Dataset DOI: 10.5061/dryad.hx3ffbgrt

Description of the data and file structure

Dataset Overview

This dataset contains the processed data used to test the hypothesis that the mitochondrial enzyme Arginase 2 (ARG2) contributes to cardiac aging. Specifically, the study demonstrates that ARG2 is not expressed in cardiomyocytes from aged mice and human cardiac tissue samples, but is upregulated in non-myocyte cell populations of the aging heart, including macrophages, fibroblasts, and endothelial cells.

The dataset covers a range of measurements performed in both young and old wild-type (wt) mice and Arg2 knockout (Arg2^-/-) mice. The assessments include markers of age-associated cardiac inflammation, cardiomyocyte apoptosis, interstitial and perivascular fibrosis, endothelial-to-mesenchymal transition (EndMT), and susceptibility to ischemic injury.

Data were generated using conventional and standardized molecular biology techniques such as real-time quantitative PCR (RT-qPCR), Western blotting, and immunofluorescence microscopy followed by quantitative image analysis. All individual data points underlying each graph or figure panel are reported in the associated data files.

Data Organization

The data are organized in files named according to the figure they correspond to in the associated manuscript. For example:

Figure_1-source_data_1.zip contains data relevant to Figure 1.

Files and variables

Figure1-source_data_1 (GraphPad Prism file)

This file contains experimental data underlying the analyses related to cardiac fibrosis and aging in wild type (Wt) and Arg2 knockout (KO; Arg2^-/-) mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

Both male and female animals are included where indicated.

Contents of the data tables:

-          Figure 1A: Expression levels of ARG2 in young and old male and female Wt mice

-          Figure 1C: Percentage of fibrotic area in Wt vs. Arg2^-/- (young and old)

-          Figure 1D: Hydroxyproline content of left ventricular (LV) tissue in Wt vs. Arg2^-/-^ ^(young and old)

-          Figure 1F: PDGFRα positive signal across groups

-          Figure 1H: Number of P16-positive nuclei across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_1_–_figure_supplement_1-source_data1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to Arg2 and Arg1 expression in Wt and Arg2 knockout (KO; Arg2^-/-) mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

-          6mo, 18mo and 27mo = 6-, 18- and 27-months old animals

Both male and female animals are included where indicated.

Contents of the data tables:

-          Figure 1 – figure supplement 1-source data1_A: Expression levels of Arg1 in young and old female Wt and Arg2-/- mice

-          Figure 1 – figure supplement 1-source data1_B: Expression levels of Arg1 in young and old male Wt mice

-          Figure 1 – figure supplement 1-source data1_C: Expression levels of Arg2 in 6-, 18- and 27-months old Wt mice

-          Figure 1 – figure supplement 1-source data1_D: Expression levels of Arg1 in 6-, 18- and 27-months old Wt mice

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_1_–_figure_supplement_2-source_data1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to collagen genes (Col1a1 and Col3a1) expression in Wt and Arg2 knockout (KO; Arg2^-/-) mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

Contents of the data tables:

-          Figure 1 – figure supplement 1-source data2_A: Expression levels of Col1a1 in young and old female Wt and Arg2^-/- mice

-          Figure 1 – figure supplement 1-source data2_B: Expression levels of Col3a1 in young and old female Wt and Arg2^-/- mice

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_2-source_data_1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to cardiac inflammation and macrophage characterization (infiltrating Vs resident cells) in Wt and Arg2 knockout (KO; Arg2^-/-) young and old mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

Contents of the data tables:

-          Figure 2A: Expression levels of Adgre1 (gene name of the macrophage specific marker F4-80) in young and old female Wt and Arg2^-/- mice

-          Figure 2B: Expression levels of Mcp1 in Wt vs. Arg2^-/- (young and old females)

-          Figure 2C: Expression levels of Il1b in Wt vs. Arg2^-/- (young and old females)

-          Figure 2D: Expression levels of Tnfa in Wt vs. Arg2^-/- (young and old females)

-          Figure 2G: F4-80 positive cells across groups

-          Figure 2H: LYVE1 (resident marker) and F4-80 double positive cells across groups

-          Figure 2I: CCR2 (infiltrating marker) and F4-80 double positive cells across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_2_-_figure_supplement_1-source_data1.zip (TIFF image files)

This folder contains microscopy images used to validate antibodies for macrophage population characterization. These data are provided as underlying material for the antibody validation experiments and are not restricted to the images shown in the manuscript. F4-80 is used as mouse pan-macrophage marker. LYVE1 identifies resident macrophages. CCR2 identifies infiltrating macrophages.

