Copulatory wounding in Drosophila melanogaster
Data files
May 14, 2025 version files 288.29 KB
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cop_wound.R
59.04 KB
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corr.csv
565 B
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day136.csv
814 B
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lab_experiment1.csv
56.59 KB
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lab_experiment2.csv
143.49 KB
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lab_lat_dur1.csv
3.09 KB
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lab_lat_dur2.csv
1.16 KB
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lateral_folds.csv
826 B
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README.md
17.02 KB
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repeat.csv
803 B
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wild_collected.csv
4.89 KB
Abstract
Copulation can result in males inflicting wounds to the female genitalia, so-called traumatic mating. Such wounds are potentially costly as they could be entry points for infections, and they have been associated with a shorter lifespan in insect species. In many species of insects, females mate with more than one male, which leads to the question of whether the number of matings affects the amount of genital damage that females suffer from those matings. Here we test whether copulation frequency affects the number or size of genital wounds in Drosophila melanogaster. Females who mated twice had more genital wounds and a larger total area of wounding, compared to females who mated once. However, females who refused to mate a second time had a similar area of wounding as females who mated twice. We found that wounds to the ventral abdomen also increased with increased mating frequency. Our results show that polyandry can result in increased female copulatory wounding in this species. The extent to which the wounds are costly for females is currently unknown. Investigating genital and abdominal wounds is crucial to a better understanding of the consequences of sexual conflict and the selective pressures shaping mating behaviour.
This cop_wound_readme.txt file was generated on 2024-02-06 by SOPHIE ARMITAGE.
GENERAL INFORMATION
- Title of Datasets: cop_wound_dataset
- Author Information
Principal Investigator Contact Information
Name: Sophie Armitage
Institution: Freie Universität Berlin
Address: Königin-Luise-Str. 1-3, 14195 Berlin, Germany
Email: sophie.armitage@fu-berlin.de - Date of data collection: June 2021 to October 2024.
- Geographic location of data collection: Berlin and Brandenburg, Germany
- Information about funding sources that supported the collection of the data: SAOA was supported by a Heisenberg Fellowship (AR 872/4-1 and AR 872/7-1) from the Deutsche Forschungsgemeinschaft (DFG; https://www.dfg.de/en/index.jsp). BSS was supported by the DFG through project AR 872/5-1, awarded to SAOA as part of the Research Unit FOR 5026 “InsectInfect”.
SHARING/ACCESS INFORMATION
- Licenses/restrictions placed on the data: No
- Links to publications that cite or use the data: Subasi, Finsterbusch, Büge & Armitage (2024). Two matings lead to more copulatory wounding than a single mating in female Drosophila melanogaster. DOI not available at the time of submission of these data.
- Was data derived from another source? no
- Recommended citation for this dataset:
DATA & FILE OVERVIEW
- File List:
cop_wound.R The R code required to process the data in the csv files and produce the data figures for the study, and carry out statistical analyses.
corr.csv Area repeated measures for correlations.
lateral_folds.csv Area of wounds on the vaginal furcal dorsolateral folds, recorded from either one side or both sides of the same samples.
day136.csv Area of wounds over days 1, 3, and 6 post-mating.
lab_experiment1.csv Number of copulations, mating latency, copulation duration, and wounding of individual female flies for experiment 1.
lab_experiment2.csv Number of copulations, mating latency, copulation duration, and wounding of individual female flies for experiment 2.
lab_lat_dur1.csv Subset of data from lab_experiment1.csv, containing only females who mated twice.
lab_lat_dur2.csv Subset of data from lab_experiment2.csv, containing only females who mated twice.
repeat.csv Repeated measures of area to calculate methodological repeatability.
wild_collected.csv Presence and absence of offspring, and presence of wounding to lateral folds in wild-collected D. melanogaster.
