Dataset DOI: 10.5061/dryad.70rxwdcc1

Description of the data and file structure

The contents of this dataset were used in the associated preprint "Ballistic Microscopy (BaM)." It includes multiple data, including confocal fluorescence microscopy, transmission electron microscopy (TEM), cryo-electron microscopy (cryo-EM), high-speed imaging, mass spectrometry data from CLIP-170 condensates, and protein gel images.

This dataset contains several types of data used in the manuscript. File names are organized and labeled according to main figures, supplementary figures, videos, and supplementary tables.

.xlsx files can be opened using Microsoft Excel; .mrc and .tif files can be opened using ImageJ/Fiji; .cys files can be opened using Cytoscape; and .stl files can be opened using PrusaSlicer.

01 Table S2. List of proteins in CLIP170 condensates.pdf

• 01_Table_S2._List_of_proteins_in_CLIP170_condensates.pdf
  • This file contains the list of proteins identified in CLIP-170 condensates, derived from pooled BaM mass spectrometry experiments. Proteins are listed with associated annotations used in the manuscript.

02 Table S3 Materials reagents and antibodies.xlsx

• Table_S3_Materials__reagents_and_antibodies.xlsx
  • This Excel file provides a comprehensive list of materials, reagents, and antibodies used in this study, including sources and identifiers, following standard reporting guidelines.
• Variables (Excel/ Spreadsheet Data):
  • Resource or Reagent - Name of the material, reagent, or antibody.
  • Source - Supplier or manufacturer.
  • Identifier - Catalog number, or other unique identifier.
  • All variables are categorical text fields and do not have physical units.

Fig. 4G and SV3a Cryo-EM tilt series.mrc

• Fig._4G_and_SV3a_Cryo-EM_tilt_series.mrc

  This file contains the raw cryo-electron microscopy tilt series used for structural analysis and visualization associated with Figure 4G and Supplementary Video 3a.

Fig. 5F GFP-CLIP170 BaM mass spectrometry.cys

• Fig._5F_GFP-CLIP170_BaM_mass_spectrometry.cys
  • Cytoscape network file representing the protein interaction network identified from BaM mass spectrometry of GFP-CLIP-170 condensates.

Fig. 5G GFP control BaM mass spectrometry.cys

• Fig._5G_GFP_control_BaM_mass_spectrometry.cys
  • Cytoscape network file representing the protein interaction network obtained from BaM mass spectrometry of GFP control samples.

Fig. 5J Fig. S8H SV4 CLIP 170 Keratin 14 Z stack panel A.tif

Fig. S8I SV4 CLIP 170 Keratin 14 Z stack panel B.tif

• Fig._5J_Fig.S8H_SV4_CLIP_170_Keratin_14_Z_stack_panel_A.tif
• Fig.S8I_SV4_CLIP_170_Keratin_14_Z_stack_panel_B.tif
  • Fluorescence microscopy Z-stack images showing CLIP170 and Keratin-14 localization and organization, corresponding to main and supplementary figures and videos.

Fig. 5L Fig. S8J SV5 Tau 3R Keratin 18 Z stack.tif

• Fig._5L_Fig.S8J_SV5_Tau_3R_Keratin_18_Z_stack.tif
  • Fluorescence microscopy Z-stack image illustrating Tau 3R and Keratin-18 organization in condensates and aggregates.

Fig. S4D TEM panel a.tif

Fig. S4D TEM panel b.tif

Transmission electron microscopy images associated with Supplementary Figure S4D.

Fig. S4E TEM panel a-h.tif

• Fig._S4D_TEM_panel_a.tif

• Fig._S4D_TEM_panel_b.tif

• Fig._S4E_TEM_panel_a.tif

• Fig._S4E_TEM_panel_b.tif

• Fig._S4E_TEM_panel_c.tif

• Fig._S4E_TEM_panel_d.tif

• Fig._S4E_TEM_panel_e.tif

• Fig._S4E_TEM_panel_f.tif

• Fig._S4E_TEM_panel_g.tif

• Fig._S4E_TEM_panel_h.tif

  Transmission electron microscopy panels corresponding to Supplementary Figure S4E, showing ultrastructural features observed in BaM-related experiments.

Fig. S6C protein gel.tif

• Fig._S6C_protein_gel.tif
  • Protein gel image used to validate protein expression and purification steps described in the manuscript.

Fig. S5 Cryo-EM panel a-c.tif

Fig. S5 Cryo-EM BaM panel d-l.tif

• Fig.S5_Cryo-EM_BaM_panel_d.tif

• Fig.S5_Cryo-EM_BaM_panel_e.tif

• Fig.S5_Cryo-EM_BaM_panel_f.tif

• Fig.S5_Cryo-EM_BaM_panel_g.tif

• Fig.S5_Cryo-EM_BaM_panel_h.tif

• Fig.S5_Cryo-EM_BaM_panel_i.tif

• Fig.S5_Cryo-EM_BaM_panel_j.tif

• Fig.S5_Cryo-EM_BaM_panel_k.tif

• Fig.S5_Cryo-EM_BaM_panel_l.tif

• Fig.S5_Cryo-EM_panel_a.tif

• Fig.S5_Cryo-EM_panel_b.tif

• Fig.S5_Cryo-EM_panel_c.tif

  Cryo-electron microscopy images associated with Supplementary Figure S5, including conventional cryo-EM and BaM-derived samples.

File to 3D print an EM grid holder that fits in a PCR tube.zip

• File_to_3D_print_an_EM_grid_holder_that_fits_in_a_PCR_tube.zip
  • This archive contains STL files required to 3D-print a custom EM grid holder designed to fit inside a PCR tube, as described in the methods.

Code/Software

No new code was generated in this study.