Assessment of Drosophila melanogaster pupae killed by heat as hosts for rearing Trichopria drosophilae
Data files
Oct 08, 2025 version files 393.93 KB
-
F0_data.xlsx
18.87 KB
-
F1_life_table.xlsx
129.23 KB
-
F2_life_table.xlsx
118.61 KB
-
F3_life_table.xlsx
124.26 KB
-
README.md
2.96 KB
Abstract
Trichopria drosophilae is a globally distributed pupal parasitoid that targets various species within the Drosophilidae family, including the invasive Drosophila suzukii. The mass rearing of T. drosophilae is a critical step in ensuring their successful application for field D. suzukii control. The pupae of D. melanogaster are currently used as a host for the mass rearing of T. drosophilae. After thermal treatment, the period during which pupae remain suitable for parasitism is extended, resulting in increased parasitism efficiency of the parasitoids. However, there have been no reports to date on the parasitism of heat–killed D. melanogaster pupae by T. drosophilae, and the effects of continuous rearing on heat-killed pupae on T. drosophilae remain unknown. To evaluate the utilization efficiency of heat–killed Drosophila pupae by T. drosophilae, we conducted a comprehensive analysis of key biological indicators across the F1, F2, and F3 generations. These included adult longevity, parasitism rate, female offspring production, changes in offspring sex ratio with maternal age, and population life table parameters. Our results showed that T. drosophilae can be continuously reared for multiple generations on heat–killed D. melanogaster pupae. Moreover, the T. drosophilae exhibits an adaptive response to heat–killed hosts, with population parameters declining in the F2 generation and recovering in the F3 generation. Using heat–killed pupae provides an efficient and economically viable method for the mass rearing of T. drosophilae, and offers valuable insights into its potential effectiveness in the field control of D. suzukii.
Dataset DOI: 10.5061/dryad.t1g1jwtf4
Description of the data and file structure
The file F0_data.xlsx contains the number of emerged offspring from the F0 generation in both the live group and the heat-killed group.
The file F1_life_table.xlsx contains the population life table parameters of the F1 generation for both the live group and the heat-killed group.
The file F2_life_table.xlsx contains the population life table parameters of the F1 generation for both the live group and the heat-killed group.
The file F3_life_table.xlsx contains the population life table parameters of the F1 generation for both the live group and the heat-killed group.
Files and variables
Basic Parameters
- x: Age (days).
- lxf: Survivorship of females at age x (%).
- lxm: Survivorship of males at age x (%).
- mxf: Age-specific fecundity of females at age x (individuals).
- mxm: Age-specific fecundity of males at age x (individuals).
- Mx: age-specific mean number of offspring produced per individual at age x (individuals).
Mortality and Survival Rates
- dxf: Age-specific mortality of females (%).
- dxm: Age-specific mortality of males (%).
- pxf: Probability of female survival from age x to x+1 (%).
- pxm: Probability of male survival from age x to x+1 (%).
- qxf: Probability of female death from age x to x+1 (%).
- qxm: Probability of male death from age x to x+1 (%).
Life Expectancy Calculation
- Lxf: Average number of surviving females at age x (individuals).
- Lxm: Average number of surviving males at age x (individuals).
- Txf: Total time lived by all females beyond age x (days).
- Txm: Total time lived by all males beyond age x (days).
- exf: Life expectancy of females at age x (days).
- exm: Life expectancy of males at age x (days).
Reproductive Value Calculation
- Lxf * mxf: Reproductive value of females at age x (dimensionless).
- x * lxf * mxf: Age-weighted reproductive output of females (dimensionless).
- lxf * mxf: Age-specific reproductive contribution of females (dimensionless).
Overall Life Table Parameters
- T: Generation time (days).
- R0: Net reproductive rate (dimensionless).
- r: Intrinsic rate of increase (dimensionless).
- EXP(-rm*x) * mxf * Lxf: Equation for calculating intrinsic rate of increase (dimensionless).
- rm: Numerical value of intrinsic rate of increase (dimensionless).
- DT: Doubling time of the population (days).
- λ: Finite rate of increase (dimensionless).
NOTE: Grey shading in each tab indicates empty cells.
Code/software
It is recommended to open the file using Microsoft Office Excel for the best compatibility and functionality.