Dataset DOI: 10.5061/dryad.83bk3jb6q

Description of the data and file structure

File name: Data.xlsx

Below you find a list of datasets with a summary of variable names and a brief description of the data. Missing values are indicated with “NA”:

Across all sheets:

• E_ID: plant individual identity

• E_SEX: plant sex (F=female, M=male)

• E_TMT: aphid infestation treatment (C=control, A=infested)

Sheet "PAP_highres": Flower traits

Data in this table comprises plant stress-related traits and floral traits measured per plant individual. Several traits were strongly correlated and therefore summarized into a single response (i.e., a single column) and modelled using a categorical co-predictor representing the respective pooled trait types.

• co-predictor - E_APHIDSIZE: size category of aphids (L=large,M=medium,S=small; see publication for explanation)

• response - NO_APHID: number of aphids of the regarding size per individual

• co-predictor - E_TFLOBUD: time point of data acquisition (days after aphid infestations) for the number of open flowers and flower buds

• response - NO_FLOBUD: number of open flowers and flower buds per individual

• co-predictor - E_COMPBIOM: component of dry biomass assessed (3shoot=shoot, 2flowers=all flowers and flower buds, 1siflo=single flower)

• response - BIOM: dry biomass (g) of a given component per individual

• co-predictor - E_SHAPETYPE: flower component for which area has been acquired (1egg=are covered by smallest possible circle drawn around flower, 2flo=all petals of a single flower, 3pet=area of one petal)

• response - SHAPE_AREA: display area (pixel)

• additional random factor - E_COL_TRIAL: flower colours have been assessed during different time points = trials (T1-T3)

• co-predictor - E_COLTYPE: wavelength range, i.e., quality of reflected light (2G=green, 3B=blue, 4U=UV)

• response - COL: incoming light reflected by flower (%)

• response - NECTAR_VOLUME: volume of nectar per flower (µL), note that this was measured repeatedly

Sheet "PAP_LSA_joint": Leaf pigments

Data in this table includes measurements of leaf light absorbance with an LSA device

• E_TIME: time point of data acquisition (1before=before aphid infestation, 2after=after aphid infestation)

• AFLAV: absorbance by epidermally located flavonoids (proportion)

• AANTH: absorbance by epidermally located anthocyanins (proportion)

• NMOL: chlorophyll content (nmol)

Sheet "PAP_choice": Binary choice tests with Hadena bicruris

• E_TEST: Type of choice test (CHOICETMT=effect of aphid infestation treatment within female and male plants, CHOICETMT=effect of plant sex within control and infested plants)

• ID_MOTH: identity of Hadena bicruris moth individual

• ID_PLANT_I: identity of choice plant 1

• ID_PLANT_II: identity of choice plant 2

• SP1: sex of choice plant 1 (F=female, M=male)

• SP2: sex of choice plant 2 (F=female, M=male)

• TP1: aphid infestation treatment of choice plant 1 (C=control, T=infested)

• TP2: aphid infestation treatment of choice plant 2 (C=control, T=infested)

• MATCH: columns ID_MOTH - TP2 merged

• E_TRIAL_ID: Trial identity (same for all plants with identical entries for MATCH)

• SP: sex choice plant 1 and choice plant 2 (F=female, M=male)

• TP: aphid infestation treatment choice plant 1 and choice plant 2 (C=control, T=infested)

• VISIT: visit number (1-4) in total, max four visits per trial were analysed

• E_CHOICE: behavioural parameter under observation (1vis=visited[1=visited, 0=not visited], 2fed=feeding[1=fed,0=not fed], 3time=time of entire visit [s])

• CHOICE1: behavioural parameter for a given trial and visit observed for plant 1

• CHOICE2: behavioural parameter for a given visit and trial observed for plant 2

Sheet "Scent_metabolome": Relative intensities (intensity of individual compound/intensity of internal standard) of floral volatile organic compounds (VOC) in Silene latifolia

• C01-C42 relative intensities of compound IDs C01-C42 (IDs correspond to metabolite identities in Supplementary Table S1A)

Sheet "Volcano_scent": Dataset for generation of volcano plots for floral scent composition in Silene latifolia

• metabolite ID: compound ID (IDs correspond to metabolite identities in Supplementary Table S1A)

• log2FC: log2(relative compound intensity infested/relative compound intensity control)

• pval=p-value uncorrected

• pvalcorr=p-value FDR corrected

Sheet "Nectar_metabolome": Intensities of metabolites in the nectar of Silene latifolia

• ID0001- ID4774 intensities of compound IDs ID0001- ID4774 (IDs correspond to metabolite identities in Supplementary Table S1B)

Sheet "Volcano_nectar ": Dataset for generation of volcano plots for floral nectar composition in Silene latifolia

• ID: compound ID (IDs correspond to metabolite identities in Supplementary Table S1A)

• mean_F: mean intensity in female plants

• mean_M: mean intensity in male plants

• mean_C: mean intensity in control plants

• mean_A: mean intensity in aphid-infested plants

• mean_FC: mean intensity in female control plants

• mean_FA: mean intensity in female-infested plants

• mean_MC: mean intensity in male control plants

• mean_MA: mean intensity in male-infested plants

• log2_F-M: log2(mean intensity female/mean intensity male)

• log2_A-C: log2(mean intensity infested/mean intensity control)

• log2_FA-FC: log2(mean intensity infested female/mean intensity control female)

• log2_MA-MC: log2(mean intensity infested male/mean intensity control male)

• log2_FC-MC: log2(mean intensity control female/mean intensity control male)

• log2_FA-MA: log2(mean intensity infested female/mean intensity infested male)

• OUTCOME CHECKING: outcome of best model loop (UNT=untransformed response, SQRT=square root transformed response, LOG=log10 transformed response)

• pun_F-M: uncorrected p-value sex effect

• pcorr_F-M: FDR corrected p-value sex effect

• mod_F-M: modulation sex effect (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])

• pun_A-C: uncorrected p-value aphid infestation effect

• pcorr_A-C: FDR corrected p-value aphid infestation effect

• mod_A-C: modulation aphid infestation effect (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])

• pun_FA-FC: uncorrected p-value aphid infestation effect within female plants

• pcorr_FA-FC: FDR corrected p-value of aphid infestation effect within female plants

• mod_FA-FC: modulation aphid infestation effect within female plants (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])

• pun_MA-MC: uncorrected p-value of aphid infestation effect within male plants

• pcorr_MA-MC: FDR corrected p-value of aphid infestation effect within male plants

• mod_MA-MC: modulation aphid infestation effect within male plants (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])

• pun_FC-MC: uncorrected p-value sex effect within control plants

• pcorr_FC-MC: FDR corrected p-value sex effect within control plants

• mod_FC-MC: modulation sex effect within control plants (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])

• pun_FA-MA: uncorrected p-value sex effect within infested plants

• pcorr_FA-MA: FDR corrected p-value sex effect within infested plants

• mod_FA-MA: : modulation sex effect within infested plants (un=not modulated [log2FC<1/>-1 & uncorrected p-value >0.05], mod =modulated [log2FC>1/<-1 & uncorrected p-value <0.05], modfd=modulated [log2FC>1/<-1 & FDR corrected p-value <0.05])
   

Code/software

None

Access information

Other publicly accessible locations of the data:

• Not applicable

Data was derived from the following sources:

• Experiment published in 10.1093/jxb/eraf522