Dryad DOI: https://doi.org/10.5061/dryad.sqv9s4nh5

We have submitted our raw data (PomaceaHH_3.csv and Villi_DATASET.csv).
In addition, we have submitted an R script (pomacea_tetra_3_PRLS.R ) and a Prism code (Morphology_analysis_villi.pzf)

Descriptions

PomaceaHH_3.csv

This matrix contains the spectral data from the egg clutches of three Pomacea snail species, plus two backgrounds on which the eggs are laid:

• wavelength
  nm: Wavelength in nanometers
• Pomacea samples include P. maculata, P. diffusa, P. scalaris, P. canaliculata; background samples include the leaf of Sagitaria montevidensis and rock substrate.
  species the first three letters of the column name code the species name epithet
  sample the first nine letters code the unique sample name
  replicate the 11th letter codes the replicate of each sample
• Background spectra are coded with the first three letters of the column name as:
  bkg with "saeta_" and "tosca_" indicating Sagitaria or rock substrates, respectively

Code/Software

R is required to run pomacea_tetra_3_PRLS.R; the script was created using version 4.5.1.
Annotations are provided throughout the script through 1) PREPARE ENVIRONMENT, 2) VISUALIZE SPECTRA, 3) VISUAL MODELING, and 4) MODEL CHROMATIC DIFFERENCES (CONTRASTS).

Prism file for statistical analyses of bird villi data

The file morphology analysis villi.pzf is a Prism file that includes the villi morphology dataset and the scripts used to perform the statistical analyses (Kruskal–Wallis test) and generate the plots shown in Figure S2, which shows the effect of ingestion of both Pomacea species on quail gut at different times.

bird vililii dataset

Users can access the raw data in the Villi_DATASET.csv file bird vililii dataset, but will need GraphPad PRISM to access statistics and graphics.

The units of both vilii width and vilii length are in micrometers (μm)