-          LYVE1 / F4-80 colocalization images: Demonstrates LYVE1 as a marker for resident macrophages within the total F4-80⁺ macrophage population.

-          CCR2 / F4-80 colocalization images: Demonstrates CCR2 as a marker for infiltrating macrophages within the total F4-80⁺ population.

Negative control images: provided for both LYVE1/F4-80 and CCR2/F4-80 staining to confirm antibody specificity.

Figure_3-source_data_1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to cardiac apoptosis (TUNEL positive cells were quantified) in Wt and *Arg2 *knockout (KO; Arg2^-/-) young and old mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

-          Non-myoc = non myocytes cells

-          Myoc = cardiomyocytes

Contents of the data tables:

-          Figure 3B: TUNEL positive cells across groups

-          Figure 3D: TUNEL positive cardiomyocytes and non-myocytes cells in Wt and Arg2^-/- animals

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure4-source_data_1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to endothelial to mesenchymal transition (EndMT) in cardiac tissue during aging in Wt and Arg2 knockout (KO; Arg2^-/-) young and old mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = *Arg2^-/- ^*animals

-          Y = young animals

-          O = old animals

Contents of the data tables:

-          Table 4B: protein level of SNAIL (master regulator of EndMT) across groups

-          Table 4C: protein level of Vimentin (mesenchymal marker) across groups

-          Table 4D: protein level of CD31 (endothelial marker) across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure4-source_data_2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include SNAIL, Vimentin, and CD31, with normalization to the respective loading controls (Tubulin or Vinculin).

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure4-source_data_3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find sub-folders with following structure:

-          CD31 - raw membrane images for CD31 staining

-          SNAIL - raw membrane images for SNAIL staining

-          Tubulin - raw membrane images for Tubulin loading control

-          Vimentin - raw membrane images for Vimentin staining

-          Vinculin - raw membrane images for Vinculin loading control

These raw images provide the underlying data for the quantifications reported in the manuscript.

Figure_4_–figure_supplement_1-_source_data1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to heart weight to body weight of Wt young mice in comparison to old Wt and Arg2^-/- old mice. The file includes one data table where experimental groups are organized in separate columns.

Experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

The table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure5-source_data_1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related Arg2 expression levels in myocytes and non-myocytes cells isolated from Wt and Arg2^-/- mice. The file includes one data table where experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          Non-myoc = non myocytes cells

-          Myoc = cardiomyocytes

The table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure5-source_data_2.zip (PDF file)

This file contains uncropped western blot membranes shown in Figure 5C. The blots show the level of ARG2 in cardiomyocytes and fibroblasts isolated from mouse heart upon culturing at hypoxic conditions (1% Oxygen). The proteins analysed include ARG2 and the respective loading control Tubulin.

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          C = control

-          N = normoxia

-          H = hypoxia 1% oxygen

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          K = mouse kidney protein extract

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure5-source_data_3.zip (TIFF image files)

This folder contains the original TIFF image shown in the related figure. In the folder the user will find both raw membrane images for ARG2 and the related loading control Tubulin.

Figure6-source_data_1.zip (GraphPad Prism file)

This file contains experimental data underlying the analyses related to IL1β protein levels in cardiac tissue during aging in Wt and Arg2^-/- young and old mice. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Wt = wild type animals

-          KO = Arg2^-/- animals

-          O = old animals

Contents of the data tables:

-          Figure 6B: protein level of IL1β across groups

-          Figure 6D: IL1β positive signal across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure6-source_data_2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include IL1β and the respective loading control Tubulin.

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          Wt = wild type animals

-          Arg2^-/- = Arg2^-/- animals

-          Young = young mice

-          Old = old mice

-          Raw = RAW 264.7 cell line

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure6-source_data_3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find sub-folder with following structure:

-          IL1β - raw membrane images for IL1β

-          Tubulin - raw membrane images for Tubulin loading control

These raw images provide the underlying data for the quantifications reported in the manuscript.

