Hawaiian caterpillar patrols spiderwebs camouflaged in insect prey’s body parts
Data files
Apr 24, 2025 version files 20.99 MB
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README.md
3.18 KB
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SM_CSI.zip
20.99 MB
Abstract
Lepidoptera is the most herbivorous of all the insect orders with predatory caterpillars globally comprising less than .13% of the nearly 200,000 moth and butterfly species. Here, we describe a species whose caterpillars are carnivorous inhabitants of spider’s webs, feeding on arthropods they find there. This Hawaiian lineage also boasts an unprecedented and macabre practice of decorating its portable larval home with the body parts of spider prey it harvests from the web where it resides. Phylogenomic data suggests the origin of this unique spider cohabitant is at least six million years old, over one million years older than Hawaii’s current high islands. After decades of searching, only one species has been discovered, restricted to 15 km2 on a single mountain range on the island of Oʻahu, meaning other members of the lineage have disappeared from older islands. Conservation action to save this globally unique lineage is imperative and overdue.
https://doi.org/10.5061/dryad.p8cz8wb1d
Description of the data and file structure
This dataset contains the scripts used in the analyses by Rubinoff et al. (in review). These scripts were used to process raw Illumina WGS reads from species of Hyposmocoma to extract loci used in Kawahara et al. (2019) for phylogenomic analysis. Once loci were extracted, we aligned loci using MAFFT and inferred phylogenetic trees using IQtree. Divergence time dating was conducted using MCMCtree in the PAML package.
Files and variables
File: SM_CSI.zip
Description of the data and file structure
This zip file contains scripts used to extract loci from raw WGS reads using hybpiper and conduct phylogenetic analysis using iqtree and MCMCtree.
Files and Folders
Hybpiper/01_clean_hybpiper.slurm
Script used to clean and run the assembly step of Hybpiper.
Hybpiper/02_hybpiper_stats.slurm
Script used to calculate assembly stats for Hybpiper.
Hybpiper/03_hybpiper_extract_sequences.slurm
Script used to extract loci.
Hybpiper/Akito_markers_DPLEX.AA_hybpip_fixed.fa
This file is the protein sequences from Kawahara et al (2019) from Danaus plexippus.
Hybpiper/CSI_mafft_aligned/*
This folder contains the mafft aligned loci extracted from Hybpiper using the Akito_markers_DPLEX.AA_hybpip_fixed.fa as baits.
Hybpiper/CSI_min_75_17/*
This folder contains the mafft aligned loci extracted from Hybpiper that passed filtering by sequence length and were present in all 17 Hyposmocoma samples.
IQTREE/
This folder contains the dataset, partition files, and output files from the IQtree run.
This file contains the partitions used to conduct partition analyses in IQTREE.
This file contains the best models for each partition used, found in the model finder implemented in IQTREE
This file contains the maximum likelihood tree found among the 10 runs of IQTREE.
MCMCTREE/
This folder contains the dataset, MCMC_Treefile, and config files for MCMCtree.
This file contains the sequence alignment used in MCMCtree and IQTREE
This file is the likelihood tree file from IQTREE used by MCMCtree for our dating analyses.
This file is the dated phylogeny inferred from MCMCtree.
This file is the config file for step 1 in the MCMCtree analysis.
This file is the config file for step 2 in the MCMCtree analysis.
Access information
Other publicly accessible locations of the data:
- NCBI Genbank Bioproject PRJNA1143550.
- Akito_markers_DPLEX.AA_hybpip_fixed.fa was derived from the following