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Electrophysiology and anatomy data for: Projection-specific integration of convergent thalamic and retrosplenial signals in the presubicular head direction cortex

Richevaux, Louis1; Lim, Dongkyun1; Nassar, Mérie1; Dias Rodrigues, Léa1; Mauthe, Constanze1; Cohen, Ivan2; Sol-Foulon, Nathalie2; Fricker, Desdemona1

Research facility: Université Paris Cité
Published Dec 24, 2025 on Dryad. https://doi.org/10.5061/dryad.9ghx3ffxd

Data files

Dec 24, 2025 version files 3.77 GB

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Abstract

Head-direction (HD) signals function as the brain’s internal compass. They are organized as an attractor and anchor to the environment via visual landmarks. Here, we examine how thalamic HD signals and visual information from the retrosplenial cortex combine in the presubiculum. We find that monosynaptic excitatory connections from the anterior thalamic nucleus and from retrosplenial cortex converge on single-layer 3 pyramidal neurons in the dorsal portion of the mouse presubiculum. Independent dual wavelength photostimulation of these inputs in slices leads to action potential generation preferentially for near-coincident inputs, indicating that layer 3 neurons can transmit a visually matched HD signal to the medial entorhinal cortex. Layer 4 neurons, which innervate the lateral mammillary nucleus, form a second step in the association of HD and landmark signals. They receive little direct input from thalamic and retrosplenial axons. We show that layer 4 cells are excited di-synaptically, transforming regular spiking activity into bursts of action potentials, and that their firing is enhanced by cholinergic agonists. Thus, a coherent sense of orientation involves projection-specific translaminar processing in the presubiculum, where neuromodulation facilitates landmark updating of HD signals in the lateral mammillary nucleus.