Data from: Evidence for toxin-encoding coinfections driving intransitive dynamics between allelopathic phenotypes in natural yeast populations
Data files
Dec 12, 2025 version files 113.98 KB
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NZ_YeastMetapopulation_Metadata_ConservativeGenotyping_DRYAD.csv
103.15 KB
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README.md
2.41 KB
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TimeSpaceShiftExperimentData_Dryad.csv
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Abstract
The available datasets include natural yeast isolate survey data collected across three years, including microsatellite information and metadata, as well as data from a follow-up time-shift experiment designed to test whether the distributions of wild yeast isolates are influenced by whether or not they have resistance to the toxins produced by killer yeast in the population.
Dataset DOI: 10.5061/dryad.pk0p2nh35
Description of the data and file structure
Files and variables
File: NZ_YeastMetapopulation_Metadata_ConservativeGenotyping_DRYAD.csv
Description: Microsatellite genotype, infection state, and geographic data for 536 wild yeast isolates from vineyards across New Zealand in 2018, 2019 and 2021.
Variables
- AlternativeID - Unique identifier for all yeast isolates;
- Name - Unique identifier for all yeast isolates;
- FullName - Unique identifier for all yeast isolates;
- Year - The year the yeast isolate was sampled;
- Cultivar - Cultivar of grape that the yeast isolate was sampled from;
- Region - Region of New Zealand sampled from. Either Hawke's Bay or Marlborough;
- Vineyard - Identification code for the vineyard that the yeast isolate was sampled from;
- Latitude - The latitude of the vineyard from which the yeast isolate was sampled;
- Longitude - The longitude of the vineyard from which the yeast isolate was sampled;
- Genotype: Genotype assigned by customised genotype assignment algorithm;
- dsRNA infection assignment - virus-free (VF), totivirus infection (HVO) & totivirus-satellite coinfection (KV);
- 8 diploid microsatellite loci (16 columns, 2 for each microsatellite locus, including mating type loci);
File: TimeSpaceShiftExperimentData_Dryad.csv
Description: Time-space shift competition assays data. Killer yeast genotype competed against sympatric and allopatric competitors from the year it was present and the year it went extinct.
Variables
- PlateID - identification code for plate that assay was conducted on;
- Stamp - Killer genotype isolate code;
- Lawn - Competitor isolate code;
- Inhibition (binary response variable) - whether the killer genotype could kill the competitor or not;
- FocalMLG_Vineyard - Vineyard from which the focal killer yeast genotype was sampled from;
- Cohabitation - allopatric (competitor from different location) or sympatric (competitor from same location as killer genotype);
- Concurrence - Contemporary (competition sampled from the same year as the killer genotype) and Thereafter (competitor sampled from the year after the killer genotype went extinct);
Wild Saccharomyces cerevisiae isolates were collected from vineyards across New Zealand in 2018, 2019, and 2021. Colonies from wine ferments were picked and genotyped using microsatellite markers. Each isolate was then screened for dsRNA infections, focusing on totiviruses and toxin-encoding satellites. We first analyzed whether specific genotypes were associated with these toxin-encoding coinfections. Upon identifying a genotype that exhibited a significant decline across its entire range from one year to the next, we conducted time-shift killer assays. These assays tested whether competitors of the killer genotype in sympatric sites were resistant to the toxin, whether competitors in allopatric sites were susceptible, and whether isolates from sympatric sites, after the killer genotype had disappeared, were also sensitive, suggestive of a re-emergence of toxin sensitivity in the absence of the killer genotype.