Dataset DOI: 10.5061/dryad.76hdr7t8r

Description of the data and file structure

This dataset contains morphometry, echocardiography, electrophysiology (action potentials and ionic currents), fluorescent Ca imaging, and RNA sequencing data in separate Excel sheets with individual cell data and group statistics. Files are compressed to keep the folder structure and to ease navigation between the uploaded documents. For a full description of the materials and methods, including experimental procedures and instrumentation, please refer to the article on the American Journal of Physiology - Heart and Circulatory Physiology website.

File: Action_potential.zip

Description: This compressed file contains two Excel files with action potential (AP) data in 3 groups of experimental animals, including (1) control sham, (2) leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported separately in each group.

"Fig3_data_AP-parameters.xlsx" provides ventricular cardiomyocyte AP data at 1 Hz steady-state pacing frequency. Sheet "Panel A" provides typical AP waveforms data (time in milliseconds and membrane voltage in millivolts). Sheet "Panel B" provides AP repolarization parameters, including AP duration at 20, 50, and 90% of repolarization (APD20, APD50, and APD90, respectively) in milliseconds. Sheet "Panel C" provides additional AP parameters, including resting membrane potential (RMP, in millivolts), maximal rate of AP upstroke velocity (dV/dtmax, in volts per second), and maximal rate of AP phase 3 repolarization (-dV/dtmax, in volts per second).

"Fig4_data_Frequency-STV-Alternans.xlsx" provides ventricular cardiomyocyte AP data at various pacing rates from 1 Hz to 10 Hz. Sheet "Panel A" provides data about the frequency dependence of AP duration at 90% of repolarization (APD90, in milliseconds). Sheet "Panel B" provides typical AP waveforms data at a 10 Hz pacing rate (time in milliseconds and membrane voltage in millivolts). Sheet "Panel C" provides fifty consecutive APD90 data at a 1 Hz pacing rate (time in seconds, APD90 in milliseconds). Sheet "Panel D" provides the short-term variability of APD90 at 1 Hz pacing (STV, in milliseconds), the magnitude of APD90 alternans (difference between long and short beats) at 10 Hz pacing (in milliseconds).

File: Ca_imaging.zip

Description: This compressed file contains two Excel files with fluorescent intracellular Ca2+ imaging (using Fluo-4) data in 3 groups of experimental animals, including (1) control sham, (2) leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported separately in each group.

"Fig10_data_CaT.xlsx" provides ventricular cardiomyocyte intracellular Ca2+ transient (CaT) data. Sheet "Panel B" provides typical caffeine-induced Ca2+ transient (Caff T) data (time in milliseconds and fluorescence expressed as background corrected F/F0, where F0 is the non-paced baseline fluorescence). Sheet "Panel C" provides CaT parameters data at 1 Hz steady-state pacing, including CaT amplitude (difference between baseline and peak fluorescence, normalized to non-paced F0), CaT tau decay (in milliseconds), and sarcoplasmic reticulum (SR) Ca2+ load assessed by rapid caffeine application (difference between baseline and peak fluorescence, normalized to non-paced F0). Sheet "Panel D" provides CaT parameters at 2 Hz steady-state pacing, including CaT amplitude (difference between baseline and peak fluorescence, normalized to non-paced F0), CaT tau decay (in milliseconds), and Caff T decay slope (change in fluorescence per second).

"Fig11_data_Ca-sparks-and-waves.xlsx" provides diastolic Ca2+ spark data in ventricular cardiomyocytes. Sheet "Panel B" provides Ca2+ spark frequency (number of Ca2+ sparks per 100 micrometers per second) and Ca2+ wave frequency (number of waves per minute).

File: Echocardiography.zip

Description: This compressed file contains one Excel file with transthoracic echocardiography data in 3 groups of experimental animals, including (1) control sham, (2) leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported separately in each group.

"Fig2_data_Echocardiography.xlsx" provides key echocardiographic parameters to characterize systolic and diastolic heart function. Sheet "Panel B" provides systolic heart function parameters: ejection fraction (EF, %), left ventricular (LV) mass (in milligrams), and left ventricular remodeling index (LVRI, milligram/millimeter) data. Sheet "Panel C" provides diastolic heart function parameters: early to late filling velocity ratio (ratio between mitral E wave and A wave, E/A), ratio between mitral E wave and e’ wave (E/e'), and left atrial (LA) area (in square millimeters) data.

