Skip to main content
Dryad

Data from: Stability and gene strand bias of lambda prophages and chromosome organization in Escherichia coli

Stavans, Joel1; Goren, Rinat1; Dassa, Bareket1; Gallardo, Oscar1; Court, Donald2; Li, Xintian2

Published May 27, 2024 on Dryad. https://doi.org/10.5061/dryad.zgmsbcck8

Data files

Abstract

Temperate phage-mediated horizontal gene transfer is a potent driver of genetic diversity in the evolution of bacteria. Most lambdoid prophages in E. coli are integrated into the chromosome with the same orientation with respect to the direction of chromosomal replication, and their location on the chromosome is far from homogeneous. To understand better these features, we studied the interplay between lysogenic and lytic states of phage lambda in both native and inverted integration orientations, at the wild-type integration site, as well as at other sites on the bacterial chromosome. Measurements of free phage released by spontaneous induction showed that the stability of lysogenic states is affected by location and orientation along the chromosome, with stronger effects near the origin of replication. Competition experiments and range expansions between lysogenic strains with opposite orientations and insertion loci indicated that there are no major differences in growth. Using single-molecule fluorescence in situ hybridization we quantified the level of transcriptional bursts of the cI gene coding for the lambda phage repressor. These measurements indicate that the stability of the lysogenic states at native and inverted orientations are different. We postulate that the preference for a given orientation and location is a result of a balance between the maintenance of lysogeny and the ability to lyse. Together, our findings shed light on key mechanisms that determine the genomic architecture in the bacterial chromosome.