Dataset DOI: 10.5061/dryad.95x69p8z9

Description of the data and file structure

This dataset includes additional data collected and included in figures in Bennett et. al. 2025.  Data was compiled using GraphPad Prism software and Living Image Software and is available in the following files.

This txt files are metadata from a biomedical imaging experiment performed using the Living Image with an IVIS Spectrum CT system. It records the technical settings and conditions used to capture luminescent images of a mouse, such as exposure time, camera temperature, filters, and calibration parameters. The actual images (e.g., photograph.TIF and luminescent. TIF) contain the visual results, while this file describes how those images were acquired and processed. It is commonly used in bioluminescence or fluorescence imaging studies in biomedical research.

• Figure 1. Three days post-infection, mice were anesthetized and imaged with the Xenogen Spectrum CT. The average radiance per region of interest (ROI) drawn was quantified and compared between groups via a one-way ANOVA with Holm-Šídák's multiple comparisons test. ns=not significant; ***p<0.001; ****p<0.0001
  • SequenceInfo_Figure_1.txt
  • Supporting_Data-Figure_1_and_2_and_Supplement_1_and_2.prism
• Figure 2. Every 3-13 days post-infection,n micm from Figure 1 were anesthetized and imaged with the Xenogen Spectrum CT. The data are represented as the average radiance and standard deviation per group at each time point. Probability of survival, median survival time, and difference in survival were calculated using GraphPad Prism 10. Survival curves were compared by a Log-rank (Mantel-Cox) test. ns=not significant; **p<0.01; ***p<0.001; ****p<0.0001
  • Supporting_Data-Figure_1_and_2_and_Supplement_1_and_2.prism
• Supplemental Figure 1. Seven, eleven, and fourteen days post-infection, mice from Figure 1 were anesthetized and imaged with the Xenogen Spectrum CT. The average radiance per ROI was quantified and compared between groups via a one-way ANOVA with Holm-Šídák's multiple comparisons test. ns=not significant; *p<0.05; ****p<0.0001
  • Supporting_Data-Figure_1_and_2_and_Supplement_1_and_2.prism
  • SequenceInfo_Supplement_1A.txt
  • SequenceInfo_Supplement_1B.txt
  • SequenceInfo_Supplement_1C.txt
• Supplemental Figure 2. Twenty,  twenty-seven, thirty-four, forty-seven, and sixty-five days post-infection, female mice from Figure 1 were anesthetized and imaged with the Xenogen Spectrum CT. The average radiance per ROI was quantified and compared between groups via a two-tailed, unpaired t-test. ns=not significant
  • Supporting_Data-Figure_1_and_2_and_Supplement_1_and_2.prism
  • SequenceInfo_Supplement_2A.txt
  • SequenceInfo_Supplement_2B.txt
  • SequenceInfo_Supplement_2C.txt
  • SequenceInfo_Supplement_2D.txt
  • SequenceInfo_Supplement_2E.txt
• Figure 3. Peripheral blood was collected from individual mice at 4- and 11-days post-infection (dpi) via the facial vein, and at 21 days post-infection (dpi) via a post-mortem cardiac puncture. Absolute white blood cell count and per cell type per µL of blood (cells/µL) was calculated by counting the number of neutrophils, lymphocytes, monocytes, and eosinophils in ten microscopic fields per slide at 4 dpi, 11 dpi, and  21 dpi. Data represent the average and standard deviation of cells/µL per group. Average cells/µL are compared between diet-sex groups, using a two-way-ANOVA with Tukey’s multiple comparisons test. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001
  • Supporting_Data-_Figure_3.prism
• Figure 4. Using the LEGENDplex™ Mouse Inflammation panel, serum concentrations of IL-23, IL-1α, IFN-γ, TNF-α, MCP-1, IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A, IFN-β, GM-CSF of the mice in Figure 3. Cytokine concentrations are compared between diet-sex cohorts within the same day post-infection, as well as individual diet-sex cohorts between days post-infection, using Mixed-effects analysis with Tukey's multiple comparisons test. *p<0.05; **p<0.01
  • Supporting_Data-_Figure_4.prism
• Figure 5. Using the LEGENDplex™ Mouse Inflammation panel, serum concentrations of IL-23, IL-1α, IFN-γ, TNF-α, MCP-1, IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A, IFN-β, and GM-CSF of infected mice euthanized at 11 days post-infection. Cytokine concentrations are compared between diet-sex cohorts within the same day post-infection, as well as individual diet-sex cohorts between days post-infection, using Mixed-effects analysis with Tukey's multiple comparisons test. *p<0.05; **p<0.01
  • Supporting_Data-Figure_5.prism
• Supplemental Figure 3. On day 9 post-infection,n mice were euthanized, ed and the gallbladder, liver, spleen, cecum, and whole blood of infected mice were collected, and sections of each tissue were homogenized to enumerate CFUs per mg of tissue. Log₁₀CFU/mg were compared between groups by a two-way ANOVA with uncorrected Fisher’s LSD test. Probability of survival, median survival time, and difference in survival were calculated using GraphPad Prism 10. Survival curves were compared by a Log-rank (Mantel-Cox) test,t and all comparisons and results are indicated in the figure legend. ns=not significant; *p<0.05; **p<0.01
  • Supporting_Data-_Supplemental_Figure_3.prism
• Supplemental Figure 4. Eleven days post-infection, mice from Figure 5 were euthanized, and the gallbladder, liver, spleen, cecum, and whole blood of S. Typhimurium (STm) infected mice were collected, and sections of each tissue were homogenized to enumerate CFUs per mg of tissue. Log₁₀CFU/mg were compared between groups by a one-way ANOVA with Tukey’s multiple comparisons test. Serum IFNγ, TNF, and IL-10 cytokine levels were measured individually by ELISA (n= 3-5). Cytokine levels were compared between groups by a two-way ANOVA with uncorrected Fisher’s LSD test. ns=not significant; *p<0.05; **p<0.01
  • Supporting_Data-_Supplemental_Figure_4.prism

Additional explanation of data sources and analysis techniques can be found within each file.