File: Ionic_currents.zip

Description: This compressed file contains five Excel files with cellular electrophysiology data in 3 groups of experimental animals, including (1) control sham, (2) leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported separately in each group.

"Fig5_data_Capacitance.xlsx" provides cell membrane capacitance (in picofarads)

"Fig6_data_IK1.xlsx" provides data on the inward rectifier potassium current (IK1). Sheet "Panel A" provides typical IK1 traces (time in milliseconds and IK1 current in picoampere per picofarad). Sheet "Panel B" provides the net magnitude of IK1 at -140 millivolts and at -40 millivolts (in picoamperes). Sheet "Panel C" provides the density of IK1 (IK1 magnitude normalized to cell capacitance) at -140 millivolts and at -40 millivolts (in picoampere per picofarad).

"Fig7_data_IKv.xlsx" provides data on the voltage-gated potassium currents (IKv). Sheet "Panel A" provides typical IKv traces (time in milliseconds and IKv current in picoampere per picofarad). Sheet "Panel B" provides the net magnitude of the transient outward K+ current (Ito), the slowly inactivating potassium current (IK, slow), and the sustained potassium current (Isus) at 60 millivolts (in picoamperes). These potassium currents were separated by biexponential fitting to I_Kv traces. Sheet "Panel C" provides the density of Ito, IK, slow, and Isus (magnitude normalized to cell capacitance) at 60 millivolts (in picoampere per picofarad).

"Fig8_data_ICa,L.xlsx" provides data on the L-type calcium current (ICa,L). Sheet "Panel A" provides typical ICa,L traces (time in milliseconds and ICa,L current in picoampere per picofarad). Sheet "Panel B" provides the voltage-dependence of ICa,L (current-voltage relationship, I-V). Voltage steps are expressed in millivolts, and currents are expressed in picoampere per picofarad. Sheet "Panel C" provides the net magnitude (in picoamperes) and the density (magnitude normalized to cell capacitance, in picoampere per picofarad) of ICa,L.

"Fig9_data_INa,L.xslx" provides data on the late sodium current (INa,L). Sheet "Panel A" provides typical INa,L traces (time in milliseconds and INa,L current in picoampere per picofarad). Sheet "Panel B" provides the net magnitude (in picoamperes) and the density (magnitude normalized to cell capacitance, in picoampere per picofarad) of INa,L.

File: Morphometry.zip

Description: This compressed file contains one Excel file with morphometric data in 3 groups of experimental animals, including (1) control sham, (2) leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo), and (3) high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME). Male and female data are reported separately in each group.

"Fig1_Data_Morphometry.xlsx" provides key morphometric data. Sheet "Panel B" provides body weight data (in grams). Sheet "Panel C" provides blood glucose data (in milligrams/deciliter). Sheet "Panel D" provides heart weight to tibial length ratio (HW/Tl, in gram/centimeter). Sheet "Panel E" provides wet to wet-to-dry lung weight ratio. Sheet "Panel F" provides liver weight (in grams).

File: RNA-sequencing.zip

Description: This compressed file contains 5 Excel files with RNA-sequencing data.

"Data Set S1_dbdb+Aldo_vs_Vehicle_Control_DEGenes.xslx" provides differentially expressed genes (DEGs) in leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo) murine hearts versus wild-type sham controls.

"Data Set S2_HFD+L-NAME_vs_Chow_Control_DEGenes.xlsx" provides differentially expressed genes (DEGs) in high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME) murine hearts versus normal chow controls.

"Data Set S3_Model Comparison Genes Labelled.xlsx" provides differentially expressed genes (DEGs) comparison between two HFpEF murine models: leptin receptor-deficient db/db mice with chronic aldosterone infusion (DB), and high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD).

"Data Set S4_dbdb+Aldo_female_vs_male_DEGenes.xlsx" provides differentially expressed genes (DEGs) in female versus male hearts in the leptin receptor-deficient db/db mice with chronic aldosterone infusion (db/db+Aldo) model.

"Data Set S5_HFD+L-NAME_female_vs_male_DEGenes.xlsx" provides differentially expressed genes (DEGs) in female versus male hearts in the high-fat diet combined with nitric oxide synthase inhibitor L-NAME treatment (HFD+L-NAME) model.

Code/software

Microsoft Excel can be used to view the data files.