Dryad Digital Repository > Main > Dryad Data Packages > View Item

Data from: The role of pleiotropy in the maintenance of sex in yeast

When using this data, please cite the original article:

Hill JA, Otto SP (2007) The role of pleiotropy in the maintenance of sex in yeast. Genetics 175: 1419-1427. doi:10.1534/genetics.106.059444

Additionally, please cite the Dryad data package:

Hill JA, Otto SP (2007) Data from: The role of pleiotropy in the maintenance of sex in yeast. Dryad Digital Repository. doi:10.5061/dryad.18
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Dryad Package Identifier doi:10.5061/dryad.18 603 views 
Abstract In facultatively sexual species, lineages that reproduce asexually for a period of time can accumulate mutations that reduce their ability to undergo sexual reproduction when sex is favorable. We propagated Saccharomyces cerevisiae asexually for ~800 generations, after which we measured the change in sexual fitness, measured as the proportion of asci observed in sporulation medium. The sporulation rate in cultures propagated asexually at small population size declined by 8%, on average, over this time period, indicating that the majority of mutations that affect sporulation rate are deleterious. Interestingly, the sporulation rate in cultures propagated asexually at large population size improved by 11%, on average, indicating that selection on asexual function effectively eliminated most of the mutations deleterious to sporulation ability. These results suggest that pleiotropy between mutations’ effects on asexual fitness and sexual fitness was predominantly positive, at least for the mutations accumulated in this experimental evolution study. A positive correlation between growth rate and sporulation rate among lines also pro- vided evidence for positive pleiotropy. These results demonstrate that, at least under certain circum- stances, selection acting on asexual fitness can help to maintain sexual function.
Date Deposited 2007-12-14T22:27:55Z
Show Full Metadata
Maximum Likelihood Estimator 59 downloads View File Details
Mathematica file that summarizes and plots results of the program mlgenomeu.c to infer maximum likelihood estimates of the mutation rate and effect size, using two fitness assays (sporulation rate, growth rate).
Download: MLanalysis.nb ( 357.2Kb )
The following license files are associated with this item:   License

Statistical Analyzer 45 downloads View File Details
Mathematica file that contains all of the growth rate data obtained from the Bioscreen as well as all of the sporulation data. This file performs all of the major statistical analyses on these datasets reported in Hill and Otto (2007).
Download: sliding window 06 NEW.nb ( 7.235Mb )
The following license files are associated with this item:   License

Sporulation Rate Data 81 downloads View File Details
Download: sporedata.xls ( 174.0Kb )
The following license files are associated with this item:   License

My Account

Browse

Information