The impact of different energy sources on redox balance during endometriotic cyst laparoscopy
Data files
Nov 10, 2025 version files 34.67 KB
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Dataset.xlsx
29.34 KB
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README.md
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Abstract
Endometriosis is a common gynecological disorder seen in women worldwide. It reduces fertility with significantly changes a woman's quality of life. Laparoscopy proved to be standard treatment for endometriosis, particularly for endometriomas. Disruption of oxidative stress balance has been put in the focus in the recent studies. The aim of the study was to follow the impact of cystectomy and compare bipolar and laser electrocoagulation on prooxidative stress biomarkers and antioxidative capacity. A total of 60 women with diagnosed endometriosis were included in the study and divided in two groups according to laparoscopy treatment with different energy sources for electrocoagulation. Prooxidative parameters (superoxide anion radical (O2-), nitrites (NO2-), hydrogen peroxide (H2O2), index of lipid peroxidation measured as TBARS) and antioxidative defence system parameters (reduced glutathione (GSH), activity of superoxide dismutase (SOD), activity of catalase (CAT)) were determined spectrophotometrically before and after the surgery. The value of H2O2 was decreased in group treated with biporal electrocoagulation, while the value of GSH was increased in both groups. The value of O2- was significantly decreased in the group treated with laser while SOD activity was increased in the same group. The both methods had a similar effect on index of lipid peroxidase and reduced glutathione, but when it comes to the decreasing of harmful superoxide anion radical and increasing of SOD activity, laser electrocoagulation showed better outcome. The role of oxidative stress in both, understanding the pathogenesis of this disease and as a potential therapeutic target, is certainly of great importance.
This dataset contains biochemical and hormonal assay data comparing the effects of bipolar cystectomy and laser cystectomy on oxidative stress and endocrine function in human subjects. The study aimed to determine whether different surgical techniques influence oxidative stress and hormonal balance differently in the postoperative period.
Authors and Affiliations
Igor Gogic¹², Vladimir Jakovljevic³⁴⁵, Marina Nikolic³⁴, Sasa Raicevic¹⁶, Aleksandra Stojanovic⁴⁷
¹ Department of Gynecology and Obstetrics, Faculty of Medicine, University of Montenegro, Podgorica, Montenegro
² Department of Gynecology and Obstetrics, Special Codra Hospital, Podgorica, Montenegro
³ Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
⁴ Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia
⁵ Department of Human Pathology, First Moscow State Medical University I.M. Sechenov, Moscow, Russian Federation
⁶ Clinic of Gynecology and Obstetrics, Clinical Center of Montenegro, Podgorica, Montenegro
⁷ Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
Description of the data and file structure
The dataset is organized as an Excel file (Dataset.xlsx) containing 11 sheets, each representing a distinct biomarker or variable measured before and after surgery.
| Sheet Name | Variable Measured (with Units) | Description |
|---|---|---|
TBARS |
Thiobarbituric Acid Reactive Substances (𝜇mol/min/g wt) | Marker of lipid peroxidation and oxidative stress |
NO2 |
Nitrite (NO₂⁻) (nmol/min/g wt) | Indicator of nitric oxide metabolism |
O2 |
Oxygen consumption (nmol/min/g wt) | Reflects oxidative activity |
H2O2 |
Hydrogen peroxide (nmol/min/g wt) | Reactive oxygen species concentration |
CAT |
Catalase (U/g Hb×103) | Antioxidant enzyme activity |
SOD |
Superoxide dismutase (U/g Hb×103) | Antioxidant enzyme activity |
GSH |
Reduced glutathione (nmol/mL RBCs) | Non-enzymatic antioxidant |
FSH |
Follicle-Stimulating Hormone (IU/L) | Reproductive hormone concentration |
Estradiol |
Estradiol (E₂) (pg/mL) | Female sex hormone concentration |
AMH |
Anti-Müllerian Hormone (ng/mL) | Marker of ovarian reserve |
Age |
Participant ages (years) | Age data for both surgical groups |
Column structure in most sheets:
Bipolar_BS: Measurements before surgery (Bipolar group)Bipolar_AS: Measurements after surgery (Bipolar group)Laser_BS: Measurements before surgery (Laser group)Laser_AS: Measurements after surgery (Laser group)
Each row represents one participant.
There are 30 participants per group, and there is no missing data in this dataset.
Measurement units correspond to the standards of biochemical assay reporting listed above.
Experimental context
Human plasma and serum samples were analyzed from participants undergoing either bipolar or laser cystectomy.
- Oxidative stress biomarkers (TBARS, NO₂⁻, O₂, H₂O₂, CAT, SOD, GSH) were measured before surgery and within 24 hours after surgery.
- Endocrine parameters (FSH, Estradiol, AMH) were measured before surgery and six months after cystectomy, between days 3–5 of the menstrual cycle.
This design enabled comparison of short-term oxidative stress response and long-term effects on ovarian reserve between the two surgical techniques.
Sharing/Access information
This dataset is openly available via Dryad at:
🔗 https://doi.org/10.5061/dryad.xd2547dwb
Other publicly accessible sources:
- None
Data derivation:
All data were collected as part of original experimental measurements. No external or previously published datasets were used.
Code/Software
No executable code is included in this dataset.
Statistical analyses and visualizations were performed using GraphPad Prism 9.
No custom scripts were required for data preprocessing.
Suggested citation
> Gogic I, Jakovljevic V, Nikolic M, Raicevic S, Stojanovic A. (2025). THE IMPACT OF DIFFERENT ENERGY SOURCES ON REDOX BALANCE DURING ENDOMETRIOTIC CYST LAPAROSCOPY Dryad Digital Repository. https://doi.org/10.5061/dryad.xd2547dwb