Data and code from: Ingestion of the potent neurotoxin epibatidine does not compromise locomotion or behavior in the poison frog Epipedobates tricolor
Data files
Jun 17, 2026 version files 10.98 GB
-
1_h_assays.zip
2.24 GB
-
20260610_R_script_performance_assays.R
5.27 KB
-
24_h_assays.zip
2.18 GB
-
3_min_assays.zip
2.90 GB
-
4_h_assays.zip
1.97 GB
-
csv_files.zip
758.49 KB
-
Mouth_gaping.zip
1.69 GB
-
README.md
4.87 KB
Abstract
The Phantasmal poison frog, Epipedobates tricolor, has amino acid substitutions in its neuronal nicotinic acetylcholine receptors that were previously shown to provide resistance to the highly potent neurotoxin epibatidine in vitro. However, it is unclear whether E. tricolor resists the physiological effects of epibatidine in vivo. To investigate this, we examined the effects of epibatidine exposure on the locomotion and behavior of E. tricolor. We performed whole-animal performance assays and behavioral evaluations at multiple time points following the administration of high yet biologically relevant levels of epibatidine. This data set includes all the videos from the mouth-gaping analysis, whole-animal performance assays, the output from the recording analysis generated by imageJ, and the R script used to analyze and plot all data.
Dataset DOI: 10.5061/dryad.41ns1rnwm
Description of the data and file structure
Data from the whole-animal performance assays and behavioral evaluations performed at multiple time points following the administration of treatment solutions: water control, ethanol control, 2 µg epibatidine treatment, and 6 µg epibatidine treatment.
Individual frogs were assigned to each treatment as follows:
| Sample | Treatment |
|---|---|
| 27A | water_control |
| 27C | water_control |
| 38A | water_control |
| 60B | water_control |
| 71A | water_control |
| Locom9_A | ethanol_control |
| Locom9_B | ethanol_control |
| Locom9_C | ethanol_control |
| Locom10_A | ethanol_control |
| Locom10_B | ethanol_control |
| Locom10_C | ethanol_control |
| Locom11_A | ethanol_control |
| Locom11_B | ethanol_control |
| 27B | 2ug_treatment |
| 38B | 2ug_treatment |
| 53A | 2ug_treatment |
| 60A | 2ug_treatment |
| 71C | 2ug_treatment |
| Locom1_C | 2ug_treatment |
| Locom2_C | 2ug_treatment |
| Locom5_A | 2ug_treatment |
| Locom7_C | 2ug_treatment |
| Locom8_A | 2ug_treatment |
| 38C | 6ug_treatment |
| 53B | 6ug_treatment |
| 53C | 6ug_treatment |
| 60C | 6ug_treatment |
| 71B | 6ug_treatment |
| Locom1_A | 6ug_treatment |
| Locom2_B | 6ug_treatment |
| Locom4_A | 6ug_treatment |
| Locom6_A | 6ug_treatment |
| Locom6_B | 6ug_treatment |
Files and variables
File: csv_files.zip
Description: Output from image stack analysis using FFmeg version 6.0 and Fiji version 2.17.0
- File names are written as Sample ID followed by the time point when the whole-body performance assay was recorded.
- Example: sampleID_timepoint.cvs
- See manuscript for more details about the methods.
Columns description:
- Track n°: track number (not used in analysis)
- Slice n°: number of the frame
- X and Y: frog's coordinates in pixels
- Distance: distance traveled in centimeters
- Velocity: velocity (cm/s) traveled
- Pixel Value: brightness of the selected pixel (not used in analysis)
File: 20260610_R_script_performance_assays.R
Description: Custom R script used to analyze .csv output files generated by Fiji version 2.17.0.
- See manuscript for more details.
File: Mouth_gaping.zip
Description: Original video files (.mov) recorded immediately before and after the solution administration.
- File names include the individual species ID number, and either "before" or "after" according to when it was recorded after solution administration.
- Example: sampleID_before.MOV or sampleID_after.MOV
- There are 66 videos corresponding to 33 frogs from the different treatment groups.
File: 3_min_assays.zip
Description: Original video files (.mov) of whole-animal performance assays performed 3 minutes after solution administration.
- File names include the individual species ID number, and the time point when it was recorded.
- There are 33 videos corresponding to 33 frogs from the different treatment groups.
File: 1_h_assays.zip
Description: Original video files (.mov) of whole-animal performance assays performed 1 hour after solution administration.
- File names include the individual species ID number, and the time point when it was recorded.
- There are 33 videos corresponding to 33 frogs from the different treatment groups.
File: 4_h_assays.zip
Description: Original video files (.mov) of whole-animal performance assays performed 4 hours after solution administration.
- File names include the individual species ID number, and the time point when it was recorded.
- There are 32 videos corresponding to 32 frogs from the different treatment groups.
File: 24_h_assays.zip
Description: Original video files (.mov) of whole-animal performance assays performed 24 hours after solution administration.
- File names include the individual species ID number, and the time point when it was recorded.
- There are 25 videos corresponding to 25 frogs from the different treatment groups.
Code/software
- Videos: all videos are in .mov format which can be opened with most media player softwares.
- csv files: can be opened with any text editor.
- R script: can be opened with any text editor or using R software (https://www.r-project.org/).
Access information
Other publicly accessible locations of the data:
- NA
Data was derived from the following sources: - NA