Dataset DOI: 10.5061/dryad.gmsbcc30t

Description of the data and file structure

Data wing size: This file contains the value of wing size of both Drosophila species reared in four cactus of the Trichocereus genus for each selection strain.

Data wing shape: This file contains the value of each realtive warp for each Drosophila species raised in four cactus of the Trichocereus species for each selection strain. The name of the photo taken of the wing is also incorporated.

Data of flutuating asymmetry: This file contains the wing size value for right and left wing of both Drosophila species raised in four cactus of the Trichocereus genus for each selection strain. 

Files and variables

File: Data_Fluctuating_asymmetry.xlsx

Description: Developmental instability was estimated through the Fluctuating Asymmetry (FA) of centroid size, using data of both wings of seven randomly chosen males per treatment (i.e., each combination of strain and cactus). Landmark positioning was done twice for each wing to estimate measurement error. This dataset includes the centroid size of each Drosophila species (both, the left and right wings) reared in each cactus hosts for each artificial selection strain. This dataset was used to perform the Palmer ANOVA to calculate FA.

Variables: The explanatory variables are cactus, strain and species and the response variable is the centroid and the units are pixels.

File: Data_Wing_Shape.xlsx

Description:  We photographed between 7 and 15 pairs of wings of males per combination of strain and cactus for each Drosophila species (276 pairs in total). Then, we placed 10 landmarks at the junctions of the most representative veins or the junction of the veins and wing margin in each image using tpsDig. The Procrustes technique was utilized to quantify wing morphology, employing the Morpho package. All wings were superimposed to minimize differences relative to size, position and orientation. This procedure generated 20 new Procrustes coordinates and eliminated four degrees of freedom, resulting in 16 shape-space dimensions. Shape variables generated afterwards, known as partial warps, indicate the partial contributions of hierarchically scaled vectors spanning a linear shape-space. Subsequently, principal components analysis of the partial warps scores matrix was conducted to obtain 16 new shape variables called relative warps (RWs). Hence, the dataset includes 16 relative warps for both Drosophila species reared in each cactus host for each artificial selection strain.

Variables: The explanatory factors are cactus, strain, and species and the response variables are the relative warps. It does not have unities.

File: Data_Wing_size.xlsx

Description: We photographed between 7 and 15 pairs of wings of males per combination of strain and cactus for each Drosophila species (276 pairs in total). Then, we placed 10 landmarks at the junctions of the most representative veins or the junction of the veins and wing margin in each image using tpsDig. Centroid size was calculated as the square root of the sum of the squared distances from each landmark to the centroid (geometric center) of the configuration of landmarks. The dataset includes the centroid size of the wing for both Drosophila species reared in different cactus hosts for each ariticial selection strain.

Variables: The explanatory factors are wing,cactus, strain, and species; the response variable is the centroid and the units are pixels.

Variables:

• Cactus (host cactus studied): T. terscheckii, T. schickendantzii,T. atacamensis, and T. tarijensis

• Strain (Strains of experimental flies): Control, S1 (: Selection regimen 1 (S1), corresponded to a balanced mix of 50/50% standard and chlorenchyma medium), S2 (Selection regimen 2 (S2), a more challenging rearing media, was composed of 25% standard medium and 75% chlorenchyma powder)

• Species: D. koepferae and D. buzzatii

• Wing: Left or Right

Datasets do not include missing values.