Data from: Ungulate saliva inhibits a grass-endophyte mutualism

Tanentzap AJ, Vicari M, Bazely DR

Date Published: July 2, 2014

DOI: http://dx.doi.org/10.5061/dryad.06hn6

 

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Title Ergovaline concentrations in simulated herbivory experiment
Downloaded 15 times
Description Data are from an experiment simulating herbivory and saliva application on 32 Festuca rubra plants infected with Epichloë festucae. We used two simulated herbivory treatments consisting of fortnightly and monthly clipping and a control of no herbivory. Clipped plants received either distilled water or moose saliva. The 32 plants represented 22 isolates (putatively different genets), originating from either the core of F. rubra’s distribution in Europe, mainly Sweden, or its natural southern limit in southern Ontario, Canada. Plants were harvested after growing in a greenhouse for eight weeks and measured for ergovaline concentrations using high-performance liquid chromatography at the Endophyte Service Laboratory, Oregon State University.
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Title Radii of Epichloë festucae colonies treated with saliva or water measured every 12 hours
Downloaded 13 times
Description We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 12 hours over 2 days.
Download ColonyExpt1_TanentzapDryad.csv (10.34 Kb)
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Title Radii of Epichloë festucae colonies treated with saliva or water measured every 8 hours
Downloaded 12 times
Description We tested whether application of reindeer and moose saliva reduced in vitro growth of Epichloë festucae colonies relative to a control treatment of distilled water. We isolated E. festucae from Festuca rubra, grown from natural populations in northern Sweden, by cutting the base of the youngest fully-extended leaf in each plant. Sections were surface sterilized and embedded in potato dextrose medium with 60 mg/L of penicillin G and streptomycin. Plates were stored in the dark at 24°C for 2 weeks. We then assigned 22-25 colonies per treatment, with 5-9 replicates of three plant genotypes, and recorded the initial radius of each plate. Saliva was applied and we measured colony radius relative to the baseline every 8 hours over 2 days.
Download ColonyExpt2_Dryad.csv (14.05 Kb)
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When using this data, please cite the original publication:

Tanentzap AJ, Vicari M, Bazely DR (2014) Ungulate saliva inhibits a grass-endophyte mutualism. Biology Letters 10(7): 20140460. http://dx.doi.org/10.1098/rsbl.2014.0460

Additionally, please cite the Dryad data package:

Tanentzap AJ, Vicari M, Bazely DR (2014) Data from: Ungulate saliva inhibits a grass-endophyte mutualism. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.06hn6
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