Effects of X-ray irradiation and housing conditions on mitochondria in Peromyscus maniculatus
Data files
Apr 23, 2025 version files 72.17 KB
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README.md
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Rudolf_et_al._2023_X-rays_Peromyscus_-_Data.xlsx
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Abstract
Challenging environmental conditions, such as exposure to ionizing radiation, can induce oxidative stress within mitochondria. However, organisms show variation in their capacity to cope with cellular stress. This study aimed to identify how housing conditions influence mitochondrial stress responses under exposure to radiation. We used North American deer mice (Peromyscus maniculatus), kept in standard rodent boxes or large semi-natural enclosures that allowed for natural levels of physical activity and social structures under ambient atmospheric conditions. Animals from each housing condition were exposed to 2000 mSv (2 Gy) of ionizing X-ray radiation, a dose comparable to long-term spaceflight. Animals were euthanized for analysis prior to irradiation (baseline), within four days post-irradiation, or one-month following irradiation. Relative mass of the liver was lower than the baseline for irradiated animals maintained in the semi-natural enclosures. State 4 and coupled and uncoupled state 3 respiratory capacity of liver and skeletal muscle mitochondria were lower four days after the exposure. Yet, a month after exposure these variables were comparable to baseline in muscle but remained lower in the liver. The mitochondrial volume was lower after irradiation relative to baseline and was higher in animals maintained in standard rodent boxes, in both tissues. Oxidative damage to lipids in liver was higher in animals kept in boxes, whereas in muscle it was higher in animals kept in the semi-natural enclosures, and oxidative damage to proteins did not change. These results suggest that a heterogenous environment with greater potential for activity may counter the effects of ionizing radiation, although this effect appears to be modest.
https://doi.org/10.5061/dryad.fqz612k05
The data are presented in a single Excel file.
Description of the data and file structure
Within the Excel file, all tabs include:
- each animal's unique 'animal tag' number
- timing of 'irradiation group' which occurred at baseline (before irradiation), 1-4 'days after' irradiation, or a 'month after' irradiation
- 'housing group' - animals were housed in a 'box' or a semi-natural 'enclosure'
- 'sex' - male or female
- 'box/enclosure nr' - where animals were housed (different letters designate different enclosures, different number designate different boxes - each number or letter is arbitrary)
Tab 'Body_Mass'
- Body mass for each week at Auburn University is presented ('Body mass week 1', 'Body mass week 2', etc.), as well as the average of all data, 'Body Mass Average'.
Tab "Liver_Respiraiton"
- 'Body Mass at dissection'
- 'Liver Mass'
- 'Date dissection' is presented as DD.MM.YEAR
- 'Chamber Oroboros Liver' - the Oroboros has two chambers, this identifies which was used (A is the left chamber, B is the right).
- Sample Code - used for labelling, comparable to the animal tag nr
- 'Isolated mitochondria flux' oxygen utilization following injections of 5 mM pyruvate, 5 mM malate, and 10 mM glutamate [O2 pmol/s*ml] isolated mitochondria
- 'Routine flux' - oxygen utilization following injections of 5 mM pyruvate, 5 mM malate, and 10 mM glutamate [O2 pmol/s*ml]
- 'Oxphos CI flux' - Routine flux followed by 1 mM ADP [O2 pmol/s*ml]
- 'Oxphos flux' - Oxphos CI flux followed by 10 mM succinate [O2 pmol/s*ml]
- 'Leak flux' - Oxphos flux followed by 1.25 µM oligomycin [O2 pmol/s*ml]
- 'ETS flux' - Leak flux followed by injections of 0.5, 0.25, 0.25 µM FCCP [O2 pmol/s*ml]
- 'ETS CII flux' - ETS flux followed by 1 µM rotenone [O2 pmol/s*ml]
- 'Rox flux' - ETS flux followed by 1.25 µM antimycin A [O2 pmol/s*ml]
- 'Routine' - Routine flux minus Rox flux (non-mitochondrial respiration) [O2 pmol/s*ml]
- 'Oxphos CI' - Oxphos CI flux minus Rox flux [O2 pmol/s*ml]
- 'Oxphos CII' - Oxphos CII flux minus Rox flux [O2 pmol/s*ml]
- 'Oxphos' - Oxphos flux minus Rox flux [O2 pmol/s*ml]
- 'Leak' - Oxphos CI Leak flux minus Rox flux [O2 pmol/s*ml]
- 'ETS' - Oxphos CI ETS flux minus Rox flux [O2 pmol/s*ml]
- 'ETS CI' - Oxphos CI ETS CI flux minus Rox flux [O2 pmol/s*ml]
- 'ETS CII' - Oxphos CI ETS CII flux minus Rox flux [O2 pmol/s*ml]
- 'RCR Oxphos/Leak' - Oxphos divided by Leak
- 'RCR ETS/Leak' - ETS divided by Leak
- 'Remarks' - describes issue with measurements
Tab "Muscle_Respiraiton"
- similar to Liver_Respiration except the leg 'Muscle Mass' given rather than liver
Tab "Oxidative Stress"
- MDA uM/ml Liver - Concentration of malondialdehyde (MDA) measured with TBARS method in liver
- Protein mg/ml Liver - Concentration of protein in liver sample
- MDA uM/mg Liver - Concentration of malondialdehyde (MDA) per mg protein in liver
- MDA uM/ml Muscle - Concentration of malondialdehyde (MDA) measured with TBARS method in muscle
- Protein mg/ml Muscle - Concentration of protein in muscle sample
- MDA uM/mg Muscle - Concentration of malondialdehyde (MDA) per mg protein in muscle
- ProteinCarbonyls nmol/ml Liver - Concentration of protein carbonyls in liver
- Protein mg/ml Liver - Concentration of protein in liver sample
- ProteinCarbonyls nmol/ml/mg Liver - Concentration of protein carbonyls per mg protein in liver
In all cases, if data is not available, the cell has been left blank.
Sharing/Access information
Data is also available by request from Rudolf or Hood.
Data were collected from deer mice housed in boxes (3 groups) or large semi-natural enclosures (3 groups). Within each housing environment, data were collected from animals at baseline (before radiation exposure), 1-4 days after radiation exposure (days after), and one month after exposure. Liver and skeletal muscles were harvested from each deer mouse. For mitochondrial respiration measurements, mitochondria were isolated, and mitochondrial respiration was run on an Oroboros High-Resolution Respirometer. To quantity oxidative stress in each organ, TBARS and protein carbonyls were measured in each group. These data are presented as raw values in an Excel file. Note that there are multiple tabs in the file.
- Rudolf, Agata M; Rhodes, Emma M; Yamada, KayLene YH et al. (2025). Effects of X-ray irradiation and housing conditions on mitochondria in Peromyscus maniculatus. Life Sciences in Space Research. https://doi.org/10.1016/j.lssr.2025.02.002