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Dryad

Full-length transcriptomes of 25 grassland plant species

Jiang, Chongyi1; Wolf, Jochen2; Schielzeth, Holger1

Published Apr 17, 2025 on Dryad. https://doi.org/10.5061/dryad.z08kprrpv

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Abstract

Grasslands are essential, biodiverse ecosystems of economic importance that play a critical role for carbon storage and soil health. Despite their ecological and economic importance, transcriptomic resources for wild grassland species facilitating eco-evolutionary and functional genomic studies remain limited. In this study, we present full-length transcriptomes for shoot tissue of natural accessions of 25 wild grassland plant species collected from the field site of a long-term grassland biodiversity experiment (Jena Experiment). Using PacBio Iso-Seq technology, we generated a total of 522.45 million subreads which were assembled into isoforms for each species separately. This resulted in an average of 49,180 isoforms per species of which 68.6% were successfully annotated against the Swiss-Prot database. Fifty-six percent of the transcripts had complete open reading frames (ORFs), and 29.6% of the transcripts have been identified as non-coding RNAs (ncRNAs) by two prediction tools. This dataset provides a valuable full-length transcriptomic resource for exploring gene expression, alternative splicing, and evolutionary patterns in wild grassland plant species, paving the way for future functional genomics and conservation studies.