Dataset DOI: 10.5061/dryad.5mkkwh7h4

Description of the data and file structure

In brief, we have examined different genetically encoded calcium indicators constructs in-vitro in HEK293t cells and rat neurons, and in-vivo in mice and larval Zebrafish.

All of the construct names (GCaMP6s2, GCaMP6s3.0 and so on, marked in bold) are genetically encoded calcium indicators for which the exact structure and sequence can be found in the published article.

The files are named as references to the figure/subset of a figure they are related to.
The .pzfx files are GraphPad Prism files which contain the data used to graph. For all files and specifically those which reference multiple figures/subset of figures are also named and referenced by the associated figure/subset of figure inside the Prism files. The names of each construct used are also given in each file. Some aesthetical changes from the actual published article figures are present.

The following are the result of analyzing fluorescence recordings of different calcium indicator constructs and represent the post analysis maximal ΔF/F (the maximal change in fluorescence recorded for each cell that expressed the construct):

• Fig1b_j_Fig2c_g.pzfx :
  Fig1b - The ΔF/F of GCaMP6s2, GCaMP6s3.0, GCaMP6s3.1 and GCaMP6s3.2  expressed in-vitro from rat neurons.

  Fig1j - The ΔF/F of M13 cpGFP and M13 cpGFP + CaM expressed in-vitro from rat neurons in soma and dendrites.

  Fig2c - The ΔF/F before and after ionomycin of activated PA-sf-S2 and non-activated PA-sf-S2 expressed in-vitro from HEK293t cells.

  Fig2g - The ΔF/F before and after ionomycin of sfGCaMP6s₂, osfGCaMP6s₂ and GCaMP6s2 expressed in-vitro from HEK293t cells.

• Fig1g.pzfx - The ΔF/F of M13-cpGFP and Calmodulin expressed in soma and in dendrites in-vitro from rat neurons.

• Fig3-b_c.pzfx - The ΔF/F of GCaMP6s, Split-GCaMP6s3.0 and Split-sf-S2 expressed in soma and in dendrites in-vitro from rat neurons.

• fig4d.pzfx - The ΔF/F before and after Calcium addition of sfGFP1–6 and sfGFP7–11, and sfGFP1–10 and sfGFP11 expressed in-vitro from HEK293t cells.

• Fig5f-left.pzfx - The ΔF/F of Split-sf-MEGIC₂ in soma and in dendrites recorded in-vitro from rat neurons, including all events and then categorized by number of action potentials during each calcium transient.

• Fig5h.pzfx - The ΔF/F of Split-sf-MEGIC₂ recorded in-vivo in larval Zebrafish.

The following data also includes a decay time Tau_1/2 analysis which represent how long it takes the fluorescence spike to decay (related to Figure 1).

• Fig1g.pzfx - The decay time of M13-cpGFP and Calmodulin expressed in-vitro from rat neurons.

The following data represents the basal fluorescence intensity recorded for each construct (related to Figure 4).

• Fig4b.pzfx - The basal fluorescence intensity of sfGFP1–6 and sfGFP7–11, and sfGFP1–10 and sfGFP11 expressed in-vitro from HEK293t cells.

The following data represents Cross-Correlation calculations between neuronal spiking and each construct activation (related to Figure 5).

• Fig5f-right.pzfx - Cross-Correlation calculations between either Split-sf-MEGIC2 or GCaMP6s wt activity with neuronal action potentials in-vitro from rat neurons. 

The following are the raw 2-photon recordings of split-sf-MEGIC₂ in mice brains in the primary motor cortex (related to Figure 7):

• Fig7_FOV1_trial5.avi
• Fig7_FOV2_trial2.avi
• Fig7_FOV2_trial3.avi
• Fig7_FOV2_trial4.avi
• Fig7_FOV2_trial5.avi
• Fig7_FOV2_trial6.avi
• Fig7_FOV2_trial9.avi
• Fig7_FOV4_trial1.avi
• Fig7_FOV4_trial10.avi
• Fig7_FOV4_trial11.avi
• Fig7_FOV4_trial2.avi
• Fig7_FOV4_trial3.avi
• Fig7_FOV4_trial4.avi
• Fig7_FOV4_trial5.avi
• Fig7_FOV4_trial6.avi
• Fig7_FOV4_trial7.avi
• Fig7_FOV4_trial8.avi
• Fig7_FOV4_trial9.avi

Code/software

GraphPad Prism 8.

Access information

Other publicly accessible locations of the data:

• None.

Data was derived from the following sources:

• Data was not derived from other sources.