Figure7-source_data1.zip (GraphPad Prism file)

This file contains experimental data underlying analyses of macrophage-cardiomyocyte crosstalk in vitro. The datasets include measurements of ARG2 and IL1β protein levels in spleen-derived macrophages isolated from Wt and Arg2^-/- mice, both young and old. In addition, the file includes data on cardiomyocyte responses to macrophage-conditioned media, evaluated by the percentage of TUNEL-positive cells as an indicator of apoptosis. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          Y = young animals

-          O = old animals

-          ILRa = IL receptor antagonist

Contents of the data tables:

-          Figure 7B: protein level of Arg2 across groups

-          Figure 7C: protein level of IL1β across groups

-          Figure 7D: protein level of IL1β across groups assessed by ELISA of conditioned media

-          Figure 7G: % of TUNEL positive cardiomyocytes cells

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure7-source_data2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include ARG2, IL1β and the respective loading control Tubulin.

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          MØ = macrophage cells

-          Wt = wild type animals

-          Arg2^-/- = Arg2^-/- animals

-          Y = young mice

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure7-source_data3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find sub-folder with following structure:

-          ARG2 - raw membrane images for ARG2

-          IL1β - raw membrane images for IL1β

-          Tubulin - raw membrane images for Tubulin loading control

These raw images provide the underlying data for the quantifications reported in the manuscript.

Figure7-_figure_supplement_1-source_data1.zip (GraphPad Prism file)

This file contains experimental data underlying analyses of RAW 264.7 mouse macrophage-cardiomyocyte crosstalk in vitro. The datasets include measurements of ARG2 and IL1β protein levels in RAW cells under LPS stimulation and Arg2 gene silencing. In addition, the file includes data on cardiomyocyte responses to macrophage-conditioned media, evaluated by the percentage of TUNEL-positive cells as an indicator of apoptosis. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          Lacz = control rAd expressing shRNA targeting Lacz

-          Arg2 = rAd expressing shRNA targeting Arg2

-          C = control condition

-          LPS = LPS stimulation

-          CM = conditioned media

Contents of the data tables:

-          Figure7-figure_supplement1-source_data_1B: protein level of ARG2 across groups

-          Figure7-figure_supplement1-source_data_1C: protein level of IL-1β across groups

-          Figure7-figure_supplement1-source_data_1E: : % of TUNEL positive cardiomyocytes cells

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure7-_figure_supplement_1-source_data2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include ARG2, IL1β and the respective loading control Tubulin.

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          Lacz = control rAd expressing shRNA targeting Lacz

-          Arg2 = rAd expressing shRNA targeting Arg2

-          LPS = LPS stimulation

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure7-_figure_supplement_1-source_data3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find sub-folder with following structure:

-          ARG2 - raw membrane images for ARG2

-          IL1β - raw membrane images for IL-1β

-          Tubulin - raw membrane images for Tubulin loading control

These raw images provide the underlying data for the quantifications reported in the manuscript

Figure_7_–figure_supplement_2-_source_data1.zip (GraphPad Prism file)

This dataset contains the experimental data underlying the analyses of ARG2 and NOS2 in the regulation of IL-1β production in macrophages. The data include both protein and mRNA expression analyses performed in human THP1 macrophage-like cells, and protein levels analysis performed using mouse bone-marrow-derived macrophages (BMDMs) under LPS stimulation. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          WT = wild type THP1 or BMDM cells

-          Arg2^-/- = Arg2 knockout THP1

-          NOS2^-/- = NOS2 knockout BMDMs

-          LPS = LPS stimulation

The table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_7_–figure_supplement_2-_source_data2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include NOS2, ARG2 and IL-1β with the respective loading controls (Tubulin or GAPDH).

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          THP1^wt = wild type THP1 cells

-          THP1^Arg2-/- = Arg2 knockout THP1 cells

-          MØ = mouse BMDM macrophage cells

-          MØ^wt = wild type BMDMs

-          MØ^NOS2-/- = NOS2^-/- BMDMs

-          LPS = LPS stimulation

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure_7_–figure_supplement_2-_source_data3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find 2 sub-folders with following structure:

1.      Figure 7 -figure supplement 2A - raw membrane images for ARG2, NOS2 and Tubulin

2.      Figure 7 -figure supplement 2D:

a.      ARG2 - raw membrane images for ARG2

b.      GAPDH - raw membrane images for GAPDH

c.      IL-1β - raw membrane images for IL-1β

d.      NOS2 - raw membrane images for NOS2

These raw images provide the underlying data for the quantifications reported in the manuscript

Figure8-source_data1.zip (GraphPad Prism file)

This file contains quantitative data underlying analyses of the crosstalk between splenic macrophages and cardiac fibroblasts and the cell-autonomous effects of ARG2 in cardiac fibroblasts. The data were collected from multiple in vitro experiments evaluating fibroblast responses to macrophage-conditioned media and to Arg2 modulation. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Labels to interpret experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          O = old animals

