Article Title: Sexually dimorphic and clock-gene specific effects of artificial light at night on Drosophila behavioral rhythms (2025)
Pranevicius A., Biondi G., Iyer A.R., Seiffe A., Depino A.M., and Fernandez M.P.

Journal: Proceedings of the Royal Society B
Dataset DOI: 10.5061/dryad.wwpzgmswz
Contact: Maria de la Paz Fernandez (fernanm@iu.edu)

Description of the Data and File Structure

• All data files are in .csv format.
• Each file includes descriptors for sex, environmental condition (and genotype, when applicable). All experiments were conducted under a 12:12 light-dark cycle with either complete darkness at night (LD) or dim white light (ALAN). Zeitgeber time 0 (ZT12) indicates lights-on transition and Zeitgeber time 12 (ZT12) indicates lights-off transition (under LD) or start of the dim light phase (ALAN). Circadian time 0 (CT0) indicates the start of the subjective day and Circadian Time 12 (CT12) the start of the subjective night, under constant temperature and darkness (DD).
• Empty cells in the files that contain behavior data (all figures except Figure 2) indicate that either there was not a fly in the corresponding channel of the DAM activity monitor, or that it died before the end of the experiment.
• "n/a" entries in immunohistochemistry data (Figure 2) indicates no datapoint (not the exact same number of brains was imaged for all timepoints and conditions).

Figure 1

• Fig1b_Dryad.csv : Night to day activity
  This file contains locomotor activity data measured as beam crosses per minute in male and female Drosophila. Flies were either kept in standard light-dark (LD) conditions or exposed to artificial light at night (ALAN). The data is a ratio of diurnal (ZT0-12) over nocturnal (ZT12-24) activity, labeled in the figure as ‘nocturnality’. The genotype in Figure 1 data is w1118.
• Fig1c_Dryad.csv: Evening phase
  Phase of evening activity (in hours) relative to lights-off (ZT12) for males and females under LD or ALAN.
• Fig1e_Dryad.csv : Total sleep
  Total sleep duration (in minutes) for males and females under LD or ALAN.
• Fig1f_Dryad.csv : Day and night sleep
  Day (ZT0-12) vs. night (ZT12-24) sleep durations for males and females under LD or ALAN.
• Fig1g_Dryad.csv : Morning and evening sleep
  Sleep during ZT0–3 (morning) and ZT9–12 (evening) for males and females under LD or ALAN.

Figure 2

• Fig_2b_Dryad.csv : sLNv TIM timecourse
  TIMELESS (TIM) nuclear staining in the small lateral ventral neurons (sLNvs) at the timepoints ZT2, 6, 10, 14, 18, and 22. Intensity in arbitrary units (a.u).
• Fig_2c_Dryad.csv : sLNv PER timecourse
  PERIOD (PER) nuclear staining in the sLNvs at ZT10 and ZT22. Intensity in arbitrary units (a.u).
• Fig_2g_Dryad.csv : E3 PER timecourse
  PER staining in the E3 cluster of lateral neurons (LNds) for two antiphasic timepoints, ZT10 and ZT22. Intensity in arbitrary units (a.u).

Figure 3

• Fig_3a_Dryad.csv: Nocturnality in clock mutant males
  This file contains locomotor activity data measured as beam crosses per minute in male Drosophila of three different genotypes (wild type CS, per01, and cyc01) under LD and ALAN conditions. Similar to Fig 1b.
• Fig_3c_Dryad.csv: Nocturnality in clock mutant females
  Same as 3a but in females.
• Fig_3e_Dryad.csv: Sleep in clock mutant males
  Day and night sleep durations (minutes) in males of the same genotypes described in 3a.
• Fig_3f_Dryad.csv: Sleep in clock mutant females
  Same as 3e but in females.

Figure 4

• Fig_4a_Dryad.csv: Nocturnality, per CRISPR knockout males
  This file contains locomotor activity data measured as beam crosses per minute in male Drosophila for three genotypes: clk856-Gal4 and UAS-per-gs;UAS-Cas9 parental controls and experimental Clk856>per-gs+Cas9 flies under LD or ALAN conditions. Similar to Fig 1b.
• Fig_4d_Dryad.csv: Nocturnality, per CRISPR knockout females
  Same as 4a but in females.
• Fig_4c_Dryad.csv: Sleep, per CRISPR knockout males
  Day and night sleep (in minutes) for same genotypes described in 4a for males and females under LD or ALAN.
• Fig_4f_Dryad.csv: Sleep, per CRISPR knockout females
  Same as 4c but in females.

Supplementary Figure 1

• Fig_S1b_Dryad.csv : Morning phase
  Morning activity phase (in hours) relative to the lights-on transition (ZT0) for males and females under LD or ALAN.
• Fig_S1c_Dryad.csv : Sleep bout length
  Average bout duration (in minutes) for day and night sleep for males and females under LD or ALAN.
• Fig_S1d_Dryad.csv: Sleep latency
  Time to the first sleep bout (in minutes) for males and females under LD or ALAN.

Supplementary Figure 2

• Fig_S2b_Dryad.csv: Subjective day sleep
  Sleep (in minutes) for the first subjective day (Circadian Time, CT0-12) relative to the amount of sleep during the last day of either LD or ALAN (Zeitgeber Time, ZT0-12) for males and females.
• Fig_S2c_Dryad.csv: Total PER in sLNvs
  PER (nuclear + cytoplasmic) intensity in the sLNvs at ZT10 and ZT22 for males and females under LD or ALAN.

Supplementary Figure 3

• Fig_S3c_Dryad.csv: Total sleep
  Total sleep (in minutes) in CS, per01, and cyc01 males and females under LD or ALAN.

Supplementary Figure 4

• Fig_S4a_Dryad.csv: Total sleep
  Total sleep (in minutes) in clk856-Gal4, UAS-per-gs;UAS-Cas9, and Clk856>per-gs+Cas9 males and females under LD or ALAN.

Genotype Key:

• UAS control: UAS-per-gs;UAS-Cas9
• Gal4 control: Clk856-Gal4
• Experimental: Clk856>per-gs + Cas9