Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 1
Data files
Jun 26, 2025 version files 55.41 GB
-
D0bos_msmegribgcomp_5_S23_L002_R1_001.fastq.gz
2.25 GB
-
D0bos_msmegribgcomp_5_S23_L002_R2_001.fastq.gz
2.23 GB
-
Dobos_msmegriba2comp_7_S25_L002_R1_001.fastq.gz
2.66 GB
-
Dobos_msmegriba2comp_7_S25_L002_R2_001.fastq.gz
2.68 GB
-
Dobos_msmegribccomp_1_S19_L002_R1_001.fastq.gz
2.50 GB
-
Dobos_msmegribccomp_1_S19_L002_R2_001.fastq.gz
2.46 GB
-
Dobos_msmegribcko_2_S20_L002_R1_001.fastq.gz
2.62 GB
-
Dobos_msmegribcko_2_S20_L002_R2_001.fastq.gz
2.62 GB
-
Dobos_msmegribgko_6_S24_L002_R1_001.fastq.gz
1.80 GB
-
Dobos_msmegribgko_6_S24_L002_R2_001.fastq.gz
1.79 GB
-
Dobos_msmegribhcomp_3_S21_L002_R1_001.fastq.gz
2.84 GB
-
Dobos_msmegribhcomp_3_S21_L002_R2_001.fastq.gz
2.83 GB
-
Dobos_msmegribhko_4_S22_L002_R1_001.fastq.gz
2.15 GB
-
Dobos_msmegribhko_4_S22_L002_R2_001.fastq.gz
2.14 GB
-
Dobos_msmegwt_9_S27_L002_R1_001.fastq.gz
1.94 GB
-
Dobos_msmegwt_9_S27_L002_R2_001.fastq.gz
1.92 GB
-
Dobos_mtbriba2comp_16_S34_L002_R1_001.fastq.gz
2.35 GB
-
Dobos_mtbriba2comp_16_S34_L002_R2_001.fastq.gz
2.36 GB
-
Dobos_mtbriba2ko_17_S35_L002_R1_001.fastq.gz
2.09 GB
-
Dobos_mtbriba2ko_17_S35_L002_R2_001.fastq.gz
2.09 GB
-
Dobos_mtbribccomp10_S28_L002_R1_001.fastq.gz
2.58 GB
-
Dobos_mtbribccomp10_S28_L002_R2_001.fastq.gz
2.95 GB
-
Dobos_mtbribcko_11_S29_L002_R1_001.fastq.gz
1.79 GB
-
Dobos_mtbribcko_11_S29_L002_R2_001.fastq.gz
1.78 GB
-
README.md
3.69 KB
Abstract
This dataset contains raw whole-genome sequencing data (FASTQ files) for wild-type and riboflavin biosynthetic gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To confirm successful gene deletions and rule out off-target effects, whole-genome sequencing was performed for each mutant and its corresponding wild-type strain. This dataset supports the verification of targeted genetic modifications and enables comparative genomic analyses related to MR1 antigen production in mycobacteria.
Dataset DOI: 10.5061/dryad.4tmpg4fnv
Description of the data and file structure
Whole Genome Sequence of wild type strain and riboflavin biosynthetic pathway mutants of Mycobacterium tuberculosis (Mtb) and Mycolicibacterium smegmatis (Msm). Also, this dataset has part 2 which is available at https://doi.org/10.5061/dryad.vq83bk454
Naming convention for files:
- Lab name 2. Strain Name 3. Sample Number 4. Batch Number 5. Read (Forward/Reverse) 6. Replicate
e.g. For file name "Dobos_msmegriba2comp_7_S25_L002_R2_001": Lab name: Dobos; Strain Name: msmegriba2comp_7; Sample Number: S25; Batch Number: L002; Read: R2 (Reverse Read); Replicate: 001
Files and variables
File: Dobos_mtbribcko_11_S29_L002_R2_001.fastq
Description: Reverse Read for Mtb ΔribC
File: Dobos_msmegriba2comp_7_S25_L002_R2_001.fastq.gz
Description: Forward Read for Msm ΔribG::ribG
File: D0bos_msmegribgcomp_5_S23_L002_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribG::ribG
File: Dobos_msmegribccomp_1_S19_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribC::ribC
File: Dobos_msmegribgko_6_S24_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribG
File: Dobos_msmegribgko_6_S24_L002_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribG
File: Dobos_msmegwt_9_S27_L002_R2_001.fastq.gz
Description: Reverse Read for Msm Wild Type
File: Dobos_msmegwt_9_S27_L002_R1_001.fastq.gz
Description: Forward Read for Msm Wild Type
File: Dobos_mtbriba2ko_17_S35_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribA2
File: Dobos_mtbriba2ko_17_S35_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribA2
File: Dobos_msmegribhko_4_S22_L002_R2_001.fastq.gz
**Description: **Reverse Read for Msm ΔribH
File: Dobos_msmegribhko_4_S22_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribH
File: Dobos_msmegriba2comp_7_S25_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribA2::ribA2
File: Dobos_msmegribccomp_1_S19_L002_R2_001.fastq.gz
Description: Forward Read for Msm ΔribC::ribC
File: Dobos_mtbriba2comp_16_S34_L002_R1_001.fastq.gz
Description: Reverse Read for Mtb ΔribA2::ribA2
File: Dobos_mtbriba2comp_16_S34_L002_R2_001.fastq.gz
Description: Reverse Read for Mtb ΔribA2::ribA2
File: Dobos_msmegribcko_2_S20_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribC
File: Dobos_msmegribcko_2_S20_L002_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribC
File: Dobos_mtbribccomp10_S28_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribC::ribC
File: Dobos_msmegribhcomp_3_S21_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribA2::ribA2
File: Dobos_msmegribhcomp_3_S21_L002_R2_001.fastq.gz
Description: Reverse Read for Msm ΔribH::ribH
File: D0bos_msmegribgcomp_5_S23_L002_R1_001.fastq.gz
Description: Forward Read for Msm ΔribG::ribG
File: Dobos_mtbribccomp10_S28_L002_R2_001.fastq.gz
Description: Forward Read for Mtb ΔribC::ribC
File: Dobos_mtbribcko_11_S29_L002_R1_001.fastq.gz
Description: Forward Read for Mtb ΔribC