-          CM = conditioned media

-          C = control

-          LacZ = transfection with rAd-CMV-Control

-          Arg2+ = transfection with rAd-CMV-Arg2

-          TEMPO = mito-TEMPO used to dampen mitochondrial-derived ROS

-          Lacz-Sil = control rAd expressing shRNA targeting Lacz

-          Arg2-Sil = rAd expressing shRNA targeting Arg2

-          N = Normoxia

-          H = Hypoxia

Contents of the data tables:

-          Figure 8A: Hydroxyproline levels across groups

-          Figure 8B: expression levels of *Fib *across groups

-          Figure 8C: expression levels of Col3a across groups

-          Figure 8D: expression levels of Tgfb1 across groups

-          Figure 8E: Hydroxyproline levels across groups

-          Figure 8G: expression levels of Arg2 across groups

-          Figure 8H: protein levels of Vimentin across groups

-          Figure 8I: expression levels of Col3a across groups

-          Figure 8K: MitoSox signal detected in fibroblasts under the reported conditions

-          Figure 8L: expression levels of Col3a in fibroblasts under the reported conditions

-          Figure 8P: MitoSox signal detected in fibroblasts under the reported conditions

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure8-source_data2.zip (PDF file)

This file contains uncropped western blot membranes used for quantification of protein levels. The proteins analysed include Vimentin, ARG2 and HIF-1α with the respective loading controls (Tubulin or Gapdh).

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          CMV Lacz = transfection with rAd-CMV-Control

-          CMV Arg2 = transfection with rAd-CMV-Arg2

-          Hypoxia = culturing under hypoxic conditions (1% oxygen level)

-          M Kidney = mouse kidney protein extract used as positive control

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure8-source_data3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find 2 sub-folders with following structure:

1.      Figure 8F:

a.      ARG2 - raw membrane images for ARG2

b.      GAPDH - raw membrane images for GAPDH

c.      Vimentin - raw membrane images for Vimentin

2.      Figure 8M - raw membrane images for ARG2, Hif1α and Tubulin

These raw images provide the underlying data for the quantifications reported in the manuscript

Figure_8_–figure_supplement_1-source_data1.zip (GraphPad Prism file)

This file contains quantitative data underlying analyses of the crosstalk between splenic macrophages and cardiac fibroblasts and the cell-autonomous effects of ARG2 in cardiac fibroblasts. The data were collected from multiple in vitro experiments evaluating fibroblast responses to macrophage-conditioned media and to ARG2 modulation. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Labels to interpret experimental groups:

-          WT = wild type animals

-          KO = Arg2-/- animals

-          Y = young animals

-          O = old animals

Contents of the data tables:

-          Figure8-figure_supplement1-A: Hydroxyproline levels across groups

-          Figure8-figure_supplement1-B: expression levels of *Mmp2 *across groups

-          Figure8-figure_supplement1-C: expression levels of Mmp9 across groups

-          Figure8-figure_supplement1-D: expression levels of Col1a1 across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure9-source_data1.zip (GraphPad Prism file)

This file contains quantitative data underlying analyses of endothelial-to-mesenchymal transition (EndMT) in human umbilical vein endothelial cells (HUVECs) exposed to conditioned media (CM) from splenic macrophages of young and old mice, either Wt or Arg2^-/-. The experiments investigate how aging and Arg2 expression in macrophages regulate EndMT marker expression in endothelial cells. The file includes multiple data tables, each corresponding to a different experimental measurement. For each dataset, experimental groups are organized in separate columns.

Experimental groups:

-          WT = wild type animals

-          KO = *Arg2^-/- ^*animals

-          Y = young animals

-          O = old animals

-          C = control

-          IRA = cells treated with IL receptor antagonist

-          CM = conditioned media

Contents of the data tables:

-          Figure 9B: protein levels of ARG2 across groups

-          Figure 9C: protein levels of N-Cadherin across groups

-          Figure 9D: protein levels of Vimentin across groups

-          Figure 9E: protein levels of VE-Cadherin across groups

-          Figure 9G: protein levels of ARG2 across groups

-          Figure 9H: protein levels of N-Cadherin across groups

-          Figure 9I: protein levels of Vimentin across groups

-          Figure 9J: protein levels of VE-Cadherin across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure9-source_data2.zip (PDF file)

These files contain uncropped western blot membranes used for quantification of protein levels. The proteins analysed include ARG2, Vimentin, N-Cadherin and VE-Cadherin with the respective loading control Gapdh.

