Lactose operon sequences from: Spatial-scale evolutionary bias sheds light on the latitudinal diversity gradient
Data files
Oct 23, 2025 version files 68.61 KB
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README.md
998 B
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Sequence_Alignment.fas
67.61 KB
Abstract
We placed the ancestral strain lac-ancestor of Escherichia coli K-12 GM4792 in two types of plates (Blue-plate and White-plate) for experimental evolution, with 24 replicates in each group. Both types of plates contained liquid medium and semi-solid medium sections. After 13 days of evolution, we randomly selected one single clone from both the liquid and semi-solid media of the 24 Blue plates for sequencing, and also sequenced one single clone from the semi-solid medium of the 24 White-plates, for a total of 72 single clones sequenced. We sequenced a gene fragment in the lactose operon region. The data show the sequence alignment of these 72 single clones. Additionally, we also included the gene sequences of the lac-ancestor and Escherichia coli K-12 MG1655.
Dataset DOI: 10.5061/dryad.ghx3ffc2s
Description of the data and file structure
This dataset consists of a sequence alignment file (Sequence_Alignment.fas in fastq format) of an 873bp segment of the lactose operon region gene. These sequences are derived from 72 single clones from the ancestral strain lac-ancestor (Escherichia coli K-12 GM4792) and Escherichia coli K-12 MG1655. These 72 single clones come from two types of plates (Blue-plate and Whsingle cloneshich were used for the spatial-scale evolutionary bias experimental evolution. Through this sequence alignment, a 212bp sequence deletion can be observed, which characterizes Escherichia coli K-12 GM4792 used in our study. Additionally, mutation sites associated with lactose utilization evolution can be seen in 24 single clones derived from the Blue-plate.