https://doi.org/10.5061/dryad.905qfttvz

Description of the data and file structure

Figure 1 - includes plaque assay (PFU) data; quantification of microscopy images for the number of bacteria per cell, and bacterial length measurements from microscopy images.

Figure 2: Contains quantification of microscopy images for a number of bacteria associated with actin and quantification of bacteria from PFU assays.

Figure 3: Contains quantification of bacterial abundance (PFU assays), cell death, IFN-I production, and NF-kB activity assay data.

Figure 4: Contains % colocalization data of bacteria with ubiquitin; PFU data in BMDMs for ATG5-/- cells, and PFU data upon addition of 3MA inhibitor

Figure 5: Contains PFU data in BMDMs; contains quantification of microscopy images for chained bacteria; contains fold change of ROS; and PFU data upon addition of L-NIL

Sup Fig. 1: Measurement of bacterial abundance (PFU assays).

Sup Fig. 2: Measurement of bacterial abundance (PFU assays).

Sup Fig. 4: contains P value data

Files and variables

File: Source_Data_Sun_et_al_Nat_Communications_for_repository.xlsx

Description: The Excel file has tabs for each figure. The figures are described below. GSH=Glutathione; R=Rickettsia

Variables

• Fig. 1: GSH is required for cell division and growth of R. parkeri in epithelial cells.
• Fig. 2: Host GSH is required for proper actin-based motility and cell-to-cell spread of R. parkeri
• Fig. 3: Host GSH is critical for R. parkeri survival in macrophages via a non-bacteriolytic restriction mechanism.
• Fig. 4: Host GSH is required for R. parkeri to avoid ubiquitylation and antibacterial autophagy in macrophages.
• Fig. 5: GSH serves as a cysteine source for R. parkeri cell division and survival.
• Supplementary Figure 1: GSH, CysGly and cystine do not rescue survival or chaining defects of *R. parkeri in BSO-treated cells
• Supplementary Figure 2: Inhibiting iNOS does not rescue R. parkeri survival upon BSO treatment in the absence or presence of ATG5.
• Supplementary Figure 4: p value for all figures.

Abbreviations and units of measure:

• BSO = buthionine sulfoximine
• Rp or R. parkeri = Rickettsia parkeri
• GSH = glutathione
• h = hour, hpi = hours postinfection, d = day, dpi = days postinfection
• tail refers to actin tails
• LDH = lactate dehydrogenase
• BMDM = bone marrow-derived macrophage
• RLUs = relative light units
• 3MA = 3 methyl adenine
• NAC = N-acetylcysteine
• GSHee = glutathione ethyl ester
• L-NIL is an inhibitor of inducible nitric oxide synthase (iNOS)
• Cys-Gly = cysteinyl glycine

Code/software

No software or unique code is needed to analyze the data

Access information

Other publicly accessible locations of the data:

• NA

Data was derived from the following sources:

• NA