Data from: The effect of inactivated bacteria on the redox status of larvae of the wax moth Galleria mellonella
Data files
Aug 20, 2025 version files 108.82 KB
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README.md
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Yana_L._Vorontsova_Figure_1_The_survival_of_wax_moth.xlsx
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Yana_L._Vorontsova_Figure_2a_The_total_hemocyte_count.xlsx
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Yana_L._Vorontsova_Figure_2b_Activity_of_phenoloxidase.xlsx
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Yana_L._Vorontsova_Figure_3a_Generation_of_ROS.xlsx
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Yana_L._Vorontsova_Figure_3b_Total_antioxidant_activity_in_hemolymph.xlsx
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Yana_L._Vorontsova_Figure_3c_Generation_of_ROS_in_midgut.xlsx
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Yana_L._Vorontsova_Figure_3d_Total_antioxidant_activity_im_midgut.xlsx
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Yana_L._Vorontsova_Figure_S1a.xlsx
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Yana_L._Vorontsova_Figure_S1b.xlsx
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Abstract
Insects’ defenses can be directed at both live and inactivated pathogens. Recognition of pathogen-associated structures leads to modulation of innate immune responses and oxidative stress in the host. The article presents results of experiments showing that inactivated vegetative cells of Bacillus thuringiensis, B. subtilis, and B. cereus can modulate immune responses in Galleria mellonella in the absence of damage to tissues of the midgut. In the first hours after feeding on Bacillus spp., an increase in the number of immunocompetent hemocytes was observed. Phenoloxidase activity in the hemolymph of wax moth larvae was inhibited, which is confirmed by the suppression of the production of reactive oxygen species in hemolymph. In addition, it was found that the antioxidant response to the bacterial inoculation in the midgut and hemolymph is different. Probably, the local antioxidant defense system is probably configured in such a way that the level of reactive oxygen species increases rapidly to perform useful biological functions and decreases just as quickly, minimizing oxidative damage.
Dataset DOI: 10.5061/dryad.jdfn2z3nz
Description of the data and file structure
Data set for the manuscript by Jana L. Vorontsova et al. “Effect of inactivated bacteria on the redox status of Galleria mellonella wax moth larvae”, including
(i) survival of G. mellonella wax moth larvae after per os administration of inactivated vegetative cells of B. thuringiensis, B. subtilis or B. cereus,
(ii) total hemocyte count (THC) (a) and phenoloxidase (PO) activity (b) in the hemolymph of G. mellonella wax moth larvae 12 and 24 h after per os administration of inactivated vegetative cells of B. thuringiensis, B. subtilis or B. cereus,
(iii) the magnitude of reactive oxygen species (ROS) production (a, c) and total antioxidant activity (TAC) (b, d) in the hemolymph (a, b) and midgut (c, d) of G. mellonella wax moth larvae 12 and 24 h after per os administration of B. thuringiensis, B. subtilis or B. cereus IBVCs, and
(iv) survival of G. mellonella wax moth larvae after per os administration of (a) active vegetative cells and (b) 6-day-old bacterial cultures of B. thuringiensis, B. subtilis or B. cereus.
Files and variables
- Yana_L._Vorontsova_Figure_1_The_survival_of_wax_moth.xlsx - Yana L. Vorontsova Figure 1 The survival of wax moth - The dataset for Figure 1 The survival of wax moth G. mellonella larvae after per os administration of inactivated vegetative cells of B. thuringiensis, B. subtilis, or B. cereus. Missing values (empty cells) will be marked with "n/a".
- Yana_L._Vorontsova_Figure_2a_The_total_hemocyte_count.xlsx - Yana L. Vorontsova Figure 2a The total hemocyte count - The dataset for Figure 2a The total hemocyte count (THC) (a) and activity of phenoloxidases (PO) (b) in the hemolymph of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or *B. cereus. *
- Yana_L._Vorontsova_Figure_2b_Activity_of_phenoloxidase.xlsx - Yana L. Vorontsova Figure 2b Activity of phenoloxidase - The dataset for Figure 2b The total hemocyte count (THC) (a) and activity of phenoloxidases (PO) (b) in the hemolymph of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or *B. cereus. *Missing values (empty cells) will be marked with "n/a".
- Yana_L._Vorontsova_Figure_3a_Generation_of_ROS.xlsx - Yana L. Vorontsova Figure 3a Generation of ROS - The dataset for Figure 3a The magnitude of production of reactive oxygen species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the midgut (c, d) of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or B. cereus.
- Yana_L._Vorontsova_Figure_3b_Total_antioxidant_activity_in_hemolymph.xlsx - Yana L. Vorontsova Figure 3b Total antioxidant activity in hemolymph - The dataset for Figure 3b The magnitude of production of reactive oxygen species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the midgut (c, d) of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or B. cereus.
- Yana_L._Vorontsova_Figure_3c_Generation_of_ROS_in_midgut.xlsx - Yana L. Vorontsova Figure 3c Generation of ROS in midgut - The dataset for Figure 3b The magnitude of production of reactive oxygen species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the midgut (c, d) of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or B. cereus.
- Yana_L._Vorontsova_Figure_3d_Total_antioxidant_activity_im_midgut.xlsx - Yana L. Vorontsova Figure 3d Total antioxidant activity im midgut - The dataset for Figure 3b The magnitude of production of reactive oxygen species (ROS) (a, c) and total antioxidant activity (TAC) (b, d) in hemolymph (a, b) and in the midgut (c, d) of wax moth G. mellonella larvae at 12 and 24 h after per os administration of IBVCs of B. thuringiensis, B. subtilis, or B. cereus. Missing values (empty cells) will be marked with "n/a".
- Yana_L._Vorontsova_Figure_S1a.xlsx - Yana L. Vorontsova Figure S1a - The dataset for Figure S1a The survival of G. mellonella wax moth larvae after per os administration of (a) active vegetative cells and (b) 6-day-old bacterial cultures of B. thuringiensis, B. subtilis or B. cereus.
- Yana_L._Vorontsova_Figure_S1b.xlsx - Yana L. Vorontsova Figure S1b - The dataset for Figure S1b The survival of G. mellonella wax moth larvae after per os administration of (a) active vegetative cells and (b) 6-day-old bacterial cultures of B. thuringiensis, B. subtilis or B. cereus.
Code/software
Microsoft Office (Excel)