Data from: Dual pH-and thermoresponsive nano-objects with tunable morphologies and dye encapsulation prepared via aqueous emulsion PISA
Data files
Feb 23, 2026 version files 30.17 MB
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cryoTEM_Fig._3_(A).tif
3.06 MB
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cryoTEM_Fig.1_(A).tif
3.15 MB
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cryoTEM_Fig.1_(C).tif
3.33 MB
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cryoTEM_Fig.1_(E).tif
2.90 MB
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cryoTEM_Fig.1_(G).tif
3.25 MB
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cryoTEM_Fig.3_(B).tif
3.15 MB
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cryoTEM_Fig.3_(C).tif
3.10 MB
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cryoTEM_Figure_S6.tif
3.03 MB
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DLS_Figure_2_(B).opju
384.32 KB
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DLS_Figure_3_(D).opju
167.97 KB
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DLS_Figure_S5.opju
235.77 KB
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DLS_Figure_S7.opju
77.69 KB
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DLS_Figure2.(A).opju
324.46 KB
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NMR_Figure_S1.txt
1.32 MB
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NMR_Figure_S2.txt
1.40 MB
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NMR_Figure_S3.txt
1.14 MB
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PTEGMA120-PDPA135_SAXS.txt
6.82 KB
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PTEGMA120-PDPA265_SAXS.txt
6.82 KB
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PTEGMA120-PDPA265-NR_25C_SAXS.txt
13.73 KB
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PTEGMA120-PDPA265-NR_65C_SAXS.txt
13.73 KB
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PTEGMA120-PDPA35_SAXS.txt
6.82 KB
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PTEGMA120-PDPA75_SAXS.txt
6.82 KB
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README.md
3.76 KB
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SEC_-_Figure_S4.opju
110.54 KB
Abstract
Poly(triethylene glycol methyl ether methacrylate)-b-poly [2-(diisopropylamino)ethyl methacrylate] (PTEGMAb-PDPA) nano-objects, synthesized via polymerization-induced self-assembly (PISA), exhibit dual thermo-and pH-responsive behavior. The thermoresponsiveness originates from the lower critical solution temperature (LCST) of the PTEGMA block, while the pH-responsiveness arises from protonation of the PDPA block under acidic conditions. The hydrophilic PTEGMA block (degree of polymerization, DP = 120) was first synthesized to serve as a macro-chain transfer agent. This was followed by RAFT-mediated PISA emulsion polymerization of the pH-sensitive PDPA block (DP = 35-265) in aqueous solution at a solid content of 10 wt%. During polymerization, the block copolymers self-assembled in situ into diverse and tunable nanostructures, including micelles, branched worms, "jellyfish", and thick-walled vesicles. These nano-objects were thoroughly characterized using cryo-transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), and small-angle X-ray scattering (SAXS). The in situ drug encapsulation capability of these dual-responsive nanoformulations was demonstrated using Nile Red (NR), a hydrophobic fluorescent model compound. Specifically, thick-walled vesicles (PTEGMA 120 -b-PDPA 265 ) successfully encapsulated NR during RAFT-PISA synthesis and released it under acidic conditions and/or at mildly elevated temperatures. These results highlight the potential of tailorable PTEGMA-b-PDPA nano-objects as "smart" nanocarriers for targeted and stimuli-responsive drug delivery applications.
Dataset DOI: 10.5061/dryad.v41ns1sb1
Description of the data and file structure
This folder contains raw DLS, cryo-TEM, NMR, SEC, and SAXS data forthe article titled "Dual pH- and Thermoresponsive Nano-Objects with Tunable Morphologies and Dye Encapsulation Prepared via Aqueous Emulsion PISA."
Files and variables
File: PTEGMA120-PDPA35_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA35
Variables
- Q/nm -1
- Intensity/a.u.
File: PTEGMA120-PDPA75_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA75
Variables
- Q/nm -1
- Intensity/a.u.
File: PTEGMA120-PDPA265_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA265
Variables
- Q/nm -1
- Intensity/a.u.
File: PTEGMA120-PDPA265-NR_65C_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA265-NR at 65 °C
Variables
- Q/nm -1
- Intensity/a.u.
File: PTEGMA120-PDPA135_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA135
Variables
- Q/nm -1
- Intensity/a.u.
File: PTEGMA120-PDPA265-NR_25C_SAXS.txt
Description: SAXS raw data for the sample PTEGMA120-PDPA265-NR at 25 °C
Variables
- Q/nm -1
- Intensity/a.u.
File: NMR_Figure_S3.txt
Description: proton NMR raw data for the sample PTEGMA120-b-PDPA265
Variables
- Chemical shift / ppm
- Frequency / Hz
File: NMR_Figure_S2.txt
Description: proton NMR raw data for the sample PTEGMA
Variables
- Chemical shift / ppm
- Frequency / Hz
File: NMR_Figure_S1.txt
Description: proton NMR raw data for the sample CETPA
Variables
- Chemical shift / ppm
- Frequency / Hz
File: SEC_-_Figure_S4.opju
Description: Origin project containing raw SEC data for this manuscript
File: cryoTEM_Fig.3_(B).tif
Description: raw cryo-TEM picture for PTEGMA120-PDPA265-NR at 65C
File: cryoTEM_Fig.3_(C).tif
Description: raw cryo-TEM picture for PTEGMA120-PDPA265-NR at pH 5.5
File: cryoTEM_Figure_S6.tif
Description: raw cryo-TEM image of PTEGMA125-b-PDPA135
File: cryoTEM_Fig._3_(A).tif
Description: raw cryo-TEM picture for PTEGMA120-PDPA265-NR at 25 °C
File: cryoTEM_Fig.1_(G).tif
Description: PTEGMA120-PDPA265
File: cryoTEM_Fig.1_(C).tif
Description: PTEGMA120-PDPA75
File: cryoTEM_Fig.1_(E).tif
Description: PTEGMA120-PDPA135
File: cryoTEM_Fig.1_(A).tif
Description: PTEGMA120-PDPA35
File: DLS_Figure_2_(B).opju
Description: Origin project containing raw DLS data for temperature dependence of the DH of PTEGMA120-b-PDPAn nano-objects
File: DLS_Figure2.(A).opju
Description: Origin project containing raw DLS data for temperature dependence of pH-responsive behavior
File: DLS_Figure_S7.opju
Description: Origin project containing raw DLS data for temperature dependence of the DH of PTEGMA120-b-PDPA265-NR Vs
File: DLS_Figure_S5.opju
Description: Origin project containing raw DLS data for particle size distributions obtained for PTEGMA120-b-PDPAn (n = 35, 75, 135, and 265)
File: DLS_Figure_3_(D).opju
Description: Origin project containing raw DLS data for PTEGMA120-b-PDPA265-NR at 25 °C, 40.1 °C and under acidic conditions (pH = 5.5)
Code/software
SAXS and NMR data can be opened using Notepad.
DLS and SEC data can be opened using Origin.
cryo-TEM pictures can be opened using IrfanView.