Data from: An antagonistic epigenetic mechanism regulating gene expression in pollen revealed through single-nucleus multiomics
Data files
Oct 23, 2025 version files 7.04 GB
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Microscopy_Data_MBD6_and_MBD7_Antagonism.zip
7.04 GB
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README.md
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Abstract
MBD6 and MBD7 complexes compete against one another for gene silencing and activation, however, the mechanisms are poorly understood. These microscopy results support other experiments in our manuscript that demonstrate MBD6 and MBD7 compete with one another DNA methylation sites, accumulate at DNA methylation using similar mechanisms, and antagonize one another at those sites. Furthermore, we express MBD7 using its endogenous promoter and reveal a unique localization pattern to primarily around the chromocenters near the nucleolus and not always to all chromocenters as reported previously.
https://doi.org/10.5061/dryad.vx0k6dk23
Description of the data and file structure
All microscopy was collected using an LSM 980 confocal microscope with either 2-3 week old seedlings, focused on roots, or on tobacco leaf tissue.
Files and variables
File: Microscopy_Data_MBD6_and_MBD7_Antagonism.zip
Description:
Files of Arabidopsis gene expression constructs are included in the Zip file and named by the genetic background (e.g. Col-0 (WT), mbd5 mbd6, mbd7, idm2 (lil) mutants etc.) followed by the construct description of promoter used and the gene and fluorescent tag (e.g. MBD7 promoter with MBD7 YFP), followed by supporting information (date of experiment, replicate, etc.).
Files of bimolecular fluorescence (BiFC) assay the constructs are labelled with BiFC, then the protein attached to the N-terminal half of YFP (N) followed by the protein that is attached to the C-terminal half of YFP (C), plant number imaged, and leaf number or if DAPI was used on the sample (e.g. BiFc_MBD6 C term Deletion (N) with ACD15 (C)_Plant 1_leaf1.1.czi).
To open files use either ZEN software for ImageJ (FIJI). Data includes both z-stacks and single images.
All of this data was collected using an LSM 980 confocal microscope and across experiments laser settings and magnifications were kept constant. All experiments were performed on live plant tissue either arabidopsis 2-3 week old seedling roots or tobacco leaves.