (MAN, a cyanohydrin) to enhance seed germination. The results show that smoke-water significantly improved the germination of three species (Barbarea duralii, Digitalis cariensis, and Turritis laxa). SAL, on the other hand, had no positive effect on the germination of the 10 species tested. Furthermore, three smoke-sensitive species and Verbascum cariense responded to KAR1 and/or MAN. Finally, SAL did not exert any synergistic effects on germination in interaction with KAR1 and MAN. In conclusion, we provide evidence that smoke is an important germination cue also for Mediterranean midland species. Moreover, SAL did not play a role in stimulating germination in smoke-sensitive species, either independently or in combination with other smoke chemicals.
https://doi.org/10.5061/dryad.3tx95x6qh
Description of the data and file structure
The datasets are associated with three germination experiments and the mean seed mass of 10 species studied. The datasets are organized into four files in .txt format.
The first data file (Germination_data_smoke) contains three replicates of germination percentages for each smoke-water treatment per species. The treatment codes represent the concentration of each smoke-water (SW0 = 0% (v/v) smoke-water; SW1 = 1% (v/v) smoke-water; SW5 = 5% (v/v) smoke-water; SW10 = 10% (v/v) smoke-water).
The second data file (Germination_data_sal) contains three replicates of germination percentages for each syringaldehyde (SAL, a compound found in smoke-water) treatment per species. The treatment codes represent the concentration of each syringaldehyde solution (SAL0 = 0 ng µl−1 (w/v) SAL; SAL1 = 1 ng µl−1 (w/v) SAL; SAL2 = 2 ng µl−1 (w/v) SAL; SAL5 = 5 ng µl−1 (w/v) SAL; SAL10 = 10 ng µl−1 (w/v) SAL).
The third data file (Germination_data_smoke_chemicals) contains three replicates of germination percentages for single and combined applications of three smoke chemicals. The codes and concentrations of smoke chemicals are as follows: Syringaldehyde [SAL, 2 ng µl−1 (w/v)], Karrikinolide [KAR, 0.1 µM], and Mandelonitrile [a cyanohydrin, MAN, 50 µM]. In the first and second datasets, treatments were applied to 10 species studied. In the third dataset treatments were applied to 4 selected species. In all germination datasets, Pgerm represents "percent germination".
Finally, the fourth data file (seed_mass_data) contains three replicates of "the seed mass of 100 seeds" measured in grams, and "the mean seed mass (mg)" calculated from the first parameter for 10 species studied.
Code/Software
The code used to analyze the data using R software will be made available to any interested researcher upon request to the corresponding author.
- Çatav, Şükrü Serter et al. (2024), Germination of 10 midland plant species from the eastern Mediterranean Basin: effects of smoke, syringaldehyde, karrikinolide and cyanohydrin, Nordic Journal of Botany, Journal-article, https://doi.org/10.1111/njb.04457