Dataset DOI: 10.5061/dryad.zcrjdfnsm

Description of the data and file structure

This dataset contains all the data used to generate the figures and supplementary figures in the above manuscript. The study investigates developmental and adult alterations of the medial prefrontal cortex (mPFC)–thalamic circuit in Shank3–/– mice, a model of autism spectrum disorder.

The dataset is organized into multiple Excel tabs, where each tab corresponds to a specific figure or supplementary figure. Both male and female mice are included, and statistical tests (including normality testing and resulting p-values) are provided within the file.  For any queries regarding the data, please contact the author, Gabrielle Devienne [devienne@stanford.edu]

Files and variables

File: Devienne_2025_Manuscript_DataPerFigures.xlsx

Description: Excel file with tabs per figure, figure panels, or supplementary figure data and metadata information

Each figure tab includes a table with the following metadata (when applicable):

• Animal ID
• Cage number
• Sex
• Date of Birth (DOB)
• Date of Experiment (DOE)
• Genotype (WT or Shank3)
• Raw data file name (from which experimental data were derived)

File Organization

All data are provided in a single Excel workbook, where:

• Each main figure corresponds to one primary tab.
• Associated supplementary figures.
• Raw file names are included to link each row to the original acquisition file.

Content Summary by Figure

Figure 1, Figure1_USV, Supplementary Figure 1 — Behavioral Data

Ultrasonic vocalizations (USVs) recorded at P11 using Avisoft Bioacoustics.
Parameters include:

• Call duration, interval, break, DistToMax
• Start/End/Centre/Max peak frequency, amplitude, and entropy
• Real-time recording timestamp
• Reject flag (for Matlab plotting/analysis purposes) 

Supplementary Figure 1 contains call-type categorization and statistics.

Adult social interaction behavior analyzed using ezTrack is also included.

Figure 2 & Supplementary Figure 2 — CSD Analysis

Current source density (CSD) measurements from mPFC slices recorded with TDT hardware and analyzed in MATLAB.
Values represent peak amplitude (µA/mm³) for:

• L2/3 presynaptic sink
• L2/3 postsynaptic source
• L5 presynaptic source
• L5 postsynaptic sink

Electrical stimulation intensities range from 100–800 µA.

Figure 3 & Supplementary Figure 4/7 — Patch-Clamp Electrophysiology (L2/3 and L5 Pyramidal Neurons)

Includes metadata (genotype, age, intracellular/extracellular solution), action potential properties, firing responses to depolarizing steps (–100 to +400 pA), and excitatory/inhibitory (E/I) ratios based on EPSCs at –70 mV and IPSCs at +10 mV.

Supplementary Figure 3 provides additional intrinsic properties: rheobase, AHP, threshold, AP width, AP overshoot, sag, and rebound.

• Sholl morphology analysis (Supplementary Figure 4)
• Supplementary Figure 7: E/I measurements using cesium-based internal solution (L2/3 and L5).

Figure 4 & Supplementary Figure 8 — Patch-Clamp in mediodorsal thalamus (MD)-Projecting mPFC Neurons

Adult mPFC neurons retrogradely labeled with retrobeads projecting to the MD thalamus.
Includes:

• Cell metadata (RMP, Ri, rheobase, acquisition rate)
• Spiking profiles
• Supplementary Figure 8: Action potential properties for cells recorded in Figure 4.

Figure 5 & Supplementary Figure 9 — MD Neuron Responses to Optogenetic Stimulation

Whole-cell voltage-clamp of MD thalamic neurons stimulated via mPFC ChR2-expressing axons at:

• 1 Hz
• 10 Hz (10-pulse train)

Supplementary Figure 9 contains additional 1 Hz current traces.

Figure 6 — Persistent Current Analysis

Persistent currents during 10 Hz trains in adult WT and Shank3–/– mice under:

• AP5 (NMDA receptor antagonist)
• DHPG (mGluR1/5 agonist)

Software & Acquisition Tools

• Avisoft Bioacoustics (USV recording and analysis)
• ezTrack (behavior analysis) (https://github.com/denisecailab/ezTrack)
• TDT system (slice electrophysiology acquisition, LFP/CSD)
• MATLAB (CSD and electrophysiology data processing)
• GraphPad Prism for analysis and statistics
• ImageJ / SNT plugin (morphology analysis)
• pClamp / Axon Instruments (patch clamp acquisition