The PtrRZFP4-PtrJAZ2 module regulates the jasmonic acid signaling pathway involved in the interaction between poplar and herbivorous insects
Data files
Sep 17, 2025 version files 13.61 KB
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Data1_Herbivore_bioassays_in_Fig.1.csv
1.58 KB
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Data2_Immunoblot_results_in_Fig._5.csv
1.98 KB
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Data3_Herbivore_bioassays_in_Fig._6.csv
3.19 KB
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Data4_Field_experiment_in_Fig._7.csv
1.34 KB
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Data5_Supplementary_Figures.csv
1.83 KB
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README.md
3.69 KB
Abstract
The plant hormone jasmonic acid (JA) is pivotal in regulating plant growth and defense mechanisms. Despite extensive research on the JA signaling network, the integration of other factors with JAZ (jasmonate ZIM-domain) proteins to modulate JA signaling in response to diverse herbivore attacks remains unclear. In this study, we employed molecular biology and multi - omics techniques to identify an E3 ubiquitin ligase, PtrRZFP4. This E3 ubiquitin ligase exerts a positive regulatory effect on the JA signaling pathway, yet demonstrates differential responses to distinct insect types. We found that upon insect attack, the expression of PtrRZFP4 transcripts is upregulated. In addition, PtrRZFP4 is able to interact with PtrJAZ2 and promote the ubiquitination and degradation of PtrJAZ2 protein. Furthermore, PtrRZFP4 activates the jasmonic acid (JA) signaling pathway at both the transcriptional and metabolic levels, triggering the synthesis of a large number of secondary metabolites related to insect resistance, such as terpenoids and alkaloids. This ultimately enhances the plant's defense against insect herbivory. The larvae of the specialist insect showed a strong preference for feeding on the leaves of transgenic poplar overexpressing PtrRZFP4 (OX-PtrRZFP4), which contain elevated levels of secondary metabolites. In contrast, the larvae of the generalist insect avoid leaves with increased secondary metabolite levels. However, PtrJAZ2 exhibits an opposite function to PtrRZFP4 in the resistance of poplar trees to different herbivores. Therefore, our study uncovers the novel role of the PtrRZFP4-PtrJAZ2 module in plant JA signaling and resistance to insect herbivory, highlighting its potential for biotechnological applications in improving insect resistance in forest trees.
Description of the Data and file structure
This study uncovers the novel role of the PtrRZFP4-PtrJAZ2 module in plant JA signaling and resistance to insect herbivory, highlighting its potential for biotechnological applications in improving herbivore resistance in forest trees.
The treatment codes were listed as below:
WT-wildtype
OX-PtrRZFP4-PtrRZFP4 gene overexpressed genotpyes
KO-PtrRZFP4-PtrRZFP4 gene knockout genotpyes
Files and variables
File: Data1_Herbivore bioassays in Fig.1.csv
Description: This file contains measurements of leaf area consumed (mm²) by larvae of the specialist herbivore Botyodes diniasalis and the generalist herbivore Spodoptera frugiperda when feeding on different plant genotypes. In addition, the increased fresh weight of individual larvae of the specialist herbivore Botyodes diniasalis and the generalist herbivore Spodoptera frugiperda fed on leaves of different plant genotypes were also recorded.
This dataset contains measurements of herbivore feeding leaf area and increased fresh weight of the specialist and generalist herbivores when they fed on leaves of different poplar genotypes
Variables
leaf feeding area(mm2): the consumed leaf area by herbivores
increased fresh weight(g): the growth biomass of herbivores fed on leaves of different poplar genotypes for seven days
File: Data2_Immunoblot results in Fig. 5.csv
Description: This file contains measurements of signal intensity of different poplar genotypes subjected to immunoblot
Variables
signal intensity: signal intensity of different poplar genotypes subjected to immunoblot
File: Data3_Herbivore bioassays in Fig. 6.csv
Description: This file contains measurements of leaf area consumed (mm²) by larvae of the specialist herbivore Botyodes diniasalis and the generalist herbivore Spodoptera frugiperda when feeding on different plant genotypes. In addition, the increased fresh weight of individual larvae of the specialist herbivore Botyodes diniasalis and the generalist herbivore Spodoptera frugiperda fed on leaves of different plant genotypes were also recorded. Moreover, the relative expression levels of specific genes in poplar leaves over time following different applications were detected according to q-RTPCR .
Variables
leaf feeding area(mm2): the consumed leaf area by herbivores
increased fresh weight(g): the growth of herbivores fed on leaves of different poplar genotypes
Relative expression level: relative expression levels of specific gene over time following different applications
File: Data4_Field experiment in Fig. 7.csv
Description: This file contains measurements of growth-related traits of different poplar genotypes grown in the field.
Variables
basal stem diameter(mm): stem growth of different poplar genotypes
plant height (cm): growth height of different poplar genotypes
leaf water content %: leaf water content of different poplar genotypes
leaf fresh weight (g): leaf biomass of different poplar genotypes
stem fresh weight (g): stem biomass of different poplar genotypes
pest index: herbivore damage on the leaves of different poplar genotypes
File: File: Data5_Supplementary Figures.csv
Description: This file contains measurements of relative gene expression levels of different poplar genotypes
Variables
relative expression level: relative gene expression level of different poplar genotypes in the q-rtPCR analysis
Sharing/access Information
Links to other publicly accessible locations of the data:
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Was data derived from another source?
No