https://doi.org/10.5061/dryad.6m905qg61

This dataset was used to conduct a meta-analysis on the effects of temperature on the nitrogen excretion rates of freshwater fish.

Description of the data

MA_dataset.xlsx

This file is divided in two sheets. The first is titled "Search" and the second is titled "Dataset".

The "Search" sheet contains all scientific papers that appeared in our search.

• Each paper received an identification number under the column "Study ID".
• The columns "Author", "Year", "Journal" and "Title" informs the respective authors, year of publication, journal where it was published and the title of the paper.
• The column "Source" informs the type of publication and the column "Fits search criteria" informs if this reference fitted our search criteria. The column "Database" informs the database in which the study appeared.

The "Dataset" sheet contains the information that was extracted from each paper included in the meta-analysis.

• The columns "Study ID", "Author" and "Year" inform the study identification number, its author's and year of publication. The column "Species" indicate the scientific name of the studied species of fish.
• The column "Source of data in the paper" informs where the information was extracted from each study (a table or figure, abbreviated as Fig, followed by its respective number). The column "Effect size" informs the type of effect size being extracted in the study, which is "Mean comparison".
• The columns "Control temperature" and "Treatment temperature" informs the temperatures used in the experiment in Celsius degrees. The lowest temperature used in the experiment was considered as the "Control temperature" and the highest as the "Treatment temperature".
• The columns "Control mean excretion", "Control error" and "N control" indicates the mean nitrogen excretion rates for the fish under the Control temperature, its respective error measure (as standard deviation) and sample size (which is the number of individuals tested). The same is informed for the fish under the Treatement temperature under the columns "Treatment mean excretion", "Treatment error" and "N treatment".
• The column "Error type" informs that the measure of error used is Standard Deviation, abbreviated as SD.
• The column "Excreted element" informs how nitrogen was measured: NH3 means ammonia, NH4 means ammonium, TN (NH4 + NH3) means Total Nitrogen (ammonium + ammonia), and ureaN means urea.
• The column "Excretion rate unit" indicates the unit used for the excretion rates of nitrogen, which is as umol of nitrogen per gram of fish per hour (umol/g/h).
• The column "Feeding status" informs if the fish used in the experiment was starved of fed before excretion measurements.
• The column "Starvation period (days)" informs for how many days the fish were starved before the nitrogen excretion measurements.
• The column "Acclimation period (days)" indicates for how many days the fish stayed under the experimental temperatures before the nitrogen excretion measurements.
• The column "Weight (g)" details the fish wet weight in grams.
• The column "Delta temperature" represents the difference between the Treatment and Control temperatures. The column "Climate" indicates the species climate occurrence.
• The column "OBS" is for relevant information regarding the study.

dataset.csv

This file was used to conduct the meta-analysis in R software. The file is semicolon-delimited with a comma decimal.

• The columns "Author" and "Year" informs the respective authors and year of publication of the studies. "Study ID" informs the identification number of the study, and "Case ID" informs the identification number for each case-study within each study.
• The columns "sp"  and "order" informs the species name and taxonomic order of the fish, respectively. The species names were abreviated and mean, in this order: "cyp" for Cyprinus carpio, "dor" for Dorosoma cepedianum, "sin" for Siniperca chuatsi, "cte" for Ctenopharyngodon idellus, "ore" for Oreochromis nilotica, "sal" for Salmo salar, "lep" for Lepomis macrochirus, "sal" for Salvelinus alpinus, "abr" for Abramis brama L., "cha" for Channa punctatus, "sti" for Stizostedion vitreum, "til" for Tilapia mossambica, "pho" for Phoxinus phoxinus, "onc" for Oncorhynchus mykiss, "bid" for Bidyanus bidyanus, "eso" for Esox lucius L., "pal" for Palmetto bass, "umb" for Umbra limi, "gam" for Gambusia affinis, "dan" for Danio rerio, "car" for Carassius auratus, "san" for Sander lucioperca, "c.cognatus" for Cottus cognatus, "lep" for Leptobarbus hoevenii, and "ore" for Oreochromis alcalicus grahami.
• The column "starved" informs whether the fish was starved before excretion trials, with "yes" for those who were starved and no for those who were not.
• The column "Acclimatization.time" informs the period of time (days) in which the fish was acclimated to the new temperature, with "n/a" meaning "not applicable" for studies conducted in the field or studies that did not employ any acclimatization period to the new temperature, and "not informed" for studies that did not inform the acclimatization period.
• The column "weight" informs the fishes weight in grams. 
• The column "Source" informs where the information was extracted from each study (a table or figure, abreviated as Fig, followed by its respective number).
• The columns "temp_control" and "treat_temp" inform the temperature in the control and in the experimental treatment both in degrees Celsius, respectively.
• The column "temp_diff" refers to the delta temperature, which is the difference between the treatment and control temperatures, in Celsius. "Ne" is the sample size, "Me" is the mean excretion rate (µmol N/g/h), and "Se" is the standard deviation for the experimental treatment, and "Nc", "Mc", "Sc" is the same for the control treatment.
• The column "starvation period" informs the period of starvation that the fish was subjected to in days. The column "climate" informs the fish's climatic region.

script_LO.R

This file is the R software script used for conducting the statistical analysis of the manuscript.