The facultative intracellular symbiont Lariskella is neutral for lifetime fitness and spreads through cytoplasmic incompatibility in the leaffooted bug, Leptoglossus zonatus
Data files
May 03, 2025 version files 30.15 KB
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EFU_Dryad_pkg.zip
22.32 KB
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README.md
7.83 KB
Abstract
To examine the effects of the intracellular symbiont Lariskella on bug performance and reproduction as well as in possible interactions with the bug’s obligate nutritional symbiont, Caballeronia, Leptoglossus zonatus individuals were reared in a factorial experiment with both Lariskella and Caballeronia positive and negative treatments. Bug performance was measured based on lifetime survival analysis, development time, and weight. Lifetime reproductive success was measured based on clutch size and egg hatch rate of adult female L. zonatus with and without Lariskella. In a second experiment, we evaluated egg mortality in reproductive crosses with and without Lariskella (in all four possible combinations) to evaluate the possibility that Lariskella caused cytoplasmic incompatibility. Finally, we sampled bugs from three L. zonatus populations in USA on pomegranates: two populations in California (Bakersfield and Fresno) and one from Tucson, Arizona to determine Lariskella infection frequency in the field.
This README file was generated on 2025-01-25 by Edwin F. Umanzor
##################### General information ###########################
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Title of Dataset: The facultative intracellular symbiont Lariskella is neutral for lifetime fitness and spreads through cytoplasmic incompatibility in the leaffooted bug, Leptoglossus zonatus
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Author Information
A. Principal Investigator Contact Information
Name: Martha S. Hunter
Institution: The University of Arizona
Address: Tucson, AZ USA
Email: mhunter@ag.arizona.eduB. Lead Author Contact Information
Name: Edwin F. Umanzor
Institution: The University of Arizona
Address: Tucson, AZ USA
Email: Edwinnzor@arizona.edu -
Date of data collection: 2018-2024
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Geographic location of data collection: Bakersfield (CA), Fresno (CA), Tucson (AZ)
##################### Data & File Overview ###########################
- File List:
A) Lariskella_development_titer.csv
B) Lariskella_reproductive_tissue_titer.csv
C) LxB_mortality_long.csv
D) LxB_development_long.csv
E) LxB_adult_weight_excluded_neg_PCR.csv
F) zonatus_egg_data_updated.csv
G) zonatus_CI_eggcounts_C2.csv
H) Lariskella_survey_2018-2019.csv
I) Temporal_survey_lariskella_screen.csv
##################### Data specific information ###########################
A) Lariskella_development_titer.csv
- Number of variables: 6
- Number of cases/rows: 26
- Variable List:
- stage: bug developmental stage
- sample: sample identifier
- volume(ul): total DNA extraction volume
- copy/2ul: copy number per 2ul used in qPCR
- copy/ul: copy number per 1ul
- copynum: total copy number based on total extraction volume
- Missing data codes: None
- Specialized formats or other abbreviations used: None
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B) Lariskella_reproductive_tissue_titer.csv
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Number of variables: 8
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Number of cases/rows: 17
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Variable List:
*tissue: reproductive tissye sampled
*sample: sample identifier
*concentration(ng/ul): DNA concentration
*volume(ul): total DNA extraction volume
*totalDNA(ng): Total amount of DNA extracted
*copy/4ng: copy number per 4ng of DNA
*copy/ng: copy number per 1ng of DNA
*copynum: total copy number based on total DNA extracted -
Missing data codes: None
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Specialized formats or other abbreviations used: None
#########################################################################
C) LxB_mortality_long.csv
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Number of variables: 8
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Number of cases/rows: 203
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Variable List:
*start: observation start date
*ddays: date of bug death or end of observation
*box: bug rearing box identifier
*id: bug identifier
*treatment: bug symbiont infection status: L-(Lariskella negative), L+(Lariskella positive), B-(Caballeronia negative), B+(Caballeronia positive)
*dayss: number of days survived or total observation time
*stage: bug development stage at time of death or end of observation
*status: 1= bug observed until it died, 0= observation interrupted -
Missing data codes: None
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Specialized formats or other abbreviations used: None
#########################################################################
D) LxB_development_long.csv
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Number of variables: 8
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Number of cases/rows: 850
