Data from: Droplet-based single-cell pairing for high-throughput interaction mapping of antigen-receptor combinations
Data files
Mar 02, 2026 version files 1.31 MB
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Fig._3A.opju
848.69 KB
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Fig._3B.opju
102.90 KB
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Fig._3E.opju
54.50 KB
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Fig._S3A.opju
101.36 KB
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Fig._S3B.opju
103.76 KB
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Fig._S4.opju
93.31 KB
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README.md
2.79 KB
Abstract
Mapping the interaction potential of different variant combinations of viral antigens and human cell receptors and understanding how viral antigen mutations interact with human genetic polymorphisms are critical for predicting infection susceptibility and informing precision public health strategies. Here, we develop a droplet-based single-cell pairing and library-on-library interaction screening (SPLIS) system for high-throughput profiling of the syncytium-formation landscapes of various spike-angiotensin-converting enzyme 2 (ACE2) variant combinations. This system uses combined droplet sorting and merging to deterministically encapsulate one antigen-presenting sender cell and one receptor-expressing receiver cell into each drop, followed by selection and sequencing of the fused DNA readouts to characterize the syncytium-formation potential of each combination. We applied SPLIS to characterize both fusion-enhancing and -inhibiting variant pairs, comprehensively profiling how ACE2 single-nucleotide polymorphisms (SNPs) modulate susceptibility to emerging SARS-CoV-2 spike mutations. Our system emerges as a powerful tool to interrogate the interactions between two libraries of variants, offering valuable insights into host susceptibility patterns and viral infectivity trends.
Dataset DOI: 10.5061/dryad.6djh9w1fw
Description of the data and file structure
We have submitted our raw data for Critical evaluation of droplet manipulations with (Fig._3A.opju, Fig._3B.opju and Fig._3E.opju). We have also added raw data for Validation of droplet synchronization on nonuniformly sized droplets (Fig._S3A.opju and Fig._S3B.opju), as well as raw data for Encounter frequency at different sizes of droplets (Fig._S4.opju).
Files and variables
File: Fig._3A.opju
Description: The file contains 6 tables for Fig. 3A.
- The red square contains tested and fitted plots showing the droplet diameter (μm) and generation frequency when the water flow rate is at 100 μL/h.
- The green square contains tested and fitted plots showing the droplet diameter (μm) and generation frequency when the water flow rate is at 200 μL/h.
- The blue square contains tested and fitted plots showing the droplet diameter (μm) and generation frequency when the water flow rate is at 300 μL/h.
- The red circle contains tested and fitted plots showing the generation frequency (Hz) when the water flow rate is at 100 μL/h.
- The green circle contains tested and fitted plots showing the generation frequency (Hz) when the water flow rate is at 200 μL/h.
- The blue circle contains tested and fitted plots showing the generation frequency (Hz) when the water flow rate is at 300 μL/h.
File: Fig._3B.opju
Description: File contains 1 table showing histograms depicting the fraction of droplets containing different numbers of cells for Fig. 3B. It has one column showing the fraction of droplets containing different numbers of cells (%).
File: Fig._3E.opju
Description: The file contains 1 table showing the variation in the percentage of fused cells as time changes for Fig. 3E. It contains one column showing the fusion rate (%).
File: Fig._S3A.opju
Description: File contains 1 table showing the efficiency of droplet synchronization on nonuniformly sized small droplets for Fig. S3A. It contains one column showing the percentage of one-to-one pairing (%).
File: Fig._S3B.opju
Description: File contains 1 table showing the efficiency of droplet synchronization on nonuniformly sized large droplets for Fig. S3A. It contains one column showing the percentage of one-to-one pairing (%).
File: Fig._S4.opju
Description: File contains 1 table showingEncounter frequency at different sizes of droplets for Fig. S3A. It contains one column showing the cell encounter frequency (times/ minute).