Aedes sierrensis - Lambornella clarki host - parasite interaction data: Field survey and temperature dependence laboratory experiments
Data files
Nov 21, 2025 version files 794.36 KB
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GEE_ExtractPRISM_FieldSurvey_Ecol.rtf
65.99 KB
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GEE_ExtractPRISM_iButtons_Ecol.rtf
31.74 KB
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README.md
7.96 KB
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TreeHoles_2022InfectionTemperatureExperiment_Ecol.csv
57.64 KB
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TreeHoles_FieldSurvey_InfectionData_Ecol.csv
37.21 KB
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TreeHoles_ibuttons_forGEE_Ecol.csv
2.82 KB
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TreeHoles_Ibuttons_Infection_Ecol.csv
446.99 KB
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TreeHoles_Ibuttons_PRISM_Comparison_Ecol.R
4.13 KB
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TreeHoles_InfectionExperiment_PopulationTPCs_Ecol.R
54.88 KB
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TreeHoles_InfectionFieldSurvey_Analysis_Final_Ecol.R
9.11 KB
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TreeHoles_SampleDates_LatLon_Ecol.csv
34.13 KB
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TreeHoles_TPCs_OverallInteraction_Ecol.R
33.30 KB
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TreeHolesTemperature_InfectionExperimentTimeline_Ecol.R
4.82 KB
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TreeHolesTemperature_LambornellaFreeLivingExperiment_Ecol.csv
1.29 KB
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TreeHolesTemperature_MelanizationAssay_Ecol.csv
2.35 KB
Abstract
Temperature is a fundamental driver of species interactions, including parasitism. Here, we use field surveys and lab experiments to characterize the temperature dependence of the interaction between the mosquito host Aedes sierrensis and its facultative ciliate parasite Lambornella clarki. Field survey data quantifying infection presence across a 1100 km geographic climate gradient are paired with long-term environmental temperature measurements from PRISM. PRISM temperature estimates are compared to direct measurements of tree hole habitat temperatures logged with iButtons. Lab experiments with six host populations from different climates across the study area describe the fundamental temperature sensitivity of the host - parasite interaction and underlying host and parasite traits.
Dataset DOI: 10.5061/dryad.brv15dvkg
Description of the data and file structure
Field survey data were collected from sampled tree holes in California, Oregon, and Washington during the 2022 and 2023 rainy seasons. Ibutton field temperature data was logged during between 2022 and 2023 sampling visits. Climate and weather data are extracted from PRISM using Google Earth Engine.
Data from experiments were generated from incubation of Aedes sierrensis hosts and Lambornella clarki parasites at different temperatures in the laboratory.
Files and variables
File: TreeHolesTemperature_LambornellaFreeLivingExperiment_Ecol.csv
Description: Data from the free-living Lambornella clarki population growth experiment.
Variables
- Temperature: incubation temperature (ºC)
- Tray ID: identifier for the 6-well plate in which the microcosm was housed
- Well ID: identifier for the microcosm
- day0 – starting number of Lambornella clarki cells per 100 uL
- day1 – number of Lambornella clarki cells per 100 uL after one day of incubation
- day2 – number of Lambornella clarki cells per 100 uL after two days of incubation
- day3 – number of Lambornella clarki cells per 100 uL after three days of incubation
- day4 – number of Lambornella clarki cells per 100 uL after four days of incubation
- day5 – number of Lambornella clarki cells per 100 uL after five days of incubation
- replicate – replicate number for the temperature treatment
File: TreeHolesTemperature_MelanizationAssay_Ecol.csv
Description: Data from the melanization assay experiment.
Variables
- temperature – incubation temperature (ºC)
- tray – identifier for the 6-well plate in which the microcosm was housed
- well – identifier for the microcosm
- start.date – date on which the melanization response was stimulated
- time – time at which the melanization response was stimulated
- check.date – date on which the melanization response was assessed
- check.time – time at which the melanization response was assessed
- num.alive – number of larvae alive at the time of the melanization assessment (out of 5)
- num.melanized – number of larvae with a melanization spot at the puncture site
File: TreeHoles_2022InfectionTemperatureExperiment_Ecol.csv
Description: Data from the infection experiment.
