Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats
Data files
Mar 03, 2026 version files 71.35 KB
-
for_Dryad_raw_final_010326.xlsx
65.33 KB
-
README.md
6.02 KB
Abstract
Dataset DOI: 10.5061/dryad.f4qrfj78s
Description of the data and file structure
README – Dataset description
Dataset title
Comparison of hydrogen administration methods in the treatment of radiation-induced heart disease in rats
Dataset DOI: 10.5061/dryad.f4qrfj78s
1. Study overview
This dataset contains individual-level experimental data from a controlled animal study investigating the effects of different hydrogen administration methods on radiation-induced heart injury in rats.
All measurements are provided at the level of individual animals. The dataset allows independent reanalysis and full reconstruction of statistical results reported in the manuscript.
2. Experimental design
Animals were randomly assigned to four experimental groups:
- Control (non-irradiated)
- IR (irradiated)
- HRW (irradiated + hydrogen-rich water)
- HRA (irradiated + hydrogen-rich air)
Measurements were performed at two timepoints:
- 2 days post-irradiation (2d)
- 9 days post-irradiation (9d)
3. File description
File: for_Dryad_raw_final_010326.xlsx
The file contains one worksheet named:
Raw data – original laboratory worksheet name (no experimental significance).
Each row represents one measurement of a single parameter obtained from one animal at a specific timepoint. The dataset is organized in long-format (tidy data structure).
4. Variables
| Variable | Description | Possible values / Units |
|---|---|---|
| animal_id | Unique identifier for each animal | e.g., C1, IR3, HRW5 |
| group | Experimental group | Control, IR, HRW, HRA |
| timepoint | Time of measurement | 2d, 9d |
| measurement_type | Type of measurement | biochemistry, oxidative_stress, gene_expression |
| parameter | Measured biological parameter | see Section 5 |
| value | Measured numeric value | Units depend on parameter |
Missing values: Empty cells indicate missing measurements. No artificial numeric placeholders were used.
5. Parameter definitions and units
5.1 Biochemical parameters
| Parameter | Description | Units |
|---|---|---|
| ALB | Albumin | g/L |
| CRE | Creatinine | µmol/L |
| GLU | Glucose | mmol/L |
| ALT | Alanine aminotransferase | µkat/L |
| TCHO | Total cholesterol | mmol/L |
| UA | Uric acid | µmol/L |
5.2 Enzymatic and oxidative stress assays
For the following parameters, the dataset contains raw spectrophotometric absorbance values measured for individual samples. Final enzyme activities reported in the manuscript were calculated from these absorbance values according to the manufacturer's assay protocols.
| Parameter | Description | Units in dataset |
|---|---|---|
| lactate dehydrogenase-1st read | LDH assay, first technical measurement | absorbance (optical density, OD) |
| lactate dehydrogenase-2nd read | LDH assay, second technical measurement | absorbance (optical density, OD) |
| catalase | Catalase assay (CAT) | absorbance (OD) |
| glutathione peroxidase-1st read | GPx assay, first technical measurement | absorbance (OD) |
| glutathione peroxidase-2nd read | GPx assay, second technical measurement | absorbance (OD) |
| malondialdehyde | MDA assay (lipid peroxidation marker) | absorbance (OD) |
| superoxide | Superoxide chemiluminescence assay | cpm/mg |
Note: Conversion of absorbance values to enzyme activity units (e.g., U/mL, nmol/min/mL) was performed during statistical analysis using manufacturer-provided formulas and calibration factors.
5.3 Western blot protein expression
Western blot data represent raw densitometric band intensity values obtained from image analysis software (ImageJ).
The dataset includes:
- raw intensity values for target proteins
- raw intensity values for the housekeeping protein (GAPDH)
No normalization was applied within the dataset. Normalized protein expression values (target protein relative to GAPDH) reported in the manuscript were calculated during statistical analysis.
Units in dataset: arbitrary densitometric units (raw intensity values).
| Parameter | Description |
|---|---|
| IL-6 | Interleukin-6 protein expression |
| TNF-a | Tumor necrosis factor alpha protein expression |
| Nrf2 | Nuclear factor erythroid 2–related factor 2 protein expression |
| Keap1 | Kelch-like ECH-associated protein 1 protein expression |
| GAPDH | Housekeeping protein used for normalization |
6. Software
The dataset is provided as a Microsoft Excel (.xlsx) file and can be opened using:
- Microsoft Excel (2019 or later)
- LibreOffice Calc (7.0 or later)
- Google Sheets
Statistical analyses were performed using: GraphPad Prism version 9 (GraphPad Software, USA)
No custom scripts were used.