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Variable List:
*start: hatch date
*obsdate: date when the next developmental stage was observed in the rearing box
*id: bug rearing box identifier
*treatment: bug symbiont infection status: L-(lariskella negative), L+(lariskella positive), B-(Caballeronia negative), B+(Caballeronia positive)
*days: number of days from hatch to observed developmental stage
*inocdate: date when Caballeronia was fed to 2nd instar bugs
*pop: bug developmental stage
*count: total number of bugs of the specified developmental stage -
Missing data codes: NA = not available, "count" variable has missing cells and kept empty to avoid any potential conflicts with R code. Number entered represents all bugs of the specific developmental stage, and empty cells = number entered - 1
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Specialized formats or other abbreviations used: None
#########################################################################
E) LxB_adult_weight_excluded_neg_PCR.csv
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Number of variables: 5
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Number of cases/rows: 86
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Variable List:
*box: bug rearing box identifier
*treatment: bug symbiont infection status: L-(lariskella negative), L+(lariskella positive), B-(Caballeronia negative), B+(Caballeronia positive)
*sex: bug sex (M=male, F=female)
*id: bug identifier
*weight(g): bug weight in grams -
Missing data codes: None
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Specialized formats or other abbreviations used: None
#########################################################################
F) zonatus_egg_data_updated.csv
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Number of variables: 16
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Number of cases/rows: 262
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Variable List:
*pairdate: cross start date
*ovidate: oviposition date
*p1: rearing box identifier of 1st individual in the cross
*p1sex: sex of first individual in a cross
*p1id: bug identifier of first individual in the cross
*p2: rearing box identifier of 2nd individual in the cross
*p2sex: sex of second individual in a cross
*p2id: bug identifier of second individual in the cross
*treatment: bug symbiont infection status: L-(lariskella negative), L+(lariskella positive), B-(Caballeronia negative), B+(Caballeronia positive)
*cross: both individuals in the cross
*hdate: egg hatch date
*days: number of days from pairing to hatching
*teggs: total number of eggs in clutch
*heggs: total number of eggs that hatched
*reggs: total number of eggs that failed to hatch
*dde: total number of nymphs that died during hatching -
Missing data codes: NA = not available
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Specialized formats or other abbreviations used: None
#########################################################################
G) zonatus_CI_eggcounts_C2.csv
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Number of variables: 11
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Number of cases/rows: 46
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Variable List:
*odate: oviposition date
*cross: infection status of both individual in the cross
*tegg: total number of eggs in clutch
*regg: total number of eggs with a normal chorion and a fully formed embryo inside
*hatch: percentage of "tegg"s that are classified as "regg"s
*fegg: total number of c1 and c2 eggs
*dde: total number of nymphs that died during hatching
*c1: total number of eggs that failed to hatch but have a normal colored chorion and a fully formed embryo inside
*c2: total number of eggs that failed to hatch but have a transluscent chorion and lacks a fully formed embryo inside
*id: rearing box identifier
*hdate: egg hatch date -
Missing data codes: None
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Specialized formats or other abbreviations used: L-(lariskella negative), L+(lariskella positive), B-(Caballeronia negative), B+(Caballeronia positive), M=male, F=female
#########################################################################
H) Lariskella_survey_2018-2019.csv
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Number of variables: 5
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Number of cases/rows: 248
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Variable List:
*extractionID: DNA extraction identifier
*bugid: bug identifier
*collectiondate: bug collection date
*region: bug collection region
*results: Lariskella PCR results (pos=positive, neg=negative) -
Missing data codes: None
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Specialized formats or other abbreviations used: None
#########################################################################
I) Temporal_survey_lariskella_screen.csv
- Number of variables: 2
- Number of cases/rows: 148
- Variable List:
- Date: bug collection date
- infection: Lariskella PCR results (pos=positive, neg=negative)
- Missing data codes: None
- Specialized formats or other abbreviations used: None