“NA” represents that the field was not applicable, e.g., num.infections was NA when there were no surviving larvae left to inspect for infections
Variables
- temperature – incubation temperature (ºC)
- tray – identifier for the 6-well plate in which the microcosm was housed
- population – host population identifier
- well – identifier for the microcosm
- climate – climate category for the host population
- check.date – date of the microscope check
- check.day – day of the experiment on which the microscope check took place
- check.num – experimental checkpoint number
- num.alive – number of juvenile mosquitoes alive
- num.infected – number of ciliate infections observed
- num.melanized – number of larvae with melanization spots
- tot.melanized – cumulative larvae with melanization spots
- tot.infected – cumulative larvae with ciliate infections
- tot.dead – cumulative mosquito host deaths
- deaths.infected – cumulative deaths of infected hosts
- start.num – starting number of mosquito larvae in the microcosm
- last.check – binary, 1 indicates that the row contains the final observation data for the microcosm
- new.deaths.infected - count of new infection mortalities observed for the checkpoint
- lambo.count - density of free-living ciliates per 300 uL in the microcosm
File: TreeHoles_FieldSurvey_InfectionData_Ecol.csv
Description:
Variables
- treehole.id – identifier for each sampled tree hole
- Date.of.Sampling – date of sample collection
- num.checked – number of juvenile mosquitoes from the sample that were checked for ciliate infection and for melanization spots
- num.infected – number of infected mosquitoes observed in the sample
- num.melanized – number of mosquitoes with melanization spots observed in the sample
- th.infected – binary, 1 indicates infection presence in the sample
- th.melanized – binary, 1 indicates melanization spot presence in the sample
- lat – tree hole latitude (º)
- long – tree hole longitude (º)
File: TreeHoles_Ibuttons_Infection_Ecol.csv
Description: Data from iButton temperature loggers placed within tree holes
Variables
- treehole.id – identifier for the tree hole in which the iButton was placed
- date – date of the temperature data
- placementdate – date of iButton placement within the tree hole
- finaldate – date of iButton removal from the tree hole
- day_mean_temp_ib – the mean temperature recorded by the iButton on the date (ºC)
- infected – binary, 1 indicates that infected mosquitoes were collected from the tree hole at least once during the survey
File: TreeHoles_SampleDates_LatLon_Ecol.csv
Description: gps coordinates and sampling dates for the infection field survey, for use in Google Earth Engine with "GEE_ExtractPRISM_FieldSurvey_Ecol.rtf"
Variables
- latitude: surveyed tree hole latitude (º)
- longitude: surveyed tree hole longitude (º)
- sampledate: date on which tree hole was sampled
- surveyyear: year of sampling
- treeholeid: identifier for the sampled tree hole
File: TreeHoles_ibuttons_forGEE_Ecol.csv
Description: locations and dates for iButton logs of temperatures within tree hole habitats, for use in Google Earth Engine with "GEE_ExtractPRISM_iButtons_Ecol.rtf"
Variables
- finaldate: date on which iButton was removed from the tree hole
- ibutton: identifier number for the iButton
- latitude: latitude of the tree hole in which the iButton was placed (º)
- longitude: longitude of the tree hole in which the iButton was placed (º)
- placementdate: date on which the iButton was placed inside the tree hole
- treeholeid: identifier for the tree hole
Code/software
File: TreeHoles_iButtonsPRISM_comparison_Ecol.R
Description: R script for analyzing the paired field iButton and PRISM temperature data
File: TreeHoles_TPCs_OverallInteraction_Ecol.R
Description: R script fits and plots thermal performance curves from the infection experiment for the data combined across all host populations; for the free-living Lambornella populatoin growth rate experiment; and for the melanization assay experiment
File: TreeHoles_InfectionExperiment_PopulationTPCs_Ecol.R
Description: Fits and plots thermal performance curves from the infection experiment separately for each population
File: TreeHolesTemperature_InfectionExperimentTimeline_Ecol.R
Description: time series plots for the infection experiment
File: GEE_ExtractPRISM_FieldSurvey_Ecol.rtf
Description: Google Earth Engine script for extracting PRISM temperature and precipitation data used in the field survey analysis
File: TreeHoles_InfectionFieldSurvey_Analysis_Final_Ecol.R
Description: field survey infection data analysis
File: GEE_ExtractPRISM_iButtons_Ecol.rtf
Description: Google Earth Engine script for extracting PRISM daily mean temperature estimates for tree holes with iButton daily temperature time series
File: TreeHoles_Ibuttons_PRISM_Comparison_Ecol.R
Description: comparison of iButton and PRISM temperature data
Access information
Other publicly accessible locations of the data:
- Extracted PRISM data used in the analyses are available on Github: https://github.com/johannah-farner/TreeHoles_Ecol
There are five data files.
Two files contain field data for tree holes sampled in the Lambornella clarki infection survey. One of these files contains the infection data from the sampled juvenile Aedes sierrensis mosquitoes, plus temperature and precipitation data from PRISM and RADS-CHIRPS. The other file contains temperature data logged by iButtons placed within tree holes, plus temperature data from PRISM and infection presence data.
Three files contain data from the laboratory temperature dependence experiments. One file contains data from the infection experiment, one file contains data from the Lambornella clarki free-living population growth experiment, and one file contains data from the mosquito melanization assay.