Data from: Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment

Aylagas E, Borja A, Irigoien X, Rodriguez-Ezpeleta N

Date Published: June 1, 2016

DOI: http://dx.doi.org/10.5061/dryad.0sc0s

 

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Title Taxonomic assignments using mlCOI barcode
Downloaded 10 times
Description The file contains the taxonomic assignments for reads obtained using mlCOI barcode across all stations for each sample source, DNA type (Extracellular, Bulk or Pooled DNA) and PCR annealing temperature (46 °C, 50 °C or, TD). Ecological Group (EG) for each taxa according AMBI is detailed and number of reads transformed into relative proportions shown. Presence/absence of taxa found using traditional identification at each station is represented by x.
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Title Taxonomic assignments using folCOI barcode
Downloaded 3 times
Description The file contains the taxonomic assignments for reads obtained using folCOI barcode across all stations for each sample source, DNA type (Extracellular, Bulk or Pooled DNA) and PCR annealing temperature (46 °C, 50 °C or, TD). Ecological Group (EG) for each taxa according AMBI is detailed and number of reads transformed into relative proportions shown. Presence/absence of taxa found using traditional identification at each station is represented by x.
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Title Unique Sequences mlCOI barcode
Downloaded 6 times
Description Following Illumina MiSeq sequencing of the mlCOI barcode of 114 samples, 130,670 unique metazoan sequences were identified. The zip file contains a fasta file with all the unique sequences and a table showing the distribution of sequencing reads for all unique sequences across the 114 samples for each DNA type (extracellular (ext), Bulk or Pooled DNA) and PCR annealing temperature (46 °C, 50 °C or, TD).
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Title Unique Sequences folCOI barcode
Downloaded 2 times
Description Following Illumina MiSeq sequencing of the folCOI barcode of 110 samples, 652,356 unique metazoan sequences were identified. The zip file contains a fasta file with all the unique sequences and a table showing the distribution of sequencing reads for all unique sequences across the 110 samples for each DNA type (extracellular (ext), Bulk or Pooled DNA) and PCR annealing temperature (46, 50 or TD).
Download UniqueSequences_folCOI.zip (72.45 Mb)
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When using this data, please cite the original publication:

Aylagas E, Borja A, Irigoien X, Rodriguez-Ezpeleta N (2016) Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment. Frontiers in Marine Science 3: 96. http://dx.doi.org/10.3389/fmars.2016.00096

Additionally, please cite the Dryad data package:

Aylagas E, Borja A, Irigoien X, Rodriguez-Ezpeleta N (2016) Data from: Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.0sc0s
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