Data from: Vaginal bacteria-derived extracellular vesicles diffuse through human cervicovaginal mucus to enable microbe-host signaling
Data files
Dec 09, 2025 version files 363.96 KB
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bEV_diffusion_host_response_bEV_Characterization_data_clean.csv
1.70 KB
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bEV_diffusion_host_response_bEV_Characterization_removed_outliers.csv
957 B
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bEV_diffusion_host_response_bEV_Cytokine_data_clean.csv
9.31 KB
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bEV_diffusion_host_response_bEV_Cytokine_removed_outliers.csv
5.63 KB
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bEV_diffusion_host_response_bEV_Histogram_data_clean.csv
52.53 KB
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bEV_diffusion_host_response_bEV_Histogram_removed_outliers.csv
49.31 KB
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bEV_diffusion_host_response_bEV_uptake_data_clean.csv
5.08 KB
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bEV_diffusion_host_response_bEV_uptake_removed_outliers.csv
2.60 KB
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bEV_diffusion_host_response_bEV_viability_data_clean.csv
7.47 KB
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bEV_diffusion_host_response_bEV_viability_removed_outliers.csv
4.82 KB
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bEV_diffusion_host_response_CVM_1sMSD.csv
219.03 KB
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bEV_diffusion_host_response_CVM_SampleInfo.csv
930 B
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README.md
4.60 KB
Abstract
The composition of the vaginal microenvironment has significant implications for gynecologic and obstetric outcomes. Where a Lactobacillus-dominated microenvironment is considered optimal, a polymicrobial environment is associated with increased risk for female reproductive diseases. Recent work examined bacteria-derived extracellular vesicles (bEVs) as an important mode of microbe-host communication that may influence reproductive outcomes. However, in order to communicate with female reproductive tissues, bEVs must penetrate the protective cervicovaginal mucus barrier. We demonstrate increased diffusion of bEVs compared to whole bacteria. Additionally, we evaluate the uptake of bEVs by, and the resulting effects on, human vaginal epithelial, endometrial, and placental cells, highlighting potential mechanisms of action by which vaginal dysbiosis contributes to gynecologic and obstetric diseases. Taken together, our work demonstrates the ability of bEVs to mediate female reproductive outcomes and highlights their potential as therapeutic modalities for treating dysbiosis and dysbiosis-associated diseases in the female reproductive tract.
We have submitted our raw data, including bacterial extracellular vesicle (bEV) size distribution histograms (bEV_diffusion_host_response_bEV_Histogram_data_clean.csv), additional characterization (bEV_diffusion_host_response_bEV_Characterization_data_clean.csv), cervicovaginal mucus (CVM) sample information (bEV_diffusion_host_response_CVM_SampleInfo.csv), 1-second mean squared displacements (MSD) per particle (bEV_diffusion_host_response_CVM_1sMSD.csv), cell viability (bEV_diffusion_host_response_bEV_viability_data_clean.csv), particle uptake (bEV_diffusion_host_response_bEV_uptake_data_clean.csv), and cytokine response (bEV_diffusion_host_response_bEV_Cytokine_data_clean.csv).
Removed outliers can be found in the following associated CSV files:
- bEV_diffusion_host_response_bEV_Histogram_removed_outliers.csv
- bEV_diffusion_host_response_bEV_Characterization_removed_outliers.csv
- bEV_diffusion_host_response_bEV_uptake_removed_outliers.csv
- bEV_diffusion_host_response_bEV_viability_removed_outliers.csv
- bEV_diffusion_host_response_bEV_Cytokine_removed_outliers.csv
Descriptions
General Acronyms
- LC: Lactobacillus crispatus
- LI: Lactobacillus iners
- GV: Gardnerella vaginalis
- MM: Mobiluncus mulieris
- WB: whole bacteria
- bEV: bacterial extracellular vesicle
Cell Line Information
- VK2/E6E7: vaginal epithelium, ATCC CRL-2616
- Ishikawa: endometrium, Millipore Sigma 99040201
- BeWo-b30: placental trophoblast, ATCC CCL-98
Value Denotation
- GRUBB: Value removed as an outlier based on Grubbs’ test
- NA: No applicable data
- In removed_outlier.csv files, NA may indicate that the original value was not an outlier and can be found in the corresponding
data_clean.csvfile
- In removed_outlier.csv files, NA may indicate that the original value was not an outlier and can be found in the corresponding
bEV_diffusion_host_response_bEV_Histogram_data_clean.csv
- Histogram of the distribution of bEV size
- First column: size in nm
- Consecutive columns: independent replicates
- First row: bEV species
- Second row: replicate number
bEV_diffusion_host_response_bEV_Histogram_removed_outliers.csv
- Same formatting as the clean file
- Lists replicates removed due to Grubbs’ outlier test
bEV_diffusion_host_response_bEV_Characterization_data_clean.csv
- Compiled bEV characteristics by species
- First column: bEV species
- Second column: measurement type and units
- Particle concentration (bEV/mL), normalized to initial culture volume
- Size (nm)
- ZP (mV), zeta potential
- Protein content (µg/1e8 bEVs)
- Consecutive columns: replicates R1, R2, …, R6
bEV_diffusion_host_response_bEV_Characterization_removed_outliers.csv
- Same formatting as the clean file
- Lists replicates removed via Grubbs’ test
bEV_diffusion_host_response_CVM_SampleInfo.csv
- Lists the average 1-second MSD for all particle types (bEV and WB)
- Lists pH per CVM sample
- First column: sample number (as in publication)
- First row: species name and WB/bEV classification
- Last column: pH per sample
bEV_diffusion_host_response_CVM_1sMSD.csv
- Lists the 1-second MSD for each individual particle tracked
- First row: sample number
- Second row: species name
- Third row: bEV vs WB
- Subsequent rows: 1-second MSD for every analyzed particle
bEV_diffusion_host_response_bEV_uptake_data_clean.csv
- Percentage of internalized bEVs after a dosage of 5000 bEV/cell
- First column: cell line
- Second column: bEV species
- Third column: timepoints (h)
- Consecutive columns: replicates
bEV_diffusion_host_response_bEV_uptake_removed_outliers.csv
- Same formatting as the clean file
- Lists replicates removed via Grubbs’ test
bEV_diffusion_host_response_bEV_viability_data_clean.csv
- First column: cell line
- Second column: bEV species
- Third column: treatment concentration
- Consecutive columns: replicates
bEV_diffusion_host_response_bEV_viability_removed_outliers.csv
- Same formatting as the clean file
- Lists replicates removed via Grubbs’ test
bEV_diffusion_host_response_bEV_Cytokine_data_clean.csv
- First column: cell line
- Second column: cytokine measured
- Third column: treatment
- Consecutive columns: replicates
bEV_diffusion_host_response_bEV_Cytokine_removed_outliers.csv
- Same formatting as the clean file
- Lists replicates removed via Grubbs’ test