Low osmotic pressure induces calcium response states
Data files
Sep 03, 2025 version files 104.26 MB
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Fig_2_silence.avi
5.55 MB
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Fig_2_spontaneity.avi
11.12 MB
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Fig_2_wave.avi
9.22 MB
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Fig_2_wave2.avi
11.12 MB
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Fig_3_HUVEC.avi
2.64 MB
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Fig_3_MDCK.avi
2.18 MB
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Fig_3_RPTEC.avi
3.39 MB
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Fig_4-A_18A_GA.avi
33.36 MB
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Fig_4-A_Ca_free.avi
8.66 MB
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Fig_4-A_Gap27.avi
7.77 MB
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Fig_4-A_JTV_519.avi
7.12 MB
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Fig_4-C_right_RyR2_siRNA.avi
2.15 MB
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README.md
1.79 KB
Abstract
Osmotic pressure is critical for maintaining cellular homeostasis, but the mechanisms underlying osmotic responses remain incompletely understood. We show that acute osmotic changes regulate intracellular calcium concentration in three distinct modes. In addition, low osmotic pressure induces calcium flux from the endoplasmic reticulum through ryanodine receptors and propagates calcium signals to neighboring cells through gap junctions. Here, we represented raw data of calcium response modes under different conditions.
Dataset DOI: 10.5061/dryad.djh9w0wbg
Description of the data and file structure
This dataset originates from a study investigating how acute osmotic pressure changes influence intracellular calcium signaling in mammalian cells. The research identifies three distinct calcium response patterns triggered by osmotic shifts and characterizes a unique, wave-like signaling mode that enables coordinated responses across neighboring cells. This signaling mode is proposed to contribute to the early stages of osmotic stress-related organ injury.
Files and variables
File: Fig_2_wave.avi,File: Fig_2_silence.avi,Fig_2_spontaneity.avi,Fig_2_wave2.avi
Description: Live cell calcium videos. Silence mode - response to hypertonic solution: No calcium activation is observed after the stimulation with a hypertonic solution. Spontaneous activation mode: Cells exhibit an auto-activation response upon adding an isotonic solution, displaying a sparking-like activation pattern. Wave activation mode: Cell activation begins in one cell, spreads to neighboring cells, and shows activation spreading as a wave. The scale bar is 200 μm.
Files and variables
Files: Fig_3_HUVEC.avi, Fig_3_MDCK.avi, Fig_3_RPTEC.avi
Description: Live cell calcium video. RPTEC, MDCK cells, and HUVEC： calcium spontaneous activation mode in these cells. The scale bar is 200 μm.
Files: Fig_4-C_right_RyR2_siRNA.avi,Fig_4-A_Ca_free.avi,Fig_4-A_Gap27.avi,Fig_4-A_JTV_519.avi,Fig_4-A_18A_GA.avi
Description: Live cell calcium video. Blocking of RyR2 and gap junction with SiRNA, JTV-519, 18α-glycyrrhetinic acid (18A-GA), and Gap 27 inhibited wave activation mode. The scale bar is 200μm.