Each image includes:

-          The relevant experimental group or condition

-          The molecular weight marker

-          The full, uncropped membrane image

Experimental groups:

-          WT = wild type animals

-          Arg2^-/- = Arg2^-/- ^animals

-          Young = young animals

-          Old = old animals

-          MØ-CM = conditioned media collected from splenic macrophages

-          IRA = cells treated with IL receptor antagonist

Representative blot regions used for visualization in the associated publication are indicated with a red dotted outline.

Figure9-source_data3.zip (TIFF image files)

This folder contains the original TIFF image files used for quantification of protein expression shown in the related analysis. In the folder the user will find 2 sub-folders with following structure:

1.      Figure 9A:

a.      ARG2 - raw membrane images for ARG2

b.      GAPDH - raw membrane images for GAPDH

c.      Vimentin - raw membrane images for Vimentin

d.      N-CAD - raw membrane images for N-Cadherin

e.      V-CAD - raw membrane images for VE-Cadherin

2.      Figure 9F:

a.      ARG2 - raw membrane images for ARG2

b.      GAPDH - raw membrane images for GAPDH

c.      Vimentin - raw membrane images for Vimentin

d.      N-CAD - raw membrane images for N-Cadherin

e.      V-CAD - raw membrane images for VE-Cadherin

These raw images provide the underlying data for the quantifications reported in the manuscript

Figure10-source_data1.zip (GraphPad Prism file)

This file contains quantitative data underlying the analysis of cardiac functional recovery following global ischemia/reperfusion (I/R) injury in ex-vivo Langendorff-perfused hearts from aged female mice. The experiment evaluates the impact of Arg2 gene ablation on myocardial performance during reperfusion. The file includes multiple data tables, each corresponding to a different experimental measurement. For figure 10B each dataset column (e.g. Figure10B_DataSource1 – A:Y1 to A:Y5) shows the time course (in minutes) of the recovery from I/R injury of either WT or Arg2^-/- animals.

Experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          O = old animals

Contents of the data tables:

-          Table 10B_1: recovery of left ventricular developed pressure (LVDP) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Table 10B_2: recovery of left ventricular end-diastolic pressure (LVEDP) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Table 10B_3: recovery of maximal rate of contraction of LV (dP/dT max) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Table 10B_4: recovery of relaxation rate of LV (dP/dT min) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Table 10D: Infarct size across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.

Figure_10_–figure_supplement_1-_source_data_1.zip (GraphPad Prism file)

This file contains quantitative data underlying the analysis of myocardial injury following short-duration global ischemia/reperfusion (I/R) in ex vivo Langendorff-perfused hearts from female mice. The experiment evaluates the extent of infarction in Wt and Arg2^-/- animals after a mild I/R protocol.

Experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          O = old animals

Contents of the data tables:

-          Figure10-figure_supplement1B: Infarct size across groups

Figure_10_–figure_supplement_2-_source_data_1.zip (GraphPad Prism file)

This file contains quantitative data underlying the analysis of cardiac functional recovery following global ischemia/reperfusion (I/R) injury in ex-vivo Langendorff-perfused hearts from aged male mice. The experiment evaluates the impact of Arg2 gene ablation on myocardial performance during reperfusion. The file includes multiple data tables, each corresponding to a different experimental measurement. For Figure10-figure_supplement2-B each dataset column (e.g. Figure10-figure_supplement2-B_1 – A:Y1 to A:Y5) shows the time course (in minutes) of the recovery from I/R injury of either WT or Arg2^-/- animals.

Experimental groups:

-          WT = wild type animals

-          KO = Arg2^-/- animals

-          O = old animals

Contents of the data tables:

-          Figure10-figure_supplement2-B_1: recovery of left ventricular developed pressure (LVDP) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Figure10-figure_supplement2-B_2: recovery of left ventricular end-diastolic pressure (LVEDP) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Figure10-figure_supplement2-B_3: recovery of maximal rate of contraction of LV (dP/dT max) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Figure10-figure_supplement2-B_4: recovery of relaxation rate of LV (dP/dT min) after ischemia / reperfusion in ex-vivo Langendorf experiment across groups

-          Figure10-figure_supplement2-D: Infarct size across groups

Each table is accompanied by a simple graph generated in GraphPad Prism to visualize the data distribution.