Dataset DOI: 10.5061/dryad.tdz08kq9p

Description of the data and file structure

This dataset contains genetic and behavioral data related to the parasitic nematode Sphaerularia bombi and its bumble bee hosts (Bombus spp.). The dataset includes COI haplotypes, nuclear rRNA genotypes, microsatellite genotypes, and mark-recapture experimental data collected from multiple geographic locations.

Files and variables

DataFile.xlsx

This file contains all of the data sheets described below.

DataFile_Sheet1.csv: This sheet contains COI haplotype data for 542 Sphaerularia bombi individuals.

• Host_species: Host species of the S. bombi individual
• Host_ID: Unique ID of the host
• Site, Region, Country: Geographic location where the host was captured
• COI_haplotype: COI haplotype of the S. bombi individual

DataFile_Sheet2.csv : This sheet contains nuclear rRNA genotype data for 166 Sphaerularia bombi individuals from the 542 S. bombi individuals listed in Sheet 1: COI_Haplotype.

• Host_species: Host species of the S. bombi individual
• Host_ID: Unique ID of the host
• Site, Region, Country: Geographic location where the host was captured
• COI_haplotype: COI haplotype of the S. bombi individual
• nuc_allele_1, nuc_allele_2: Nuclear rRNA genotype of the S. bombi individual. nuc_allele_2 is recorded only if the individual is heterozygous.

DataFile_Sheet3.csv: This sheet contains the accession numbers assigned by the DNA Data Bank of Japan (DDBJ) for each COI haplotype and nuclear rRNA genotype.

DataFile_Sheet4.csv: This sheet contains microsatellite (simple sequence repeat: SSR) genotype data for 354 bumble bee queen individuals.

• Species: Bumble bee species
• Bee_ID: Unique ID of the bee
• Site, Region, Country: Geographic location where the bee was captured
• Loc1_1, Loc1_2, etc.: SSR genotype for each SSR locus

DataFile_Sheet5.csv: This sheet contains information on the Bombus microsatellite markers used.

References

Stolle E, Rohde M, Vautrin D et al. (2009) Novel microsatellite DNA loci for Bombus terrestris (Linnaeus, 1758). Molecular Ecology Resources, 9, 1345–1352.

Stolle E, Wilfert L, Schmid-Hempel R et al. (2011) A second generation genetic map of the bumblebee Bombus terrestris (Linnaeus, 1758) reveals slow genome and chromosome evolution in the Apidae. BMC Genomics, 12, 48.

Estoup A, Solignac M, Cornuet JM, Goudet J, Scholl A (1996) Genetic differentiation of continental and island populations of Bombus terrestris (Hymenoptera: Apidae) in Europe. Molecular Ecology, 5, 19–31.

DataFile_Sheet6.csv: This sheet contains data from the mark-recapture experiment conducted at Site A.

• Year, Month, Date, Hour, Min: The date and time when the bumble bee individual was observed
• Species, Caste: The species and caste of the bumble bee
• Bee_ID: Unique identifier for the bee
• New_record or Recapture:
  • "New_record" if the individual was captured for the first time
  • "Recapture" if the individual had been captured previously
  • "Unknown" for individuals that were either unsuccessfully captured (Bombus diversus) or not captured (for species other than B. diversus)
• Released_location:
  • "Specific location within the survey area" if the individual was released within the designated survey area
  • "300 meters away from the survey area" if the individual was released at a distant location
  • Left blank if the individual was released at the observation site or not captured
• Behavior: The behavior of the bumble bee at the time of observation

DataFile_Sheet7.csv: This sheet contains data from the mark-recapture experiment conducted at Site B.

• Year, Month, Date, Hour, Min: The date and time when the bumble bee individual was observed
• Species, Caste: The species and caste of the bumble bee
• Bee_ID: Unique identifier for the bee
• Coordinate_x, Coordinate_y: The capture location coordinates of the bee
• New_record or Recapture:
  • "New_record" if the individual was captured for the first time
  • "Recapture" if the individual had been captured previously
  • "Unknown" for individuals that were either unsuccessfully captured (Bombus terrestris) or not captured (for species other than B. terrestris)
• Released_location:
  • "Specific location within the survey area" if the individual was released within the designated survey area
  • Left blank if the individual was released at the observation site or not captured
• Behavior: The observed behavior of the bumble bee at the time of observation