Skip to main content
Dryad

BARCODE: high throughput screening and analysis of soft active materials

Valentine, Megan1; Chen, Qiaopeng1; Sriram, Aditya2; Das, Ayan1; Matic, Katarina2; Hendija, Maya2; Tonry, Keegan3; Ross, Jennifer4; Das, Moumita3; McGorty, Ryan2; Robertson-Anderson, Rae2

Published Dec 15, 2025 on Dryad. https://doi.org/10.5061/dryad.pc866t235

Data files

Dec 15, 2025 version files 339.53 KB

Click names to download individual files

Abstract

Active, responsive, non-equilibrium materials–at the forefront of materials engineering–offer dynamical restructuring, mobility, and other complex life-like properties. Yet, this enhanced functionality comes with significant amplification of the size and complexity of the datasets needed to characterize their properties, thereby challenging conventional approaches to analysis. To meet this need, we developed BARCODE: Biomaterial Activity Readouts to Categorize, Optimize, Design, and Engineer, an open-access software that automates high-throughput screening of microscopy video data to enable non-equilibrium material optimization and discovery. BARCODE produces a unique fingerprint or ‘barcode’ of performance metrics that visually and quantitatively encodes dynamic material properties with minimal file size. Using three complementary material-agnostic analysis branches, BARCODE significantly reduces data dimensionality and size, while providing rich, multiparametric outputs and rapid, tractable characterization of activity and structure. Here, we provide a series of datasets of cytoskeleton networks and cell monolayers that demonstrate BARCODE’s abilities to accelerate and streamline screening and analysis, reveal unexpected correlations and emergence, while showcasing BARCODE's ability to enable broad non-expert data access, comparison, and sharing. BARCODE software is available at https://github.com/softmatterdb/barcode.