- Relationship between files, if important:
corr.csv and repeat.csv contain data from the same set of flies.
lab_lat_dur1.csv contains a subset of data from lab_experiment1.csv.
lab_lat_dur2.csv contains a subset of data from lab_experiment2.csv. - Are there multiple versions of the dataset? no
DATA-SPECIFIC INFORMATION FOR: corr.csv
- Number of variables: 3
- Number of cases/rows: 35
- Variable List:
Each row contains data for an individual fly and indicates repeated measures (repeated analyses) of the photographs of the area of wounding.
female_id Individual identity of the fly
area Area of wounding (micrometers^2)
area2 Repeated measurement of the area of wounding
DATA-SPECIFIC INFORMATION FOR: lateral_folds.csv
- Number of variables: 7
- Number of cases/rows: 12
- Variable List:
Each row contains data for an individual fly and indicates repeated measures (repeated analyses) of the photographs of the area of wounding.
female_id Individual identity of the fly, repeated numbers were used across experiments
experiment The experiment was repeated three times, indicated by a, b, and c.
combined_id The individual identity of the fly, combined with the experimental replicate. Each value is unique.
population Whether the female came from a population deriving from Germany (de) or Portugal (pt).
treatment_full The full names of the treatment groups, where 1_mating = female mated once, 2_ mating = female mated twice, 1_virgin = unmated females dissected at the same time as the first mated females, 2_virgin = unmated females dissected at the same time as the second mated females.
area_2_folds Area measurements of wounds on the vaginal furcal dorsolateral folds, recorded from both sides.
area_1_fold Area measurements of wounds on the vaginal furcal dorsolateral folds, recorded from one side.
DATA-SPECIFIC INFORMATION FOR: day136.csv
- Number of variables: 3
- Number of cases/rows: 57
- Variable List:
Each row contains data for an individual fly and indicates the day after mating at which she was dissected, and the area of wounding.
female_id Individual identity of the fly
day_to_sample Day after mating at which the female was dissected
area Area of wounding (micrometers^2)
DATA-SPECIFIC INFORMATION FOR: lab_experiment1.csv
- Number of variables: 24
- Number of cases/rows: 808
- Variable List:
Each row contains data for an individual fly and indicates the number of times the female copulated, mating latency, copulation duration, and wounding.
female_id Individual identity of the fly, repeated numbers were used across experiments
experiment The experiment was repeated three times, indicated by a, b, and c.
combined_id The individual identity of the fly, combined with the experimental replicate. Each value is unique.
population Whether the female came from a population deriving from Germany (de) or Portugal (pt).
treatment_matings The treatment group that a female was assigned to, where 0 = virgin, 1 = one mating, 2 = two matings.
treatment_full The full names of the treatment groups, where 1_mating = female mated once, 2_ mating = female mated twice, 1_virgin = unmated females dissected at the same time as the first mated females, 2_virgin = unmated females dissected at the same time as the second mated females.
latency_mins_1 The length of time (in minutes) before the first mating started after the introduction of the male to the vial containing the female.
duration_mins_1 The duration of the first mating, in minutes.
mating_succ_1 1 = the first mating was successful, 0 = the first mating was unsuccessful.
dur_under_10_mins_1 The copulation duration of the first mating lasted for less than 10 minutes.
larvae_mating_1 1 = larvae were produced after the first mating, 0 = larvae were not produced
larvae_mating_1_fig Used to make a subset to plot figure 2A and C. 1 = larvae were produced after the first mating, plus the virgins which are NA in the column larave_mating_1, 0 = larvae were not produced
latency_mins_2 The length of time (in minutes) before the second mating started after the introduction of the male to the vial containing the female.
duration_mins_2 The duration of the second mating, in minutes.
mating_succ_2 1 = the second mating was successful, 0 = the second mating was unsuccessful.
dur_under_10_mins_2 The copulation duration of the second mating lasted for less than 10 minutes.
wounding_fold 1 = female had visible wounding, 0 = female had no visible wounding.
wound_area Area of wounding (micrometers^2).
wound_number Number of visible genital wounds.
mate1_area 1 = females who mated once and area data are available, 0 = all other females.
mate1_numb_wounds 1 = females who mated once, and the number of wounds data are available, 0 = all other females.
mate12_area 1 = females who mated twice and area data are available, 0 = all other females.
mate12_numb_wounds 1 = females who mated twice, and the number of wounds data are available, 0 = all other females.
wounding_abdomen 1 = black, putatively melanised marks are visible on the external ventral abdomen, 0 = black marks not visible.
