Data from: Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): Insights into resistance evolution in a hexaploid species

 

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Title Fig. 5. Plastidic ACCase gene partial sequence alignment
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Description Partial sequence alignment (from cloned fragments) showing the three haplotype sequences of the 2088, 1781 and 2078 mutations identified in the present study, corresponding respectively to the ACCase gene copies Acc1;1, Acc1;2, and Acc1;3 in wild oat (Christoffers et al., 2002). Note that the sequence region containing the 1781, 2078 or 2088 codon was not included due to infrequent informative SNPs. Boxed regions indicate SNPs discriminating the three haplotypes (GenBank accession AF231335 for Acc1;1-1, AF231334 for Acc1;1-2, AF231336 for Acc1;2 and AF464875 for Acc1;3).
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Title Fig. S4( d)CAPS analysis of clones
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Description Fig. S4 (d)CAPS marker analysis of ACCase mutations at (a) 1781, (b) 2078 or (c) 2088 in representative E. coli clones containing the 1122 kb ACCase cDNA insert. Each lane represents a single clone analysed by each of the three markers. Note that none of these clones contain more than one mutation (R).
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When using this data, please cite the original publication:

Powles S, Yu Q, Ahmad-Hamdani MS, Han H, Christoffers M (2012) Data from: Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): Insights into resistance evolution in a hexaploid species. Heredity 110(3): 220–231. http://dx.doi.org/10.1038/hdy.2012.69

Additionally, please cite the Dryad data package:

Powles S, Yu Q, Ahmad-Hamdani MS, Han H, Christoffers M (2012) Data from: Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): Insights into resistance evolution in a hexaploid species. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.04562
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