Dataset DOI: 10.5061/dryad.905qfttx3

Description of the data and file structure

These experiments were conducted to assess antibody responses (binding, functional, and neutralizing) against SARS-CoV-2 in 2020-2021 in New Mexican patients and to assess the effect of vaccination on pre-existing antibody titers. All missing values are represented as N/A.

Files and variables

File: Figure_1_data.xlsx

Description: 

• Column A:  patient number
• Column B:  ELISA titers agaSARS-CoV-2CoV-2 from the first blood draw of unvaccinated patients
• Column C:  ELISA titers againSARS-CoV-2V-2 from the second blood draw of unvaccinated patients
• Column D:  ELISA titers agaSARS-CoV-2CoV-2 from the first blood draw of unvaccinated patients that were later vaccinated
• Column E:  ELISA titers against SARS-CoV-22 from the second blood draw of patients who were vaccinated between draws 1 and 2

IgG concentrations were calculated utilizing the trendline equation created using the standard’s included in the kit.

File: Figure_2_data_revised.xlsx

Description: 

• Column A:  patient number
• Column B:  Fold change in ELISA titers against SARS-CoV-2 between blood draws 1 and 2
• Column C:  Fold change in ELISA titers against adenovirus between blood draws 1 and 2
• Column D:  Fold change in ELISA titers against cytomegalovirus between blood draws 1 and 2
• Column E:  Fold change in ELISA titers against measles virus between blood draws 1 and 2
• Column F:  Fold change in ELISA titers against common cold coronavirus hCoV 299E between blood draws 1 and 2

Fold change in IgG concentration between draw 1 and draw 2 was calculated for each patient.

File: Figure_3_data_revised.xlsx

Description: 

• Column A:  patient number
• Column B:  neutralizing antibody titers agaiSARS-CoV-2oV-2 from the first blood draw of unvaccinated patients
• Column C:  neutralizing antibody titers agaSARS-CoV-2CoV-2 from the second blood draw of unvaccinated patients
• Column D:  neutralizing antibody titers against agSARS-CoV-2 CoV-2 from the first blood draw of unvaccinated patients that were later vaccinated
• Column E:  neutralizing antibody titers against agSARS-CoV-2 CoV-2 from the second blood draw of patients who were vaccinated between draws 1 and 2

Neutralization of SARS-CoV-2 live virus was assessed through a PRNT.

File: Figure_4_data_revised.xlsx

Description: 

• Column A:  patient number
• Column B:  antibody-dependent cell-mediated cytotoxicity (ADCC) titers agaiSARS-CoV-2oV-2 from the first blood draw of unvaccinated patients
• Column C:  antibody-dependent cell-mediated cytotoxicity titers against againsSARS-CoV-2-2 from the second blood draw of unvaccinated patients
• Column D:  antibody-dependent cell-mediated cytotoxicity titers against SARS-CoV-22 from the first blood draw of unvaccinated patients who were later vaccinated
• Column E:  antibody-dependent cell-mediated cytotoxicity titers against agSARS-CoV-2 CoV-2 from the second blood draw of patients who were vaccinated between draws 1 and 2

ADCC activity was assessed using an ADCC luciferase reporter assay.

Code/software

Data are Excel worksheets

Access information

Other publicly accessible locations of the data:

• None

Data was derived from the following sources:

• None

Human subjects data

We received explicit informed consent from the study participants to publish de-identified data in the public domain. The data was de-identified by blinding experimentalists from PHI and assigning a anonymized number to each patient.