- Missing data codes:
Missing data are denoted as “NA”
DATA-SPECIFIC INFORMATION FOR: lab_experiment2.csv
- Number of variables: 50
- Number of cases/rows: 892
- Variable List:
Each row contains data for an individual fly and indicates the number of times the female copulated, mating latency, copulation duration, and wounding.
female_id Individual identity of the fly, repeated numbers were used across experiments
experiment The experiment was repeated three times, indicated by a, b, and c.
combined_id The individual identity of the fly, combined with the experimental replicate. Each value is unique.
treatment_matings The treatment group that a female was assigned to, where 0 = virgin, 1 = one mating, 2 = two matings.
treatment_full The full names of the treatment groups, where virgin_day5 = unmated females who were not allowed to mate on day five, virgin_day7 = unmated females who were not allowed to mate on day seven, 1_mating_day5 = female mated once on day five, 1_mating_day7 = female mated once on day seven, 2_mating_day5_7_refused = females mated on day five but who did not remate on day seven despite being exposed to a male, 2_ mating_day5_7 = double mated females who mated once on day five and again on day seven.
latency_mins_1 The length of time (in minutes) before the first mating started after the introduction of the male to the vial containing the female.
duration_mins_1 The duration of the first mating, in minutes.
dur_under_10_mins_1 The copulation duration of the first mating lasted for less than 10 minutes.
mating_succ_1 1 = the first mating was successful, 0 = the first mating was unsuccessful.
larvae_mating_1 1 = larvae were produced after the first mating, 0 = larvae were not produced
latency_mins_2 The length of time (in minutes) before the second mating started after the introduction of the male to the vial containing the female.
duration_mins_2 The duration of the second mating, in minutes.
dur_under_10_mins_2 The copulation duration of the second mating lasted for less than 10 minutes.
mating_succ_2 1 = the second mating was successful, 0 = the second mating was unsuccessful.
number_genital_wounds Number of visible genital wounds.
exclude 1 = samples excluded from the analysis, 0 = samples not excluded from the analysis.
wounding_fold 1 = female had visible wounding, 0 = female had no visible wounding.
wound_area Area of wounding (micrometers^2).
wounding_abdomen_before_mating 1 = female had visible wounding on abdomen before mating, 0 = female had no visible wounding on abdomen before mating.
wounding_abdomen_after_mating 1 = female had visible wounding on abdomen after mating, 0 = female had no visible wounding on abdomen before mating.
number_wounding_abdomen_before_mating Number of visible abdominal wounds before mating.
number_wounding_abdomen_after_mating Number of visible abdominal wounds after mating.
place_wounding_abdomen_after_mating_L_A1 1 = female had visible wounding on the left first abdominal segment after mating, 0 = female had no visible wounding on the left first abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A2 1 = female had visible wounding on the left second abdominal segment after mating, 0 = female had no visible wounding on the left second abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A3 1 = female had visible wounding on the left third abdominal segment after mating, 0 = female had no visible wounding on the left third abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A4 1 = female had visible wounding on the left fourth abdominal segment after mating, 0 = female had no visible wounding on the left fourth abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A5 1 = female had visible wounding on the left fifth abdominal segment after mating, 0 = female had no visible wounding on the left fifth abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A6 1 = female had visible wounding on the left sixth abdominal segment after mating, 0 = female had no visible wounding on the left sixth abdominal segment after mating.
place_wounding_abdomen_after_mating_L_A7 1 = female had visible wounding on the left seventh abdominal segment after mating, 0 = female had no visible wounding on the left seventh abdominal segment after mating.
place_wounding_abdomen_after_mating_left 1 = female had visible wounding on the left side of the abdomen after mating, 0 = female had no visible wounding on the left side of the abdomen after mating.
