Historical effects during experimental evolution of multicellularity in Saccharomyces cerevisiae
Data files
Oct 28, 2025 version files 18.52 MB
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Cluster-Phenotype-Angle.csv
4.27 KB
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Cluster-Phenotype-C10W8.jpg
46.34 KB
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Cluster-Phenotype-M2A3-2.jpg
125.34 KB
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Cluster-Phenotype-M2B2-3.jpg
143.87 KB
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Cluster-Phenotype-M5A1-2.jpg
158.83 KB
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Cluster-Phenotype-M5A1-2W_Angle.jpg
185.84 KB
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Cluster-Phenotype-M5A2-4.jpg
445.01 KB
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Cluster-Phenotype-M5A3-2.jpg
198.77 KB
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Cluster-Phenotype-Ratio_-.csv
6.93 KB
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Experimental_evolution-Sizes-Day30.csv
13.08 KB
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Experimental-Evolution-Sizes-Ancestors.csv
13.97 KB
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Experimental-Evolution-Tempo-data.csv
539 B
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README.md
5.63 KB
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Tetrad-Dissection-U2A-pic1.JPG
2.46 MB
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Tetrad-Dissection-U2B-picture1.JPG
2.44 MB
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Tetrad-Dissection-U2C-1.JPG
2.43 MB
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Tetrad-Dissection-U2C-2-picture1.JPG
2.38 MB
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Tetrad-Dissection-U3A-picture3.JPG
2.38 MB
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Tetrad-Dissection-U5A.JPG
2.57 MB
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Tetrad-Dissection-y55_self.JPG
2.51 MB
Abstract
Timing and phenotype of the recovery of multicellularity in populations of Saccharomyces cerevisiae. Data includes results on the recovery of multicellularity from selfing (bulk and tetrad) and experimental evolution for increased settling. Data pictures of tetrad dissections, size distributions of individuals, the timing at which multicellularity was first observed, and images of multicellular individuals.
Description of the data and file structure
The propensity for multicellular phenotypes arising from ten different diploid Saccharomyces cerevisiae unicellular genotypes was studied. The appearance of multicellularity was investigated by selfing (tetrad dissection and bulk) and experimental evolution using settling selection. For a subset of multicellular individuals, we characterized the multicellular phenotypes.
Files and variables
File: Tetrad-Dissection-U2A-pic1.JPG
Description: Dissection of Sporulated U2A genotype, one image.
File: Tetrad-Dissection-U2B-picture1.JPG
Description: Dissection of Sporulated U2B genotype, one image.
File: Tetrad-Dissection-U2C-1.JPG
Description: Dissection of Sporulated U2C genotype, a dissection image.
File: Tetrad-Dissection-U2C-2-picture1.JPG
Description: Dissection of Sporulated U2C genotype, a second dissection, one image.
File: Tetrad-Dissection-U3A-picture3.JPG
Description: Dissection of Sporulated U3A genotype, one image.
File: Tetrad-Dissection-U5A.JPG
Description: Dissection of Sporulated U5A genotype, one image.
File: Tetrad-Dissection-y55_self.JPG
Description: Dissection of Sporulated Y55 genotype, one image.
File: Cluster-Phenotype-C10W8.jpg
Description: Analyzed structure of experimentally evolved genotype C10W8.
File: Cluster-Phenotype-M2A3-2.jpg
Description: Analyzed structure of experimentally evolved genotype M2A3.
File: Cluster-Phenotype-M2B2-3.jpg
Description: Analyzed structure of experimentally evolved genotype M2B2.
File: Cluster-Phenotype-M5A1-2.jpg
Description: Analyzed structure of experimentally evolved genotype M5A1.
File: Cluster-Phenotype-M5A1-2W_Angle.jpg
Description: Analyzed structure of experimentally evolved genotype M5A1, focussing on the Angle.
File: Cluster-Phenotype-M5A2-4.jpg
Description: Analyzed structure of experimentally evolved genotype M5A2.
File: Cluster-Phenotype-M5A3-2.jpg
Description: Analyzed structure of experimentally evolved genotype M5A3.
File: Cluster-Phenotype-Angle.csv
Description: Angle data for the different multicellular phenotypes
Variables
- Strain: Multicellular strain
- Angle: Budding angle (degrees).
- Rep: Replicate
- Phenotype: Normal vs. Pseudohyphae
File: Cluster-Phenotype-Ratio_-.csv
Description:
Variables
- Strain: Multicellular strain
- Ratio: Length/width (no units)
- Rep: Replicate
- Phenotype: Normal vs. Pseudohyphae
File: Experimental-Evolution-Tempo-data.csv
Description: Number of days (transfers) required for multicellularity to evolve
Variables
- Day: Day
- Lineage: Population under selection
- Anc: Immediate Ancestor of replicate populations (lineages)
- Ancient: Previous Ancestor.
File: Experimental-Evolution-Sizes-Ancestors.csv
Description: Size distributions of genotypes used in settling selection, prior to any selection.
Variables
- volume_um3: Volume in cubic microns
- diameter_um: Diameter in microns.
- blank: Control size run.
- U4A: Number of individuals in each size class for U4A.
- U1A: Number of individuals in each size class for U1A.
- U3A: Number of individuals in each size class for U3A.
- U2A: Number of individuals in each size class for U2A.
- U2B: Number of individuals in each size class for U2B.
- U5A: Number of individuals in each size class for U5A.
- U2C: Number of individuals in each size class for U2C.
- U5B: Number of individuals in each size class for U5B.
- U4C: Number of individuals in each size class for U4C.
- Y55: Number of individuals in each size class for Y55.
File: Experimental_evolution-Sizes-Day30.csv
Description: Size distributions of populations after 30 days of selection.
Variables
- diameter_um: Volume in cubic microns
- Y55: Number of individuals in each size class for Y55.
- U4A: Number of individuals in each size class for U4A.
- U1A: Number of individuals in each size class for U1A.
- U3A: Number of individuals in each size class for U3A.
- U2A: Number of individuals in each size class for U2A.
- U2B: Number of individuals in each size class for U2B.
- U5A: Number of individuals in each size class for U5A.
- U2C: Number of individuals in each size class for U2C.
- U4B: Number of individuals in each size class for U4B.
- U4C: Number of individuals in each size class for U4C.
File: Bulkselfing-Sizes.csv
Description: Size distributions of populations derived by bulk selfing of genotypes.
Variables
- diameter_um: Volume in cubic microns
- U4A: Number of individuals in each size class for U4A.
- U1A: Number of individuals in each size class for U1A.
- U3A: Number of individuals in each size class for U3A.
- U2A: Number of individuals in each size class for U2A.
- U2B: Number of individuals in each size class for U2B.
- U5A: Number of individuals in each size class for U5A.
- U2C: Number of individuals in each size class for U2C.
- U4B: Number of individuals in each size class for U4B.
- U4C: Number of individuals in each size class for U4C.
- Y55: Number of individuals in each size class for Y55.
Code/software
Budding angle and cell size were determined by ImageJ2 (Rueden et al. 2017). Size distributions were initially visualized and analyzed using RStudio version 1.4.1717, and later redrawn using JMP and Excel.