place_wounding_abdomen_after_mating_R_A1 1 = female had visible wounding on the right first abdominal segment after mating, 0 = female had no visible wounding on the right first abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A2 1 = female had visible wounding on the right second abdominal segment after mating, 0 = female had no visible wounding on the right second abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A3 1 = female had visible wounding on the right third abdominal segment after mating, 0 = female had no visible wounding on the right third abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A4 1 = female had visible wounding on the right fourth abdominal segment after mating, 0 = female had no visible wounding on the right fourth abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A5 1 = female had visible wounding on the right fifth abdominal segment after mating, 0 = female had no visible wounding on the right fifth abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A6 1 = female had visible wounding on the right sixth abdominal segment after mating, 0 = female had no visible wounding on the right sixth abdominal segment after mating.
place_wounding_abdomen_after_mating_R_A7 1 = female had visible wounding on the right seventh abdominal segment after mating, 0 = female had no visible wounding on the right seventh abdominal segment after mating.
place_wounding_abdomen_after_mating_right 1 = female had visible wounding on the right side of the abdomen after mating, 0 = female had no visible wounding on the right side of the abdomen after mating.
- Missing data codes:
Missing data are denoted as “NA”
DATA-SPECIFIC INFORMATION FOR: lab_lat_dur1.csv
- Number of variables: 7
- Number of cases/rows: 112
- Variable List:
Subset of data from lab_experiment.csv, containing only females who mated twice. Rows contain data for individual flies, and each fly has two rows.
female_id Individual identity of the fly
experiment The experiment was repeated three times, indicated by a, b, and c.
combined_id The individual identity of the fly, combined with the experimental replicate. Each value is unique.
population Whether the female came from a population deriving from Germany (de) or Portugal (pt).
first_or_second_mating Whether the data is from the females' first or second mating.
latency_mins The length of time (in minutes) before mating started after the introduction of the male to the vial containing the female.
duration_mins The duration of the mating, in minutes.
DATA-SPECIFIC INFORMATION FOR: lab_lat_dur2.csv
- Number of variables: 6
- Number of cases/rows: 44
- Variable List:
Subset of data from lab_experiment2.csv, containing only females who mated twice. Rows contain data for individual flies, and each fly has two rows.
female_id Individual identity of the fly
experiment The experiment was repeated three times, indicated by a, b, and c.
combined_id The individual identity of the fly, combined with the experimental replicate. Each value is unique.
first_or_second_mating Whether the data is from the females' first or second mating.
latency_mins The length of time (in minutes) before mating started after the introduction of the male to the vial containing the female.
duration_mins The duration of the mating, in minutes.
DATA-SPECIFIC INFORMATION FOR: repeat.csv
- Number of variables: 2
- Number of cases/rows: 70
- Variable List:
Repeated measures of area are used to calculate methodological repeatability. Rows contain data for individual flies, and each fly has two rows, i.e., measurements were repeated twice for each fly.
female_id Individual identity of the fly
area Area of wounding (micrometers^2).
DATA-SPECIFIC INFORMATION FOR: wild_collected.csv
- Number of variables: 7
- Number of cases/rows: 105
- Variable List:
Presence and absence of offspring, and presence of wounding to lateral folds in wild-collected D. melanogaster. Each row contains data for one fly.
species Dmel = Drosophila melanogaster, Dsim = Drosophila simulans.
season Season in which the fly was collected.
site Collection site.
date_collected Date on which the fly was collected from the wild.
sample_id Individual sample identification of each female
offspring 1 = female produced offspring, 0 = female did not produce offspring
wounding_fold 1 = female had a wounded lateral fold, 0 = female had no visible wounding.
The data were obtained from wild-collected flies and experiments that were carried out in the lab. For full details, see the methods described in Subasi, Finsterbusch, Büge & Armitage: "Two matings lead to more copulatory wounding than a single mating in female Drosophila melanogaster."
Briefly, the fly and data collection were carried out in the methods described in Subasi, Finsterbusch, Büge & Armitage (2024). Females mating twice or refusing to remate receive more copulatory wounding than singly mated females. At data entry, the data were double-checked for accuracy.